TruSeq Stranded mRNA + Ribo-Zero Epidemiology

TruSeq Stranded mRNA + Ribo-Zero Epidemiology

In order to prepare transcriptome libraries from Epidemiology samples, we recommend using a Ribo-Zero rRNA Removal Epidemiology Kits in combination with the TruSeq stranded mRNA kits. This kit will remove cytoplasmic and mitochondrial rRNA from samples composed of human/mouse/rat, Gram-positive and Gram-negative bacterial RNA.

Starting Material: Begin RiboZero with 1-5ug total RNA, then take 10-100ng RiboZero product into TruSeq protocol (see second note in "Purify and Fragment mRNA" section of the User Guide).

LT HT
TruSeq Stranded mRNA Catalog ID(s):
RS-122-2101 (48 samples) (Set A: 12 indices)
RS-122-2102 (48 samples) (Set B: 12 indices)
Ribo-Zero Gold rRNA (Epidemiology) Catalog ID(s):
MRZE706– (6 reactions)
MRZE724– (24 reactions)
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TruSeq Stranded mRNA Catalog ID(s):
RS-122-2103 (96 samples) (96 indices in plate format)
The index plate is single use per well and cannot undergo more than four freeze/thaw cycles. We strongly recommend processing at least 24 samples at a time
Ribo-Zero Gold rRNA (Yeast) Catalog ID(s):
MRZE706 – (6 reactions)
MRZE724 – (24 reactions)
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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Based on 20M reads per sample for RNA profiling and 50M reads per sample for transcriptome analysis. Use twice the number of reads for FFPE or degraded samples.
Project Recommendations
Species Compatibility Human, Mouse, Rat with bacteria
Test other species compatibility with MatchMaker™ Tool
Recommended Illumina Instrument* NextSeq 500 HiSeq 2500 (Dual Flow Cell)
Recommended Samples per run† RNA Profiling: 6–20
Transcriptome Analysis: 2–8		 RNA Profiling: 30–200
Transcriptome Analysis: 12–80
Run Mode Mid Output High Output Rapid Run High Output
Max Recommended Read Length 2x75 bp 2x75 bp 2x75 bp 2x75 bp
Recommended Secondary Analysis
  • TopHat Alignment BaseSpace App
  • Cufflinks Assembly & DE BaseSpace App
  • RNA Express v1.0 BaseSpace App
Recommended Down Stream Analysis
  • Genomatix Pathway
  • iPathway Guide
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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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