TruSeq Stranded mRNA
TruSeq Stranded mRNA
LT HT
TruSeq Stranded mRNA LT isolates poly-A mRNA using oligo dT beads and preserves strandedness of transcripts. Strand information is useful for detection of antisense transcription, transcript annotation, and increased alignment efficiency.
Starting material: Optimized for 100 ng total RNA, up to 1ug total RNA can be used, or entire fraction of mRNA purified from 0.1-1ug total RNA.
TruSeq Stranded mRNA isolates poly-A mRNA using oligo dT beads and preserves strandedness of transcripts. Strand information is useful for detection of antisense transcription, transcript annotation, and increased alignment efficiency.
Starting material: Optimized for 100 ng total RNA, up to 1ug total RNA can be used, or entire fraction of mRNA purified from 0.1-1ug total RNA.
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| Species Compatibility | Any high-quality RNA species with polyA tail | ||||||
|---|---|---|---|---|---|---|---|
| Recommended Illumina Instrument | NextSeq 500 | HiSeq 2500 (Dual Flow Cell) | NovaSeq 6000 (Dual Flow Cell)† | ||||
| Recommended Samples per run (RNA Profiling / Transcriptome Analysis)‡ | 13–40 / 5–16 | 60–400 / 24–160 | 320 / 128 | 384 / 256 | 768 / 768 | ||
| Run Mode | Mid Output | High Output | Rapid Run | High Output | S1§ | S2 | S4 |
| Max Recommended Read Length | 2×75 bp | 2×75 bp | 2×75 bp | 2×75 bp | ≤ 2×100 bp | ||
| Recommended Secondary Analysis |
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| Recommended Down Stream Analysis |
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* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Limited by index combinations (HT). Based on 10M / 25M reads.
‡ Based on 10M reads per sample for RNA profiling and 25M reads per sample for transcriptome analysis.
§ NovaSeq S1 Reagent Kits are not currently available.