The TruSight Tumor sequencing panel takes a deeper view of variation in solid tumors including lung, colon, melanoma, gastric, and ovarian. This kit enables clinical researchers to identify low-frequency variation across 26 genes for a more comprehensive view of somatic variation. Low limit of detection of FFPE samples is obtained by creating two libraries per sample: one for the forward strand and one for the reverse strand. Illumina recommends running 4 samples (8 libraries) per MiSeq v2 run to obtain a minimum coverage of 1000x per amplicon.
TruSight Tumor Gene List
AKT1 ALKPlease rotate your device for details
* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application. † Based on > 1,000x per amplicon. |
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Project Recommendations | |||||||
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Species Compatibility | Human | ||||||
Recommended Illumina Instrument* | MiniSeq | MiSeq | |||||
Recommended Samples per run† | 2 | 6 | 4 (v2 chemistry) | ||||
Run Mode | Mid Output | High Output | - | ||||
Max Recommended Read Length | Up to 2x121 bp | Up to 2x121 bp | Up to 2x121 bp | ||||
Recommended Secondary Analysis |
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Recommended Down Stream Analysis | VariantStudio |