TruSight Tumor 26

TruSight Tumor 26

The TruSight Tumor sequencing panel takes a deeper view of variation in solid tumors including lung, colon, melanoma, gastric, and ovarian. This kit enables clinical researchers to identify low-frequency variation across 26 genes for a more comprehensive view of somatic variation. Low limit of detection of FFPE samples is obtained by creating two libraries per sample: one for the forward strand and one for the reverse strand. Illumina recommends running 4 samples (8 libraries) per MiSeq v2 run to obtain a minimum coverage of 1000x per amplicon.

TruSight Tumor Gene List

AKT1 ALK
FGFR2
APC
FOXL2
BRAF
GNAQ
CDH1 CTNNB1 EGFR ERBB2 FBXW7 GNAS KIT
PTEN
KRAS
SMAD4
MAP2K1
SRC
MET MSH6 NRAS PDGFRA PIK3CA STK11 TP53

Please rotate your device for details

* Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
Based on > 1,000x per amplicon.
Project Recommendations
Species Compatibility Human
Recommended Illumina Instrument* MiniSeq MiSeq
Recommended Samples per run 2 6 4 (v2 chemistry)
Run Mode Mid Output High Output -
Max Recommended Read Length Up to 2x121 bp Up to 2x121 bp Up to 2x121 bp
Recommended Secondary Analysis
  • Local Run Manager Amplicon DS
  • BaseSpace Amplicon DS
  • MiSeq Reporter AmpliconDS Workflow
Recommended Down Stream Analysis VariantStudio