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| AmpliSeq for Illumina BRCA Panel | TruSight Cancer | |
|---|---|---|
| Key Use | Germline and somatic analysis of BRCA1 and BRCA2. | Germline mutation detection for common and rare cancers. |
| Design | All exonic regions of the BRCA1 and BRCA2 tumor supressor genes and flanking intronic sequences. | Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. |
| Library Prep Time | 6 hours | 12 hours |
| Genomic Content | 22 kb | 225 kb (94 genes, 284 SNPs) |
| Input Amount | 10ng recommended | 50ng gDNA |
| FFPE Compatible | Yes | No |
| Multiplexing | Up to 96-plex | Up to 96-plex |
| Method | Amplicon | Enrichment |