Comparison Table
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| TruSeq DNA PCR-Free | TruSeq DNA Nano | Nextera DNA | Nextera DNA Flex | |
|---|---|---|---|---|
| Intended Use | Leverages a workflow similar to TruSeq DNA, but without PCR. Is a simple, all-inclusive prep for whole genome sequencing with the industry best genome coverage and ability to sequence through challenging regions of the genome. | A low-input method that delivers high genome coverage quality and reduced bias. | A fast and easy workflow delivering sequencing-ready results in 90 minutes. | Illumina’s fastest and easiest workflow from sample to normalized library |
| Input Quantity | 1ug/2ug | LT/HT: 100ng/200ng | 50ng | 1–500ng |
| Assay Time* | ~5 hours | LT/HT: ~5.5 hours | ~1.5 hours | 3–3.5 hours (from blood/saliva DNA extraction to normalized library) |
| Multiplexing | LT Kit: 24 HT Kit: 96 |
LT Kit: 24 HT Kit: 96 |
96plex | 96 dual indexes |
| Fragmentation | Mechanical | Enzymatic | Enzymatic | |
| Target Insert Size | 350bp or 550bp | 300bp–1.5kb | ~500bp | |
| Bisulfite Compatibility | LT Kit: Yes, adapters are methylated HT Kit: No |
No | No | |
* Assay time for TruSeq PCR Free, TruSeq DNA Nano, and Nextera DNA do not include DNA extraction time or library normalization time.