Method Category: Transcriptome > RNA Low-Level Detection
Description: Unique molecular identifiers (UMI) are molecular tags that are used to detect and quantify unique mRNA transcripts. In this method, mRNA libraries are generated by fragmentation and reverse-transcribed to cDNA. Oligo(dT) primers with specific sequencing linkers are added to the cDNA. Another sequencing linker with a 10 bp random label and an index sequence is added to the 5' end of the template, which is amplified and sequenced. Sequencing allows for high-resolution reads, enabling accurate detection of true variants.