With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.
Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.
The IDT® for Illumina®-Nextera™ DNA UD Indexes Set A is the latest set of indexes, offering up to 96 unique dual indexes to enable accurate assignment of reads and efficient use of the flow cell.
The IDT for Illumina - Nextera DNA UD Indexes use 10 base pair (bp) index codes. This index code change requires adjustments to the sequencing run setup. See the Nextera DNA UD indexes support page for additional information.
Find automation vendors with robotic systems compatible with this kit
For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.
Read Tech NoteInstrument | Recommended Number of Samples | Read Length |
---|---|---|
MiSeq System | Up to 384 samples per run | Up to 2 x 300 bp |
NextSeq 550 System | Up to 384 samples per run | Up to 2 x 150 bp |
Nextera XT DNA Library Preparation Kit | Nextera DNA Flex Library Prep Kit | |
---|---|---|
Assay Time | ~5.5 hours from DNA extraction to normalized library. (Library prep time: ~90 minutes). | ~3-4 hours (from DNA extraction to normalized library) |
Description | Fast library prep optimized for research on small genomes, PCR amplicons, and plasmids. | A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more. |
Hands-On Time | 15 minutes | 1-1.5 hours |
Input Quantity | 1 ng DNA | Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. |
Mechanism of Action | Enzymatic fragmentation | Bead-linked transposome |
Method | 16s rRNA Sequencing, Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing | Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing |
Multiplexing | Up to 384 uniquely indexed samples may be pooled and sequenced together. | Up to 96 available indexes |
Specialized Sample Types | Low-Input Samples, Not FFPE-Compatible, Single Cells | Blood, Not FFPE-Compatible, Saliva |
Species Details | Compatible with any species | Compatible with any species |
Target Insert Size | 300 bp–1.5 kb | ~500 bp |
Single-cell mRNA-Seq with the MiSeq and HiSeq Systems is enabling Allen Institute researchers to classify V1 neural cells.
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Technical Note | PDF < 1 MB
Nextera Low Plex Pooling Guidelines
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Nextera XT Library Prep: Tips and Troubleshooting
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Nextera XT DNA Library Preparation Kit
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