Nextera XT DNA Library Preparation Kit

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids

• Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
• Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
• Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
• Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

The IDT® for Illumina®-Nextera™ DNA UD Indexes Set A is the latest set of indexes, offering up to 96 unique dual indexes to enable accurate assignment of reads and efficient use of the flow cell.

The IDT for Illumina - Nextera DNA UD Indexes use 10 base pair (bp) index codes. This index code change requires adjustments to the sequencing run setup. See the Nextera DNA UD indexes support page for additional information.

Find automation vendors with robotic systems compatible with this kit

This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.