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NextSeq Series

Choose the Right Sequencer
Choose the Right Sequencer

For tips about how to choose the best sequencer and transform your lab, download the Buyer's Guide to Next-Generation Sequencing Systems.

Flexible High-Throughput Sequencing Power on Your Benchtop
Flexible High-Throughput Sequencing Power on Your Benchtop

The NextSeq 500 benchtop sequencer delivers on-demand exome, transcriptome and whole-genome sequencing.
Discover the NextSeq 550 System
Discover the NextSeq 550 System

Watch this webinar to learn about the integration of NGS and array applications on the NextSeq 550 System.

The NextSeq Online Community

Join other NextSeq owners in the NextSeq Online Community. Collaborate with Illumina moderators and NextSeq owners. Discuss best practices, troubleshoot, and learn about how others are using NextSeq preparation kits, push-button sequencing, and automated data analysis to fuel their research.
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Illumina NextSeq Community

High-throughput benchtop sequencing

The NextSeq 550 System delivers the power of high-throughput sequencing with the speed, simplicity, and affordability of a benchtop next-generation sequencing (NGS) system. The NextSeq 550 System fits into research laboratories, without need for specialized equipment. Perform many combinations of high- and mid-throughput sequencing applications to advance your studies.

Download Data Sheet

Next: Flexible, scalable NGS system for virtually any application

Flexible, scalable NGS system for virtually any application

The NextSeq 550 System is efficient and flexible to handle a range of different types of projects. Perform whole-genome, transcriptome, and targeted resequencing applications with a rapid, one-day turnaround. Easily switch between two flow cell configurations to tune your output based on your current research needs for applications or sample batch sizes.

Explore Applications

Next: Transition from array scanning to sequencing

Transition from array scanning to sequencing

Perform next-generation sequencing (NGS) and cytogenomic microarray scanning all on one research instrument. The NextSeq 550 System combines high-quality array scanning with widely adopted Illumina sequencing technology. By leveraging array scanning on the NextSeq 550 System, researchers have instant access to a powerful, complementary technology to confirm copy number variants detected through sequencing.

Download Data Sheet

Next: Simplified informatics

Simplified informatics

To streamline run management, downstream analysis, and data sharing, the NextSeq 550 System is integrated with BaseSpace Sequence Hub. This Illumina informatics solution, available in both cloud and on-site packages, enables researchers to go from sample to results without bioinformatics expertise.

Explore BaseSpace Sequence Hub

Start Over: High-throughput benchtop sequencing
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Workflow

 
Prep

Support for a broad range of applications

 
Sequence

Automated sequencing to generate data in less than a day

 
Analyze

Data transferred automatically to BaseSpace Sequence Hub for simplified analysis

 
Share

Secure, expandable storage, and ability to share data instantly

See NextSeq Application-Specific Workflows

System Specifications

16.25–120 Gb

output range

130–400 million

reads per run

2 x 150 bp

max read length
View All NextSeq Specifications

Customer Stories

NextSeq Series for RNA Sequencing in Virology
NextSeq Series for RNA Sequencing in Virology

Learn how this lab is leveraging the power of Illumina next-generation sequencing for their cutting-edge work in virology.

View Video
Seeking the Source of Bacterial Drug Resistance

A targeted deep sequencing assay identifies multidrug-resistant tuberculosis strains responsible for silent outbreaks.

Read Article
Identifying lncRNA Biomarkers in Highly Fragmented RNA Samples

Optimizing NextSeq 500 System and TruSeq RNA Exome Kit RNA sequencing workflows for lncRNA biomarker identification.

Read Article
Read More Stories
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Featured Products

NextSeq 500/550 v2.5 Kits
NextSeq 500/550 v2.5 Kits

These sequencing reagent kits offer increased stability and robustness over the v2 kit version.

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Nextera XT DNA Library Preparation Kit
Nextera XT DNA Library Preparation Kit

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.

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TruSeq Stranded mRNA
TruSeq Stranded mRNA

Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.

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TruSight Oncology 500
TruSight Oncology 500

Assay targeting multiple variant types, including tumor mutational burden (TMB) and microsatellite instability (MSI), even from low-quality samples.

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Related Solutions

The NextSeq 500 Sequencing System has been discontinued. The NextSeq 550 System is an alternative solution that provides the increased flexibility of microarray scanning in addition to sequencing.

Innovative technologies

At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics.

For Research Use Only. Not for use in diagnostic procedures (except as specifically noted).

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