TruSight RNA Pan-Cancer Panel
TruSight RNA Pan-Cancer Panel
The TruSight RNA Pan-Cancer Panel enables comprehensive detection of gene fusions, variants and gene expression changes in cancer samples, providing researchers with a focused view of the functionally relevant changes occurring. The panel is compatible with FFPE tissue, accommodating as little as 10 ng of fresh-frozen total RNA input or 20ng of FFPE RNA input. The TruSight RNA Pan-Cancer Panel provides a sensitive, reproducible, and economical solution for studies of expression dynamics and functional mechanisms in cancer.
Starting material:
The protocol is optimized for 10–100 ng of human total RNA.
- Lower amounts might result in low yield and reduced sensitivity.
- The protocol has been tested using 10 ng of high-quality universal human reference total RNA as input.
- Use of RNA from other tissues or lower quality RNA might require further optimization to determine the input amount.
| Quality | DV200 | Input Requirements Per Reaction |
|---|---|---|
| High | > 70% | 20 ng |
| Medium | 50–70% | 20–50 ng |
| Low | 30–50% | 50–100 ng |
| Too Degraded | < 30% | Not recommended |
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| Species Compatibility | Human | |||
|---|---|---|---|---|
| Recommended Illumina Instrument* | MiniSeq | MiSeq | ||
| Recommended Samples per Run† | 2 to 3 | 8 | 8 (v3 chemistry) | |
| Max Recommended Read Length | 2×75 bp | 2×75 bp | 2×75 bp | |
| Recommended Secondary Analysis | RNA-seq (FASTQ) | RNA-seq (FASTQ) | ||
| Recommended Down Stream Analysis | BaseSpace RNA-Seq Alignment App | BaseSpace RNA-Seq Alignment App | ||
*Library prep is compatible with all Illumina instruments. Recommended instruments chosen based on typical output and analysis need for selected application.
† Based on 3M reads per sample.
Product and Ordering Information
- TruSight RNA Pan-Cancer Panel Set A (48 samples): RS-303-1002
- TruSight RNA Pan-Cancer Panel Set B (48 samples): RS-303-1003