Comparison Table
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| Nextera Mate Pair | TruSeq Synthetic Long-Read | |
|---|---|---|
| Intended Use | The only gel-free method for preparing up to 12 kb mate pair libraries with the industry’s lowest DNA input requirement. It is an ideal approach for aligning long paired ends for de novo assembly and detection of structural variation. | Synthetic long fragment library preparation for assembly into long-reads or whole human genome phasing. Provides greater accuracy for de novo genome assembly, genome finishing studies, and the ability to distinguish between variants on homologous chromosomes. |
| Input Amount | Gel-Free: 1 ug Gel-Plus: 4 ug |
500 ng |
| Multiplexing | 12plex | Not available |
| Methodology | Methods that target the junction site of large circularized fragments. | Methods that randomly shear gDNA to produce long fragments. These large fragments are then tagged with Nextera enzymes for assembly downstream. |
| Instrument Compatibility | HiSeq, NextSeq, MiSeq, GAII | HiSeq |
| Analysis | 3rd party software capable of analyzing mate-pair reads | TruSeq Long-Read Assembly App or TruSeq Phasing Analysis App |