Sequencing, reimagined
The most accurate genome with expanded insights and unmatched simplicity
Illumina TruPath Genome transforms whole-genome sequencing (WGS) by combining the accuracy and accessibility of short-read sequencing with long-range insights traditionally limited to long-read platforms. TruPath Genome delivers an ultra-simple workflow, unlocking challenging genomic regions, structural variants, and phased variant calls across the human genome.
Video
Steve Barnard, PhD, CTO and Head of Research & Product Development at Illumina, introduces TruPath Genome at AGBT 2026 and discusses how this transformative solution delivers an ultra-simple workflow—with ~10 minutes hands-on time—unlocking challenging genomic regions, structural variants, and phased variant calls across the human genome.
Up to 16 genomes per run
~10 min hands-on time
>4,000 genomes per year (>30× coverage)
Compared to current long‑read approaches, TruPath Genome delivers up to four times more genomes per run and cuts end‑to‑end turnaround time nearly in half.
By nearly eliminating hands-on sample prep, accelerating sequencing, and dramatically reducing turnaround time without compromising data quality, TruPath Genome enables generation of up to 4,000 genomes per year (with greater than 30× coverage) on a single NovaSeq X Plus System.
Powered by proximity mapped read technology, TruPath Genome enables human germline whole-genome sequencing with improved coverage, higher accuracy variant detection, and unprecedented simplicity. On-flow-cell library prep eliminates standard library prep prior to sequencing.
Highly accurate, comprehensive germline human whole-genome sequencing with only ~10 min of hands on time, without traditional library prep.
The NovaSeq X Series delivers extraordinary throughput and accuracy to perform data-intensive applications at production scale.
DRAGEN Germline is an accurate and efficient (FASTQ to VCF) secondary analysis solution for whole genome, whole exome, and targeted panel NGS data.
Explainable, evidence-linked prioritization leveraging phased variants for visualization, filtering and sorting.
Illumina TruPath Genome transforms whole-genome sequencing (WGS) by combining the accuracy and accessibility of short-read sequencing with long-range insights traditionally limited to long-read platforms. TruPath Genome delivers an ultra-simple workflow, unlocking challenging genomic regions, structural variants, and phased variant calls across the human genome.
Mapping the future of WGS
Joseph Devaney, PhD, Director of Laboratory Innovation Team at GeneDx, compares data and shares his perspectives on the potential impact of proximity mapped read (formerly constellation) technology.
Perspective: Experience with TruPath Genome
Marcel Nelen, PhD, Head of Genome Diagnostics at UMC Utrecht, discusses the types of studies that may benefit from mapped read technology.
Deeper insight into complex genomic regions
Steve Barnard PhD, CTO and Head of Research & Product Development at Illumina, outlines the research challenges Illumina TruPath Genome is designed to address.
Learn how TruPath Genome can help you unlock long-distance genomic insights.
