The cBot 2 System is a revolutionary automated system that creates clonal clusters from single molecule DNA templates, preparing them for sequencing by synthesis on HiSeq and HiSeq X Series sequencers. The system allows for streamlined cluster generation and positive sample tracking, enabling clear and controlled sample identification through the sequencing workflow.
Cluster Generation Process
The cBot 2 System isothermally amplifies cDNA fragments that have been captured by complementary adapter oligonucleotides covalently bound to the surface of Illumina flow cells. Flow cells facilitate access of bound DNA to enzymes while ensuring high stability of surface-bound template and low non-specific binding of fluorescently-labeled nucleotides.
Attached DNA fragments are extended and bridge-amplified to create hundreds of millions of clusters, each of which contains ~1000 identical copies of a single template molecule.
Sample Tracking and LIMS Capabilities
The cBot 2 System incorporates 2 internal barcode readers to track samples for end-to-end traceability. Unique strip tube barcodes identify the position of each sample as it moves through the sequencing workflow, maintaining sample traceability throughout the cluster generation process.
This information can be integrated directly with a laboratory information management system (LIMS). If sample traceability is not required, the cBot 2 System can be used in standard mode.