Illumina Free Adapter Blocking Reagent

An enzymatic solution to block free adapters from library pools, reducing index hopping rates and the potential for misalignment.Read More...
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Illumina Free Adapter Blocking Reagent (12 reactions)

20024144

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Illumina Free Adapter Blocking Reagent (48 reactions)

20024145

Price
 
 

Product Highlights

Index hopping can occur when index sequences are incorrectly assigned to the libraries within a multiplexed pool, leading to misalignment of sequencing data. If unligated adapters are not removed after adapter ligation, they can contaminate libraries and contribute to higher index hopping rates.

The Illumina Free Adapter Blocking Reagent minimizes index hopping by blocking unligated adapters, preventing them from hybridizing and producing index-hopped strands. This enzymatic treatment can be performed on a single library or a pool of libraries after library preparation, before the denaturation step.

The Illumina Free Adapter Blocking Reagent features:

  • A universal solution for treating multiple library types
  • A simple, easy-to-use protocol
  • Flexibility to pool up to 96 libraries per treatment

Specifications

Supporting Data and Figures

Reduced Index Hopping with Illumina Free Adapter Blocking Reagent
Libraries were prepared and pooled following the TruSeq DNA PCR-Free protocol. Adapter spike-in induced a high index hopping rate of 6.94%. Treatment with the Illumina Free Adapter Blocking Reagent after adapter spike-in reduced index hopping to a baseline rate below 1%, comparable to the treated control libraries.
 

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