We recommend ordering the new Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are obsolete and the last order date is January 30, 2025, as announced in PON2023-1440.
TruSight One and TruSight One Expanded enrichment oligos are now available separately for use with Illumina DNA Prep with Enrichment. Library prep for TruSight One (FC-141-1006 and FC-141-1007) and TruSight One Expanded (FC-141-2007) has been discontinued.
TruSight One Sequencing Panels enable labs to expand and streamline their sequencing portfolio, while managing costs.
These panels provide clinical research labs with an affordable solution for managing a diverse assay portfolio. The TruSight One Sequencing Panel provides comprehensive coverage of > 4800 disease-associated genes, while the TruSight One Expanded Sequencing Panel targets ~1900 additional genes with recent disease associations in the scientific literature.
Investigators can choose to analyze all of the genes on the panel or focus on a specific subset. With a single assay, labs can expand existing menus, streamline workflows, or create an entire portfolio of sequencing options.
Download this reference guide to understand which kit quantities you should purchase to support your research.
Download Reference Guide
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| MiSeq System | TruSight One: 3 samples per run with v3 reagents (based on 100x mean coverage of targeted content) | Up to 2 × 150 bp |
| NextSeq 550 System | TruSight One (samples per run): mid output: 12, high output: 36 (based on 100x mean coverage of targeted content) TruSight One Expanded: 24 samples per run (high output; based on 100x mean coverage of targeted content) |
Up to 2 × 150 bp Up to 2 × 150 bp |
| NovaSeq 6000 System | TruSight One Expanded: 96 samples per run (S1 flow cell; based on 100x mean coverage of targeted content) | Up to 2 × 150 bp |
| NovaSeq 6000 System | TruSight One Expanded: 96 samples per run (S1 flow cell; based on 100x mean coverage of targeted content) | Up to 2 × 150 bp |
| TruSight One Sequencing Panels | TruSight Cardio Sequencing Panel | |
|---|---|---|
| Content Specifications | TruSight One: ~12 Mb genomic content (~4800 genes). TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes). |
575 Kb genomic content (174 genes) |
| Description | Fixed research panels targeting exonic regions that harbor disease-causing variants. | Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions. |
| Input Quantity | 50 ng DNA | 50 ng DNA |
| Method | Target Enrichment, Target Enrichment, Targeted DNA Sequencing | Target Enrichment, Target Enrichment, Targeted DNA Sequencing |
| Multiplexing | Up to 96-plex | Up to 96-plex |
| Specialized Sample Types | Not FFPE-Compatible | Not FFPE-Compatible |
| Species Category | Human | Human |
Due to the larger size of the TruSight One Expanded Panel, Illumina recommends using a NextSeq, HiSeq, or NovaSeq system to achieve optimal sequencing coverage.
Dr. Kenjiro Kosaki furthers his clinical research and expands the genetic analysis services he provides with the TruSight One Sequencing Panel.
Read MoreSequencing Panel for 4813 Genes with Known Associated Clinical Phenotypes
Technical Note | PDF < 1 MB
marker_list_-csv,_zip- | ZIP < 1 MB
TruSight One Expanded Panel Gene List
marker_list_-csv,_zip- | EXCEL < 1 MB
TruSight Portfolio: New Possibilities for Next-Generation Sequencing
Data Sheet | PDF < 1 MB
TruSight One Sequencing Panel Kits Support Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All Illumina DNA RNA UD Indexes Support All TruSight One Sequencing Panel Kits Support
Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier MiSeq reagent kit versions.
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.
The NovaSeq Xp workflow enables labs to load libraries directly into each lane of a NovaSeq 6000 System flow cell, without additional instrumentation.
Assay targeting multiple variant types, including microsatellite instability (MSI) and tumor mutational burden (TMB).
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
