Comprehensive sequencing research panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.
Assay time
Hands-on time
Input quantity
TruSight One and TruSight One Expanded enrichment oligos are now available separately for use with Illumina DNA Prep with Enrichment. Library prep kits for TruSight One (Catalog no. FC-141-1006 and FC-141-1007) and TruSight One Expanded (Catalog no. FC-141-2007) have been discontinued.
TruSight One Sequencing Panels deliver broad content and high coverage for disease-associated genes based on multiple genomic databases and guidance from industry experts.
Two panel options are available, providing clinical research labs with an affordable solution for managing a diverse assay portfolio.
This panel provides comprehensive coverage of 12 Mb of genomic content, including > 4800 disease-associated genes linked to specific clinical phenotypes, enabling researchers to focus on genes with known disease associations.
This panel further optimizes the original panel to improve coverage. The Expanded Panel targets ~1900 additional genes in addition to the original > 4800, for a total of 16.5 Mb of genomic content.
| Assay time | 6.5 hr |
|---|---|
| Content specifications |
TruSight One: ~12 Mb genomic content (~4800 genes). TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes). |
| Hands-on time | ~2 hr |
| Input quantity | 50 ng DNA |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System, NovaSeq 6000 System |
| Method | Targeted DNA sequencing, Target enrichment |
| Multiplexing | Up to 96-plex |
| Nucleic acid type | DNA |
| Specialized sample types | Not FFPE-compatible |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Germline variants |
Requires either:
A purchase of the TruSight One Sequencing Combo bundled solution (supports 15 samples and contains 1 TruSight One – Enrichment Oligos kit, 1 Illumina DNA Prep with Enrichment, (S) Tagmentation 16 samples, and 5 MiSeq Reagent Kits v3, 600 cycles), or
Separate purchases of enrichment oligos for each panel, Illumina DNA Prep with or without Enrichment for 16 or 96 samples, and sequencing reagents for your system
The IDT for Illumina DNA/RNA UD Indexes (A–D) are a required separate purchase whether you opt for the Combo bundle solution or purchase enrichment and library prep separately.
TruSight One Sequencing Panels provide extensive content and high coverage of disease-associated target regions, enabling labs to expand existing menus, streamline workflows, and replace iterative testing with a single assay.
TruSight One Sequencing Panels
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| MiSeq System | TruSight One: 3 samples per run with v3 reagents |
Up to 2 × 150 bp |
| NextSeq 550 System | TruSight One (samples per run): mid output: 12, high output: 36 TruSight One Expanded: 24 samples per run (high output) |
Up to 2 × 150 bp Up to 2 × 150 bp |
| NextSeq 2000 System | TruSight One: 96 samples per run (P3 flow cell) TruSight One Expanded: 66 samples per run (P3 flow cell) |
Up to 2 × 150 bp Up to 2 × 150 bp |
| NovaSeq 6000 System | TruSight One Expanded: 96 samples per run (S1 flow cell) |
Up to 2 × 150 bp |
The recommended number of samples is based on 100× mean coverage of targeted content.
Complex and genetic disease research
Comprehensive array and next-generation sequencing solutions to accelerate research of various genetic complex diseases.
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Target enrichment captures genomic regions of interest through hybridization. Learn more about target enrichment and how it can be used to assess multiple samples at once.
| TruSight One Sequencing Panels | Illumina DNA Prep with Exome 2.5 Enrichment | ||||
|---|---|---|---|---|---|
| Assay time | 6.5 hr | 6.5 hr | |||
| Content specifications |
TruSight One: ~12 Mb genomic content (~4800 genes). TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes). |
Twist Bioscience for Illumina Exome 2.5 Exome 2.5 Panel 37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM Twist Bioscience for Illumina Mitochondrial Panel covers the circular mitochondrial genome ChrM (16,659 bp, 37 genes) |
|||
| Hands-on time | ~2 hr | ~2 hr | |||
| Input quantity | 50 ng DNA | 50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide) | |||
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System, NovaSeq 6000 System | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000 System, NovaSeq X Plus System | |||
| Method | Targeted DNA sequencing, Target enrichment | Targeted DNA sequencing, Exome sequencing, Target enrichment | |||
| Multiplexing | Up to 96-plex | Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible. | |||
| Nucleic acid type | DNA | DNA | |||
| Specialized sample types | Not FFPE-compatible | Blood, Saliva | |||
| Species category | Human | Human | |||
| Technology | Sequencing | Sequencing | |||
| Variant class | Single nucleotide polymorphisms (SNPs), Germline variants | Single nucleotide polymorphisms (SNPs), Loss of heterozygosity (LOH), Somatic variants, Germline variants, Insertions-deletions (indels), Copy number variants (CNVs) |
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
TruSight One Sequencing Panels global gene content contributors
The TruSight One panels focus on exonic regions of the genome with known disease-associated variants. Combining data from multiple public sources makes sure that the panels cover all genes currently reviewed in the clinical research setting.
TruSight One and Illumina tagmentation chemistry
The TruSight One enrichment oligos work with Illumina on-bead tagmentation chemistry to provide a fast, simple method for enrichment of targeted genes. The workflow combines library preparation and target enrichment steps and can be completed in 1.5 hours.
Hear about the collaborative approach to developing TruSight content sets.
TruSight One Sequencing Combo (15 Samples)
20042621
Sufficient for 15 samples and includes TruSight One – Enrichment Oligos (Cat. no 20029227), Illumina DNA Prep with Enrichment, (S) Tagmentation (16 samples) (Cat. no. 20025523), and five MiSeq Reagent Kits v3 (600-cycle) (Cat. no. MS-102-3003). Purchase IDT for Illumina DNA/RNA UD Indexes separately.
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TruSight One – Enrichment Oligos only (6 Enrichment Reactions)
20029227
Sufficient for six enrichment reactions when paired with Illumina DNA Prep with Enrichment, (S) Tagmentation. Purchase library prep and enrichment reagents and index adapters separately.
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TruSight One Expanded – Enrichment Oligos only (6 Enrichment Reactions)
20029226
Sufficient for six enrichment reactions when paired with Illumina DNA Prep with Enrichment, (S) Tagmentation. Purchase library prep and enrichment reagents and index adapters separately.
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Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)
20025523
Includes reagents for preparing and enriching 16 libraries (16, 1-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.
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Illumina® DNA Prep, (S) Tagmentation (16 Samples)
20025519
Includes reagents for preparing 16 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (16 samples)).
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Illumina® DNA Prep, (S) Tagmentation (96 Samples)
20025520
Includes reagents for preparing 96 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (96 samples)).
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Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)
20025524
Includes reagents for preparing and enriching 96 libraries (eight, 12-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.
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Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)
20091654
Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)
20091656
Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)
20091658
Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)
20091660
Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)
20027213
Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)
20027214
Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)
20042666
Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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IDT® for Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)
20042667
Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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