Products

TruSight One Sequencing Panels

Comprehensive sequencing research panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.Read More...
Select Product(s)
Bundled Solutions

TruSight One Sequencing Combo (15 Samples)

20042621

Price
 
 
Enrichment Panels

TruSight One – Enrichment Oligos only (6 Enrichment Reactions)

20029227

Price
 
 

TruSight One Expanded – Enrichment Oligos only (6 Enrichment Reactions)

20029226

Price
 
 
Library Prep

Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)

20025523

Price
 
 

Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)

20025524

Price
 
 

Illumina® DNA Prep, (S) Tagmentation (16 Samples)

20025519

Price
 
 

Illumina® DNA Prep, (S) Tagmentation (96 Samples)

20025520

Price
 
 
Index Adapters

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

Price
 
 

IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

Price
 
 

IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

Price
 
 

IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

Price
 
 
Add To Cart
Total:

TruSight One Sequencing Panels

Innovation Through Collaboration

Transforming the Clinical Laboratory

Product Highlights

TruSight One Sequencing Panels enable labs to expand and streamline their sequencing portfolio, while managing costs.

  • Two panels offer options to target up to 6700 genes associated with human disease, with high coverage
  • Single assay includes multiple genes of interest, replacing iterative testing
  • Easy, flexible gene filtering and annotation options simplify biological interpretation and reporting

These panels provide clinical research labs with an affordable solution for managing a diverse assay portfolio. The TruSight One Sequencing Panel provides comprehensive coverage of > 4800 disease-associated genes, while the TruSight One Expanded Sequencing Panel targets ~1900 additional genes with recent disease associations in the scientific literature.

Investigators can choose to analyze all of the genes on the panel or focus on a specific subset. With a single assay, labs can expand existing menus, streamline workflows, or create an entire portfolio of sequencing options.

Frequently Purchased Together

Plan on Using the TruSight One Enrichment Panel with NextSeq Reagents and Nextera Flex Enrichment?

Download this reference guide to understand which kit quantities you should purchase to support your research.

Download Reference Guide
Reference guide: TruSight One Enrichment Panel with NextSeq Reagents and Nextera Flex Enrichment

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiSeq System TruSight One: 3 samples per run with v3 reagents (based on 100x mean coverage of targeted content) Up to 2 × 150 bp
NextSeq 550 System TruSight One (samples per run): mid output: 12, high output: 36 (based on 100x mean coverage of targeted content)
TruSight One Expanded: 24 samples per run (high output; based on 100x mean coverage of targeted content)		 Up to 2 × 150 bp
Up to 2 × 150 bp
HiSeq 2500 System TruSight One Expanded (samples per run): rapid run: 18, high output: 95 (based on 100x mean coverage of targeted content) Up to 2 × 150 bp
NovaSeq 6000 System TruSight One Expanded: 96 samples per run (S1 flow cell; based on 100x mean coverage of targeted content) Up to 2 × 150 bp

Product Comparison

TruSight One Sequencing Panels TruSight Inherited Disease TruSight Cardio Sequencing Kit
Content Specifications TruSight One: ~12 Mb genomic content (~4800 genes).
TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).		 2.25 Mb genomic content (552 genes) 575 Kb genomic content (174 genes)
Description Fixed research panels targeting exonic regions that harbor disease-causing variants. Fixed research panel focused on severe pediatric onset disorders. Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions.
Input Quantity 50 ng DNA 50 ng DNA 50 ng DNA
Method Target Enrichment , Target Enrichment, Targeted DNA Sequencing Target Enrichment , Target Enrichment, Targeted DNA Sequencing Target Enrichment , Target Enrichment, Targeted DNA Sequencing
Multiplexing Up to 96-plex Up to 96-plex Up to 96-plex
Specialized Sample Types Not FFPE-Compatible Not FFPE-Compatible Not FFPE-Compatible
Species Category Human Human Human

Due to the larger size of the TruSight One Expanded Panel, Illumina recommends using a NextSeq, HiSeq, or NovaSeq system to achieve optimal sequencing coverage.

Method-Specific Workflow Example

 
Prepare Library for:
Targeted Gene Sequencing
 
Sequence Efficiently with:
HiSeq 4000 System
MiniSeq System
MiSeq System
NextSeq 550 System
NovaSeq 6000 System
 
Analyze Your Data with:
BaseSpace BWA Enrichment App
BaseSpace Enrichment App
VariantStudio Software
BaseSpace Variant Interpreter
 

Customer Stories

Gaining Insight into Genetic Disease

Dr. Kenjiro Kosaki furthers his clinical research and expands the genetic analysis services he provides with the TruSight One Sequencing Panel.

Read More

Product Literature

Sequencing Panel for 4813 Genes with Known Associated Clinical Phenotypes

Technical Note | PDF< 1 MB

TruSight One Panel Gene List

marker_list_-csv,_zip- | ZIP< 1 MB

TruSight One Expanded Panel Gene List

marker_list_-csv,_zip- | EXCEL< 1 MB

Data Sheet | PDF | 5 versions

Manuals and Support Information

TruSight One Sequencing Panel Kits Support Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

All TruSight One Sequencing Panel Kits Support 

Related Products

Related Solutions

Back to Top  

Innovative technologies

At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics.

For Research Use Only. Not for use in diagnostic procedures (except as specifically noted).

© 2021 Illumina, Inc. All rights reserved.

All trademarks are the property of Illumina, Inc. or their respective owners.
For specific trademark information, see emea.illumina.com/company/legal.html.