TruSight Oncology 500 v2

Enable CGP with a large pan-cancer panel covering all main variant classes plus key biomarkers (TMB, MSI, and HRD) from FFPE tissue.

TruSight Oncology 500 v2
Data sheet PDF 3 MB

3–4 days

Assay time

~7 hrs

Hands-on time

30 ng DNA (as low as 10 ng), 40 ng RNA (as low as 20 ng)

Input quantity

See full details in the specifications table

TruSight Oncology 500 v2 automation kits are coming soon. Contact an Illumina representative for more information.

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Overview

TruSight Oncology 500 v2 is a pan-cancer next-generation sequencing (NGS) assay that enables in-house comprehensive genomic profiling (CGP) from formalin-fixed, paraffin-embedded (FFPE) tissue for oncology research.

Targets 523 genes to assess DNA and RNA variant types, including single nucleotide variants, copy number variants, indels, fusions, and large BRCA1/2 rearrangements
Includes analysis of microsatellite instability (MSI), tumor mutational burden (TMB), and homologous recombination deficiency (HRD) biomarkers
Achieves sensitive results from as little as 10 ng DNA or 20 ng RNA using advanced chemistry (30 ng DNA and 40 ng RNA recommended)
Enables rapid turnaround within 3–4 days from sample to results, with a streamlined workflow and automation kits and protocols
Performs fast, scalable variant calling with DRAGEN secondary analysis, available on-premises or via the cloud through Illumina Connected Analytics; enables reporting with Illumina Connected Insights* or Velsera Clinical Genomics Workspace (CGW)
Complements TruSight Oncology 500 ctDNA v2 liquid biopsy assay, with similar content and compatible platforms

Fully integrated HRD testing

HRD is a genomic signature linked to a cell’s inability to repair double-stranded DNA breaks, leading to genomic instability and tumorigenesis. HRD analysis is now integrated into the standard workflow, providing BRCA1/2 variant detection and genomic instability score (GIS) with no added time or complexity.

Automate for efficiency

Illumina has partnered with leading liquid-handling manufacturers to produce fully automated workflows that can reduce hand-on time by ~50%. This improves efficiency while achieving the same high-quality results produced by manual protocols.

^*Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.

Enable CGP with a comprehensive portfolio

Identify disease-relevant biomarkers for oncology research with the TruSight Oncology 500 product line.

TruSight Oncology 500 ctDNA

Analyze circulating tumor DNA (ctDNA) in blood plasma via liquid biopsy with similar DNA panel content as TruSight Oncology 500.

Learn more about the TruSight Oncology 500 product line

Specifications

Assay time	3–4 days from sample input to final results
Automation capability	Liquid handling robot(s)
Automation details	Explore available automation methods
Cancer type	Pan-cancer, Solid tumor
Content specifications	Targeted sequencing of DNA from 523 genes and RNA from 55 genes for a total of 1.94 Mb panel size. MSI and TMB measurement included. The included HRD panel ^† includes coverage of ~25K SNPs to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST) powered by Myriad Genetics. ^†TruSight Oncology 500 v2 is not available for sale in Japan
Description	Enables comprehensive genomic profiling of solid tumors using DNA and RNA from FFPE tissue. Provides variant detection and biomarker assessment from a single sample for broad tumor characterization.
Hands-on time	~7 hrs for manual workflow
Input quantity	30 ng DNA (as low as 10 ng), 40 ng RNA (as low as 20 ng)
Instruments	NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System, NovaSeq X Plus System
Method	Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment
Multiplexing	NextSeq 550/Dx: 8 samples/run. NexSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples. NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples. NovaSeq X/X+: 1.5 B 32 samples, 10B 192 samples, 25B 480 samples.
Nucleic acid type	DNA, RNA
Sample throughput	8–960 samples per run
Specialized sample types	FFPE tissue
Species category	Human
Technology	Sequencing
Variant class	Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs), Tumor mutational burden (TMB), Genomic instability score (GIS), Microsatellite instability (MSI), Novel transcripts, Single nucleotide polymorphisms (SNPs), Structural variants

Required products

To run TruSight Oncology 500 v2 you need:

A TruSight Oncology 500 v2 library prep kit with a manual or automated workflow (note: DRAGEN variant calling pipeline included)
An index set:
- For manual workflows: Illumina DNA/RNA UD indexes Sets A-D
- For automated workflows: Illumina DNA/RNA UD indexes Set A-D v3, Auto
Reagent Kit if not included with library prep kit:
- NextSeq 550 or NextSeq 550Dx Reagent Kit v2.5 for high-output flow cell, 300 cycles
- NextSeq 1000 and 2000 XLEAP-SBS Reagent Kit with three flow cell options, 200 cycles
- NovaSeq 6000 or NovaSeq 6000Dx v1.5 Reagent Kit with four flow cell options, 200 cycles
- NovaSeq X Series Reagent Kit with three flow cell options, 200 cycles

To analyze with the DRAGEN variant calling pipeline you need:

A DRAGEN server for local analysis*, or
An Illumina Connected Analytics subscription with iCredits for cloud analysis

*NovaSeq 6000Dx paired DRAGEN server is enabled for TruSight Oncology 500 v2 local variant calling. All other instruments require a separate, stand-alone DRAGEN server for on-premise secondary analysis.

/ Results

Applications

TruSight Oncology 500 v2 enables comprehensive genomic profiling of FFPE tumor samples to assess a wide range of biomarkers using less sample, providing more informative results than single-gene or small panel assays.

Example workflow

Prep

TruSight Oncology 500 v2

Sequence

Analyze

Variant calling:

Insights and reporting:

Illumina Connected Insights

Velsera Clinical Genomics Workspace (CGW)

Project recommendations

Instrument	Recommended number of samples	Read length
NextSeq 500 System, NextSeq 550Dx in Research Mode	8 samples per run (high output flow cell), 100M paired-end reads, 3,500× coverage	2 × 101 bp
NextSeq 1000 System, NextSeq 2000 System	8 samples per run (P2 flow cell), 100M paired-end reads, 3,500× coverage 24 samples per run (P3 flow cell), 100M paired-end reads, 3,500× coverage 36 samples per run (P4 flow cell), 100M paired-end reads, 3,500× coverage	2 × 101 bp
NovaSeq X System	32 samples per run (1.5B flow cell), 100M paired-end reads, 3,500× coverage 192 samples per run (10B flow cell), 100M paired-end reads, 3,500× coverage 480 samples per run (25B flow cell), 100M paired-end reads, 3,500× coverage *Throughput shown is for the single flow cell NovaSeq X Instrument. NovaSeq X Plus offers a dual flow cell system with twice the capacity listed.	2 × 101 bp
NovaSeq 6000 System, NovaSeq 6000Dx in Research Mode	16 samples per run (SP flow cell), 100M paired-end reads, 3,500x coverage 32 samples per run (S1 flow cell), 100M paired-end reads, 3,500x coverage 72 samples per run (S2 flow cell), 100M paired-end reads, 3,500x coverage 192 samples per run (S4 flow cell), 100M paired-end reads, 3,500x coverage	2 × 101 bp

Related applications and methods

Pathology and clinical cancer research

Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.

Immuno-oncology research

Next-generation sequencing enables immuno-oncology researchers to gain insights into immunotherapy response factors and tumor immune evasion mechanisms.

Cancer sequencing methods

NGS-based cancer sequencing methods provide more information in less time compared to single-gene and array-based approaches.

Documentation

Product literature

Analysis of TMB and MSI Status with TruSight Oncology 500
Application note HTML externalFile

Input Recommendations for TMB, MSI, and Small Variant Analysis with TruSight Oncology 500
Technical note HTML externalFile

TruSight Oncology 500 Gene List - English
product_file EXCEL < 1 MB

TruSight Oncology 500 Gene List - Chinese (Simplified)
product_file EXCEL < 1 MB

- English
- Chinese (Simplified)
Product information sheet PDF < 1 MB

2 versions

- English
- Chinese (Simplified)
Product information sheet PDF < 1 MB

2 versions

- English
- Brazilian Portuguese
- French
- German
- Italian
- Spanish
Brochure PDF 6 MB

6 versions

Use of an alternative MIDI plate in the TruSight Oncology 500 workflow
Technical note PDF 11 MB

TruSight Oncology 500 v2
Data sheet PDF 3 MB

Support documentation

Compare

	TruSight Oncology 500 v2	TruSight Oncology 500 High-Throughput	TruSight Oncology 500 ctDNA v2
Assay time	3–4 days from sample input to final results	4–5 days from sample to results	3–4 days from purified nucleic acid to variant report
Automation capability	Liquid handling robot(s)	Liquid handling robot(s)	Liquid handling robot(s)
Automation details	Explore available automation methods	Explore available automation methods	Explore available automation methods
Cancer type	Pan-cancer, Solid tumor	Pan-cancer, Solid tumor	Pan-cancer, Solid tumor
Content specifications	Targeted sequencing of DNA from 523 genes and RNA from 55 genes for a total of 1.94 Mb panel size. MSI and TMB measurement included. The included HRD panel ^† includes coverage of ~25K SNPs to assess homologous recombination deficiency through a comprehensive genomic instability score (LOH+TAI+LST) powered by Myriad Genetics. ^†TruSight Oncology 500 v2 is not available for sale in Japan	Targeted sequencing of DNA from 523 genes of interest and RNA from 55 genes, for a total of 1.94 Mb panel size. MSI and TMB measurement included.	Immuno-oncology biomarker coverage: TMB and MSI Guideline coverage: Broad coverage of key guidelines for multiple solid tumor types Clinical trial coverage: Over 600 clinical trials (based on Velsera Knowledgebase, as of February 2023)
Description	Enables comprehensive genomic profiling of solid tumors using DNA and RNA from FFPE tissue. Provides variant detection and biomarker assessment from a single sample for broad tumor characterization.	A high-throughput comprehensive NGS assay to identify key biomarkers in guidelines and >1K clinical trials from a streamlined workflow using the NextSeq 1000 System, NextSeq 2000 System, NovaSeq 6000 System, NovaSeq 6000Dx instrument (in Research Mode), or NovaSeq X Series. Includes coverage of immuno-oncology biomarkers TMB and MSI.	Provides a noninvasive research method for comprehensive genomic profiling of liquid biopsy samples (ctDNA from blood plasma). This liquid biopsy approach provides insights about intra- and inter-tumor heterogeneity using a minimally invasive sample collection approach to complement tissue-based CGP.
Hands-on time	~7 hrs for manual workflow	~2.5 hr for automated workflow ~10.5 hr for manual workflow	~1.5 hr for automated workflow ~2.5 hr for manual workflow
Input quantity	30 ng DNA (as low as 10 ng), 40 ng RNA (as low as 20 ng)	40 ng DNA and/or 40–80 ng RNA	20 ng cfDNA (4 ml of plasma)
Instruments	NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System, NovaSeq X Plus System	NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System, NovaSeq X Plus System	NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System, NovaSeq X Plus System
Method	Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment	Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment	Targeted DNA sequencing, Target enrichment
Multiplexing	NextSeq 550/Dx: 8 samples/run. NexSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples. NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples. NovaSeq X/X+: 1.5 B 32 samples, 10B 192 samples, 25B 480 samples.	NextSeq 1000 and 2000: P2 flow cell 8 samples, P3 flow cell 24 samples, P4 flow cell 36 samples. NovaSeq 6000/Dx: SP flow cell 16 samples, S1 flow cell 32 samples, S2 flow cell 72 samples, S4 flow cell 192 samples. NovaSeq X Series*: 1.5B cartridge 32 samples, 10B cartridge 192 samples, 25B 480 samples.	NovaSeq X Series: 4 samples on 1.5B flow cell, 24 samples on 10B flow cell. NovaSeq 6000 System: 8 samples on S2 flow cell, 24 samples on S4 flow cell, 192 indexes maximum.
Nucleic acid type	DNA, RNA	DNA, RNA	DNA
Sample throughput	8–960 samples per run	NextSeq 1000 and 2000: 8–36 samples/run. NovaSeq 6000/Dx: 16-192 samples/run. NovaSeq X: 32–480 samples/run. NovaSeq X Plus: 32-960 samples/run.	4-48 samples/run
Specialized sample types	FFPE tissue	FFPE tissue	Circulating tumor DNA, Blood
Species category	Human	Human	Human
Technology	Sequencing	Sequencing	Sequencing
Variant class	Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs), Tumor mutational burden (TMB), Genomic instability score (GIS), Microsatellite instability (MSI), Novel transcripts, Single nucleotide polymorphisms (SNPs), Structural variants	Gene fusions, Somatic variants, Novel transcripts, Structural variants, Transcript variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)	Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)

Figures

Improved exon coverage across tissue types and input level

TruSight Oncology 500 v2 showed improved median exon coverage compared to the original TruSight Oncology 500 across 13 cancer types for 10–40 ng DNA input. Improved exon coverage was observed across most conditions, particularly for lower input levels.

**Increased coverage of the TERT promoter**

TruSight Oncology 500 v2 has improved coverage of GC-rich regions, such as the TERT promoter, which are often difficult to amplify by PCR.

Variant detection performance for key genes in FFPE samples

TruSight Oncology 500 v2 showed accurate detection of single nucleotide variants (SNVs), multinucleotide variants (MNVs), and indels across input levels and reagent lots.

CNV detection by gene

TruSight Oncology 500 v2 demonstrated reliable copy number variant (CNV) detection, as indicated by mean fold change. Consistent performance was observed for 10–40 ng DNA input.

Gene fusion and splice variant detection across RNA input levels

TruSight Oncology 500 v2 showed sensitive gene fusion and splice variant detection across 20–80 ng RNA input.

Concordance of TruSight Oncology 500 v2 genomic instability score (GIS) with a reference standard

GIS results from TruSight Oncology 500 v2 using 30 ng DNA input showed high concordance to a reference test across 102 FFPE tissue samples.

Subset of genomic tumor profiling biomarkers for multiple cancer types

Pan-cancer: BRAF, NTRK1, NTRK2, NTRK3, RET, MSI, TMB and HRD
Genes with biomarkers of significance^*								Genes with biomarkers of potential significance^†
Breast	BRCA1	BRCA2	ERBB2	ESR1	PALB2	PIK3CA		180
Colorectal	ERBB2	KRAS	NRAS					166
Bone	EGFR	ERG	ETV1	ETV4	EWSR1	FEV	FLI1	140
Bone	FUS	H3F3A	HEY1	IDH1	MDM2	NCOA2	SMARCB1	140
Lung	ALK	EGFR	ERBB2	KRAS	MET	NUTM1	ROS1	223
Melanoma	KIT	NRAS	ROS1					172
Ovarian	BRCA1	BRCA2	FOXL2					149
CNS^‡	APC	ATRX	CDKN2A	CDKN2B	EGFR	H3F3A	HIST1H3B	140
	HIST1H3C	IDH1	IDH2	MYCN	PTCH1	RELA	TERT
	TP53
Prostate	AR	ATM	BARD1	BRCA1	BRCA2	BRIP1	CDK12	151
	CHEK1	CHEK2	FANCL	FGFR2	FGFR3	PALB2	RAD51B
	RAD51C	RAD51D	RAD54L
Thyroid	HRAS	KRAS	NRAS	RET	TERT			165
Uterine & cervical	BRCA2	EPC1	ERBB2	ESR1	FOXO1	GREB1	JAZF1	138
	NCOA2	NCOA3	NUTM2A	NUTM2B	PAX3	PAX7	PHF1
	POLE	SMARCA4	SUZ12	TP53	YWHAE
Other solid tumors	ALK	APC	ARID1A	ASPSCR1	ATF1	ATIC	BAP1	152
	BCOR	BRCA1	BRCA2	CAMTA1	CARS	CCNB2	CDK4
	CDKN2A	CIC	CITED2	CLTC	COL1A1	COL6A3	CREB1
	CREB3L1	CREB3L2	CSF1	CTNNB1	DDIT3	DDX3X	DNAJB1
	DUX4	EED	EGFR	ERBB2	ERG	ETV1	ETV4
	ETV6	EWSR1	FEV	FGFR2	FGFR3	FLI1	FOXL2
	FOXO1	FOXO4	FUS	GLI1	HEY1	HGF	HMGA2
	IDH1	KRAS	LEUTX	MAML3	MDM2	MYB	MYOD1
	NAB2	NCOA2	NF1	NFATC2	NFIB	NR4A3	NRAS
	NUTMI	NUTM2A	NUTM2B	PALB2	PATZ1	PAX3	PAX7
	PDGFB	PDGFRA	PRKACA	PRKD1	RANBP2	ROS1	SDHA
	SDHB	SDHC	SDHD	SMARCB1	SS18	SSX1	SSX2
	SSX4	STAT6	SUZ12	TAF15	TCF12	TERT	TFE3
	TFEB	TFG	TP53	TPM3	TPM4	TRAF7	TSPAN31
	VGLL2	WT1	WWTR1	YAP1	YWHAE	ZC3H7B

The genes and biomarkers listed in this table are a subset of all genes included in the panel. See the TruSight Oncology 500 data sheet for the full gene list.

* Genes with biomarkers of significance linked to current drug labels or guidelines.

^†Genes with biomarkers of potential significance based on presence in clinical trials.

^‡CNS, central nervous system.

References:

1. Data calculations on file. Illumina, Inc. 2023.

Resources

Video resources

How TruSight Oncology 500 v2 is helping to advance cancer research at the Catalan Institute of Oncology

Learn how researchers at the Catalan Institute of Oncology are using TruSight Oncology 500 v2 to streamline their workflow and accelerate their research. In this video, scientists from the institute talk about the improved assay and share how faster results help to drive discovery.

Manual prep (18)

TruSight Oncology 500 v2 DNA/RNA Kit (24 samples)

20130527

Includes reagents for preparing and enriching up to 24 DNA and RNA samples. Purchase sequencing reagents and indexes separately.

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TruSight Oncology 500 v2 DNA Kit (48 samples)

20130528

Includes reagents for preparing and enriching up to 48 DNA samples. Purchase sequencing reagents and indexes separately.

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TruSight Oncology 500 v2 DNA/RNA Kit, For Use with NextSeq 550 (24 samples)

20130536

Includes reagents for preparing and enriching up to 24 DNA and RNA samples, and NextSeq 500/550 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA Kit, For Use with NextSeq 550 (48 samples)

20130537

Includes reagents for preparing and enriching up to 48 DNA samples, and NextSeq 500/550 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA/RNA Kit, For Use with NextSeq 1000/2000 P2 (24 samples)

20138676

Includes reagents for preparing and enriching up to 24 DNA and RNA samples, and NextSeq 1000/2000 P2 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA Kit, For Use with NextSeq 1000/2000 P2 (48 samples)

20138677

Includes reagents for preparing and enriching up to 48 DNA samples, and NextSeq 1000/2000 P2 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA/RNA Kit plus Velsera interpretation report (24 samples)

20138680

Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Velsera Clinical Genomics Workspace. Purchase sequencing reagents and indexes separately.

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TruSight Oncology 500 v2 DNA Kit plus Velsera interpretation report (48 samples)

20138681

Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Velsera Clinical Genomics Workspace. Purchase sequencing reagents and indexes separately.

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TruSight Oncology 500 v2 DNA/RNA Kit plus Velsera interpretation report, For Use with NextSeq 550 (24 samples)

20138686

Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Velsera Clinical Genomics Workspace, and NextSeq 500/550 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA Kit plus Velsera interpretation report, For Use with NextSeq 550 (48 samples)

20138687

Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Velsera Clinical Genomics Workspace, and NextSeq 500/550 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA/RNA Kit plus Velsera interpretation report, For Use with NextSeq 1000/2000 P2 (24 samples)

20138690

Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Velsera Clinical Genomics Workspace, and NextSeq 1000/2000 P2 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA Kit plus Velsera interpretation report, For Use with NextSeq 1000/2000 P2 (48 samples)

20138692

Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Velsera Clinical Genomics Workspace, and NextSeq 1000/2000 P2 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA/RNA Kit plus Illumina Connected Insights Software (24 samples)

20138695

Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Illumina Connected Insights. Purchase sequencing reagents and indexes separately.

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TruSight Oncology 500 v2 DNA Kit plus Illumina Connected Insights Software (48 samples)

20138696

Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Illumina Connected Insights. Purchase sequencing reagents and indexes separately.

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TruSight Oncology 500 v2 DNA/RNA Kit plus Illumina Connected Insights Software, For Use with NextSeq 550 (24 samples)

20138775

Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Illumina Connected Insights, and NextSeq 500/550 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA Kit plus Illumina Connected Insights Software, For Use with NextSeq 550 (48 samples)

20138776

Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Illumina Connected Insights, and NextSeq 500/550 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA/RNA Kit plus Illumina Connected Insights Software, For Use with NextSeq 1000/2000 P2 (24 samples)

20138779

Includes reagents for preparing and enriching up to 24 DNA and RNA samples and data interpretation reports using Illumina Connected Insights, and NextSeq 1000/2000 P2 sequencing reagents. Purchase indexes separately.

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TruSight Oncology 500 v2 DNA Kit plus Illumina Connected Insights Software, For Use with NextSeq 1000/2000 P2 (48 samples)

20138780

Includes reagents for preparing and enriching up to 48 DNA samples and data interpretation reports using Illumina Connected Insights, and NextSeq 1000/2000 P2 sequencing reagents. Purchase indexes separately.

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Index adapters (8)

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20091654

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20091656

Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20091658

Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20091660

Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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Illumina® DNA/RNA UD Indexes v3, Set A, Auto (96 Indexes 96 samples)

20141196

Includes one box of 96 Illumina DNA/RNA UD v3 Indexes Set A for labeling 96 samples sufficient for automation

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Illumina® DNA/RNA UD Indexes v3, Set B, Auto (96 Indexes 96 samples)

20141197

Includes one box of 96 Illumina DNA/RNA UD v3 Indexes Set B for labeling 96 samples sufficient for automation

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Illumina® DNA/RNA UD Indexes v3, Set C, Auto (96 Indexes 96 samples)

20141198

Includes one box of 96 Illumina DNA/RNA UD v3 Indexes Set C for labeling 96 samples sufficient for automation

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Illumina® DNA/RNA UD Indexes v3, Set D, Auto (96 Indexes 96 samples)

20141199

Includes one box of 96 Illumina DNA/RNA UD v3 Indexes Set D for labeling 96 samples sufficient for automation

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Accessory products (1)

TruSight FFPE QC Kit

20139070

TruSight™ FFPE QC Kit uses a real-time PCR assay to evaluate the quality of prospective DNA samples.

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Software and informatics options (13)

Illumina DRAGEN Server v4

20051343

Includes Advance Exchange support for the first year. Requires purchase of annual DRAGEN license.

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ICA Basic Annual Subscription

20044874

Illumina Connected Analytics (ICA) Basic Annual Subscription. This product includes 1 year of access to ICA Basic, including sequencing instrument connectivity, data management capabilities, and access to pre-packaged analysis tools.

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ICA Professional Annual Subscription

20044876

Illumina Connected Analytics (ICA) Professional Annual Subscription. This product includes 1 year of access to ICA, including sequencing instrument connectivity, data management capabilities, access to pre- packaged tools, and the ability to create customized workflows composed of tools, pipelines, data warehouses, and notebooks.

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ICA Enterprise Annual Subscription

20038994

Illumina Connected Analytics (ICA) Enterprise Annual Subscription. This product includes 1 year of access to ICA Enterprise, including sequencing instrument connectivity, data management capabilities, custom and pre-packaged analysis tools, and the Base module for data warehousing and mining. ICA Enterprise also includes optional HIPAA BAA (US-only), single sign-on (SSO), and a service level agreement (SLA).

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ICA Enterprise Srvc & Compliance Add-on

20066830

Illumina Connected Analytics (ICA) Compliance enables single sign-on (SSO), multi-factor authentication (MFA), HIPAA BAA (US-only), and a Service Level Agreement (SLA) for an ICA Basic Annual Subscription.

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Illumina Analytics - 1 iCredit

20042038