NET-Seq
ChIPmentation
ChIPmentation combines ChIP with sequencing library preparation by Tn5 transposase (tagmentation). Both ATAC-seq and ChIPmentation can be combined usefully in the same experiment to assay open chromatin and protein binding, respectively.1 This combination of methods takes advantage of the flexibility and efficiency provided by using Tn5 transposase in library preparation. Tagmentation is performed directly on bead-bound, immunoprecipitated chromatin, followed by standard library preparation methods.
Pros:
- Can generated accurate profiles from as little as 10,000 cells
- Simple, one-step reaction
Cons:
- None reported
- ChIPmentation: Schmidl C., Rendeiro A. F., Sheffield N. C. and Bock C. ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors. Nat Methods. 2015;12:963-965
- 1. Chaitankar V., Karakulah G., Ratnapriya R., Giuste F. O., Brooks M. J. and Swaroop A. Next generation sequencing technology and genomewide data analysis: Perspectives for retinal research. Prog Retin Eye Res. 2016;55:1-31