Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.
Assay time
Hands-on time
Input quantity
Illumina DNA Prep and Illumina DNA PCR-Free Prep are available. These library prep solutions offer improved performance while reducing turnaround time and hands-on time compared to TruSeq DNA Nano.
TruSeq DNA Nano enables efficient interrogation of samples with limited available DNA. Based on mechanical fragmentation–ligation chemistry, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.
TruSeq DNA Nano has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.
TruSeq DNA Nano offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.
TruSeq DNA Nano reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. Truseq DNA Nano products are validated for high-quality genomic coverage for virtually any next-generation sequencing application.
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.
| Assay time | ~6 hr total assay time |
|---|---|
| Automation capability | Liquid handling robot(s) |
| Automation details | Explore available automation methods |
| Description | A low-input research method that delivers high genome coverage quality and reduced bias. |
| Hands-on time | ~4 hr |
| Input quantity | 100 ng genomic DNA |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, MiSeqDx in Research Mode, MiniSeq System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System |
| Mechanism of action | Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. |
| Method | Genotyping by sequencing, Whole-genome sequencing, Shotgun sequencing |
| Multiplexing | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations |
| Nucleic acid type | DNA |
| Specialized sample types | Not FFPE-compatible, Low-input samples |
| Species category | Other, Mammalian, Mouse, Human, Rat, Plant |
| Species details | Compatible with most large DNA genomes. |
| Target insert size | 350 bp or 550 bp |
| Technology | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Chromosomal abnormalities, Germline variants, Structural variants, Insertions-deletions (indels), Copy number variants (CNVs) |
Illumina now offers modular product ordering to enable flexibility in your workflows. You may order any of the following library prep products with any of the following indexes.
Illumina Advantage large-scale sequencing products offer the highest level of service and support to ensure operational success. They feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater lab efficiency.
The Illumina Free Adapter Blocking Reagent is an optional enzymatic solution designed to block free adapters from library pools, to minimize the risk of index hopping.
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| NextSeq 550 System | 1 sample per run (based on 30× coverage of a human genome) |
350 bp insert: ≤ 2 × 100 bp |
Cancer whole-genome sequencing
Cancer whole-genome sequencing provides researchers with a base-by-base view of the unique mutations and abnormalities present in cancer tissue.
Large whole-genome sequencing informs disease research and population genomics studies and reveals disease-associated alleles.
Library prep and array kit selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
| TruSeq DNA Nano | TruSeq DNA PCR-Free | Illumina DNA Prep | |
|---|---|---|---|
| Assay time | ~6 hr total assay time | 5 hr total assay time | ~3-4 hr (from DNA extraction to normalized library) |
| Automation capability | Liquid handling robot(s) | Liquid handling robot(s) | Liquid handling robot(s) |
| Automation details | Explore available automation methods | Explore available automation methods | Explore available automation methods |
| Description | A low-input research method that delivers high genome coverage quality and reduced bias. | A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome. | A fast, flexible library prep workflow that accommodates an assortment of sample types and DNA input amounts, allowing access to a wide range of applications, from human to microbial whole-genome sequencing and more. |
| Hands-on time | ~4 hr | 4 hr | 1-1.5 hr |
| Input quantity | 100 ng genomic DNA | 1 ug DNA | 1-500 ng DNA for small genomes (e.g. microbial), or 100-500 ng DNA for large genomes (e.g. human). (For blood and saliva, see the reference guide). |
| Instruments | MiSeq System, NextSeq 550 System, NextSeq 2000 System, MiSeqDx in Research Mode, MiniSeq System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System | MiSeq System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System | MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000 System, NovaSeq X Plus System, MiSeq i100 Plus System, MiSeq i100 System |
| Mechanism of action | Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. | Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. | Bead-linked transposome |
| Method | Genotyping by sequencing, Whole-genome sequencing, Shotgun sequencing | Genotyping by sequencing, Whole-genome sequencing, Shotgun sequencing | Amplicon sequencing, Whole-genome sequencing, De novo sequencing, Shotgun sequencing |
| Multiplexing | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations | Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations |
| Nucleic acid type | DNA | DNA | DNA |
| Specialized sample types | Not FFPE-compatible, Low-input samples | Not FFPE-compatible | Blood, Not FFPE-compatible, Saliva |
| Species category | Other, Mammalian, Mouse, Human, Rat, Plant | Other, Mammalian, Mouse, Human, Rat, Plant | Drosophila, Any species, Mammalian, Mouse, Yeast, Zebrafish, Human, Rat, Plant, Virus, Nematode, Bacteria |
| Species details | Compatible with most large DNA genomes. | Compatible with most large DNA genomes. | Compatible with any species |
| Target insert size | 350 bp or 550 bp | 350 bp or 550 bp | ~350 bp |
| Technology | Sequencing | Sequencing | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Chromosomal abnormalities, Germline variants, Structural variants, Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Chromosomal abnormalities, Germline variants, Structural variants, Insertions-deletions (indels), Copy number variants (CNVs) | Single nucleotide polymorphisms (SNPs), Gene fusions, Loss of heterozygosity (LOH), Somatic variants, Chromosomal abnormalities, Germline variants, Structural variants, Insertions-deletions (indels), Copy number variants (CNVs) |
Determine the best library prep kit or array for your needs based on your starting material and method of interest.
TruSeq Nano DNA Low Throughput Library Prep Kit (24 samples)
20015964
Includes reagents and Sample Purification Beads for preparing 24 libraries. Purchase index adapters separately.
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TruSeq Nano DNA High Throughput Library Prep Kit (96 samples)
20015965
Includes reagents and Sample Purification Beads for preparing 24 libraries. Purchase index adapters separately.
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IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples)
20040870
Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Nano and TruSeq PCR-Free Library Prep Kits. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022370, with no changes to the remaining 184. Purchase library preps and probe panels separately.
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TG TruSeq® Nano DNA LT Sample Prep Kit, Set A (24 Samples)
TG-202-1001
The Kitted reagents for DNA Sample Preparation of 24 total samples for Single-Read, Paired-End, and Multiplex sequencing. This kit contains 12 unique single indexes.(Indexes 2,4,5,6,7,12,13,14,15,16,18,19)
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TG TruSeq® Nano DNA LT Sample Prep Kit, Set B (24 Samples)
TG-202-1002
The Kitted reagents for DNA Sample Preparation of 24 total samples for Single-Read, Paired-End, and Multiplex sequencing. This kit contains 12 unique single indexes. (Indexes 1,3,8,9,10,11,20,21,22,23,25,27)
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TG Truseq® Nano DNA Accessory Kit
TG-280-1001
Accessory kit for use with the TruSeq Nano DNA LT Kits A and B and cfDNA workflow. One Accessory kit provides enough addiitonal EPM reagent for 4 additional 16 sample batches through the cfDNA workflow.
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Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
NovaSeq 6000 Xp Workflow delivers the flexibility of independent lane loading and high library multiplexing per flow cell, maximizing sample throughput on a single run.
Offers a 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement.
Enable CGP with a pan-cancer panel capable of detecting key IO gene signatures (TMB, MSI) plus all main variant classes from ctDNA in blood plasma.
Reagent kits for the NovaSeq 6000 System consisting of ready-to-use pre-filled reagent cartridges and flow cells.
An efficient, fast, and integrated library preparation workflow that yields highly accurate data for sensitive sequencing applications.
Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.
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