HiSeq 3000/4000 SBS Kit

Includes sequencing reagents specifically designed for the HiSeq 3000/HiSeq 4000 Systems. Enables production-scale sequencing for diverse applications.Read More...
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HiSeq 3000/4000 SBS Kit (50 cycles)



HiSeq 3000/4000 SBS Kit (150 cycles)



HiSeq 3000/4000 SBS Kit (300 cycles)


Illumina Advantage Products

TG HiSeq® 3000/4000 SBS Kit (50 cycles)



TG HiSeq® 3000/4000 SBS Kit (150 cycles)



TG HiSeq® 3000/4000 PE ClusterKit



TG HiSeq® 3000/4000 SR ClusterKit



Product Highlights

HiSeq 3000/4000 SBS (Sequencing by Synthesis) Kits support a wide range of sequencing applications, making them ideal for high-throughput labs performing various genomic applications. Compatible with the HiSeq 3000 and HiSeq 4000 Sequencing Systems, the new kits leverage patterned flow cell technology for even cluster spacing and uniform feature size, to deliver extremely high cluster densities and significant increases in data output and daily throughput.

The HiSeq 3000/4000 SBS Kits enable up to 1500 Gb (1.5 Tb) of output per dual flow cell run when used with the HiSeq 3000/4000 PE Cluster Kit. This high capacity means that more samples can be sequenced simultaneously at a great depth, generating rich and meaningful data.

This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

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Product Literature

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Manuals and Support Information

HiSeq 3000/4000 SBS Kit Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

All HiSeq 3000/4000 SBS Kit Support 

Best Practices for Storing Sequencing Reagents

Sequencing reagent performance depends on thawing technique, thawed storage time, and storage conditions. Learn how to thaw and store sequencing reagents for optimal performance.

View Bulletin
Best Practices for Storing Sequencing Reagents

Related Products

cBot 2 System (discontinued)

The cBot 2 System streamlines the sequencing workflow by automating cluster generation. It is a required accessory instrument for HiSeq sequencers.

HiSeq 3000/4000 PE Cluster Kit

This kit enables cluster generation for subsequent sequencing on the HiSeq 3000 and HiSeq 4000 Systems.

HiSeq 3000/4000 SR Cluster Kit

Provides reagents for Single Read (SR) cluster generation on the cBot2 System. Cluster reagents are compatible with the HiSeq 3000 and HiSeq 4000 Systems.