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NextSeq 1000/2000 Reagents

NextSeq 1000/2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
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Reagents

NextSeq 1000/2000 P1 Reagents (100 cycles)

20074933

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NextSeq 1000/2000 P1 Reagents (300 Cycles)

20050264

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NextSeq™1000/2000 P1 Reagents(600cycles)

20075294

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NextSeq 1000/2000 P2 Reagents (100 Cycles) v3

20046811

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NextSeq 1000/2000 P2 Reagents (200 Cycles) v3

20046812

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NextSeq 1000/2000 P2 Reagents (300 Cycles) v3

20046813

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NextSeq™1000/2000 P2 300M Reagents(600cycles)

20075295

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NextSeq 2000 P3 Reagents (50 Cycles)

20046810

Price
 
 

NextSeq 2000 P3 Reagents (100 Cycles) 

20040559

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NextSeq 2000 P3 Reagents (200 Cycles)

20040560

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NextSeq 2000 P3 Reagents (300 Cycles)

20040561

Price
 
 
Accessory Products

NextSeq 1000/2000 Read and Index Primers

20046115

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NextSeq 1000/2000 Index Primer Kit

20046116

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NextSeq 1000/2000 Read Primer Kit

20046117

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PhiX Control v3

FC-110-3001

Price
 
 

NextSeq 1000/2000 Recycling Tool

20050631

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Cartridge and Flow Cell

Product Highlights

Multiple Flow Cell Configurations
  • Enables sequencing output of 10-360 Gb per run, providing adjustable output based on project needs
  • Increased flexibility to support new and emerging applications
Miniaturized Design Allows for Easy Storage, Use and Disposal
  • Minimal packaging and waste
  • Integrated and recyclable flow cell / reagent cartridge
Design Significantly Reduces the Cost and Complexity of Mid-throughput Sequencing
  • Enhanced output, lower operating costs, and increased ease of use
Browse the Data in BaseSpace Sequence Hub

The Illumina Public Data page can be found here.

NextSeq 2000: Illumina Exome - Illumina DNA Prep with Enrichment (Replicates of NA12878)
AmpliSeq for Illumina Comprehensive Cancer Panel (Replicates of NA12878)
NextSeq 2000: TruSeq Stranded mRNA (Replicates of UHR)

Access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Frequently Purchased Together

Specifications

Comparison Tables

Equivalent Performance for Exome Sequencing
  NextSeq 550 System NextSeq 2000 System Run 1 NextSeq 2000 System Run 2
Primary run metrics  
Paired-end reads PF 514.2M 518.3M 532.8M
Yield 103.9 Gb 103.7 Gb 106.5 Gb
Error rate 0.34% 0.32% 0.30%
Secondary analysis metrics  
Autosome callability 95.61% 95.95% 95.94%
% aligned reads 99.15% 99.08% 99.07%
Mean target coverage depth 78.05 77.85 78.15
Uniformity of coverage 93.20% 93.23% 93.28%
SNV precision 99.3% 99.3% 99.33%
SNV recall 94.1% 94.2% 94.27%
Indel precision 88.0% 89.3% 89.19%
Indel recall 83.3% 84.7% 84.75%
Equivalent Performance for Bulk mRNA Sequencing
  NextSeq 550 System NextSeq 2000 System Run 1 NextSeq 2000 System Run 2
Metric  
Paired-end reads PF 422.4M 511.6M 523.7M
Yield 63.6 Gb 76.76 Gb 78.25 Gb
Error rate 0.27% 0.27% 0.33%
Mean no. of genes detected (n = 24) 24,355 24,284 24,357

Supporting Data and Figures

Highly Concordant Results for mRNA-Seq

Quantification of specific RNA targets by bulk mRNA-Seq using the NextSeq 550 System are plotted on the y-axis. Results using the NextSeq 2000 System are plotted on the x-axis. Concordance of data is observed along the y = x trendline (R2 > 0.99 across four replicates).

concordant results figure

Performance Parameters for the NextSeq 1000 and 2000 Reagents

Read length NextSeq 1000/2000 P1 Reagents NextSeq 1000/2000 P2 Reagents NextSeq 2000 P3 Reagents
Output per flow cella
1 × 50 bp (P3 only)     60 Gb
2 × 50 bp 10 Gb 40 Gb 120 Gb
2 × 100 bp   80 Gb 240 Gb
2 × 150 bp 30 Gb 120 Gb 360 Gb
2 × 300 bp 60 Gb 180 Gb  
Reads CPF 100M 400M
(300M for 2 x 300 bp)		 1.2B
Quality scoresb
1 × 50 bp ≥ 90% of bases higher than Q30
2 × 50 bp ≥ 90% of bases higher than Q30
2 × 100 bp ≥ 85% of bases higher than Q30
2 × 150 bp ≥ 85% of bases higher than Q30
2 × 300 bp ≥ 80% of bases higher than Q30
Run time
1 × 50 bp     ~11 hours
2 × 50 bp ~10 hours ~13 hours ~19 hours
2 × 100 bp   ~21 hours ~33 hours
2 × 150 bp ~19 hours ~29 hours ~48 hours
2 × 300 bp ~34 hours ~44 hours  
  1. Output specifications based on a single flow cell using Illumina PhiX control library at supported cluster densities. CPF = clusters passing filter.
  2. Quality scores are based on using an Illumina PhiX control library. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors

Product Literature

NextSeq 1000 and NextSeq 2000 Systems FAQ

FAQ Document | PDF < 1 MB

Small whole-genome sequencing on NextSeq 1000 and NextSeq 2000 Systems

Application Note | PDF < 1 MB

16S rRNA sequencing on NextSeq™ 1000 and NextSeq 2000 Systems

Application Note | PDF < 1 MB

NextSeq 1000 and NextSeq 2000 single-cell RNA sequencing solution

Application Note | PDF < 1 MB

NextSeq 1000 and NextSeq 2000 RNA sequencing solution

Application Note | PDF < 1 MB

High-resolution, high-throughput spatial transcriptomics of complex tissues

Application Note | PDF 3 MB

NextSeq 1000 and NextSeq 2000 exome sequencing solution

Application Note | PDF < 1 MB

Explore the transcriptome with single-cell resolution

Technical Note | PDF 1 MB

Technical Note | PDF | 3 versions

Multiplex high-throughput proteomics with exceptional analytical specificity

Technical Note | PDF < 1 MB

Technical Note | PDF | 2 versions

Data Sheet | PDF | 5 versions

NextSeq 1000 and NextSeq 2000 Sequencing Systems Specification Sheet

Data Sheet | PDF < 1 MB

Manuals and Support Information

NextSeq 1000/2000 Sequencing System Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

All NextSeq 1000/2000 Sequencing System Support Resources  Support  

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