NextSeq 1000/2000 Reagent Kits now offer an extended shelf life of six months with improved Q30 scores.
NextSeq 1000/2000 Reagents
NextSeq 1000/2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
Reagents
NextSeq 1000/2000 P1 Reagents (100 cycles)
20074933
Price
Accessory Products
Total:
Product Highlights
Multiple Flow Cell Configurations
- Enables sequencing output of 10-360 Gb per run, providing adjustable output based on project needs
- Increased flexibility to support new and emerging applications
Miniaturized Design Allows for Easy Storage, Use and Disposal
- Minimal packaging and waste
- Integrated and recyclable flow cell / reagent cartridge
Design Significantly Reduces the Cost and Complexity of Mid-throughput Sequencing
- Enhanced output, lower operating costs, and increased ease of use
Browse the Data in BaseSpace Sequence Hub
The Illumina Public Data page can be found here.
NextSeq 2000: Illumina Exome - Illumina DNA Prep with Enrichment (Replicates of NA12878)
AmpliSeq for Illumina Comprehensive Cancer Panel (Replicates of NA12878)
NextSeq 2000: TruSeq Stranded mRNA (Replicates of UHR)
Access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub
Frequently Purchased Together
Specifications
Comparison Tables
Equivalent Performance for Exome Sequencing
| NextSeq 550 System | NextSeq 2000 System Run 1 | NextSeq 2000 System Run 2 | |
|---|---|---|---|
| Primary run metrics | |||
| Paired-end reads PF | 514.2M | 518.3M | 532.8M |
| Yield | 103.9 Gb | 103.7 Gb | 106.5 Gb |
| Error rate | 0.34% | 0.32% | 0.30% |
| Secondary analysis metrics | |||
| Autosome callability | 95.61% | 95.95% | 95.94% |
| % aligned reads | 99.15% | 99.08% | 99.07% |
| Mean target coverage depth | 78.05 | 77.85 | 78.15 |
| Uniformity of coverage | 93.20% | 93.23% | 93.28% |
| SNV precision | 99.3% | 99.3% | 99.33% |
| SNV recall | 94.1% | 94.2% | 94.27% |
| Indel precision | 88.0% | 89.3% | 89.19% |
| Indel recall | 83.3% | 84.7% | 84.75% |
Equivalent Performance for Bulk mRNA Sequencing
| NextSeq 550 System | NextSeq 2000 System Run 1 | NextSeq 2000 System Run 2 | |
|---|---|---|---|
| Metric | |||
| Paired-end reads PF | 422.4M | 511.6M | 523.7M |
| Yield | 63.6 Gb | 76.76 Gb | 78.25 Gb |
| Error rate | 0.27% | 0.27% | 0.33% |
| Mean no. of genes detected (n = 24) | 24,355 | 24,284 | 24,357 |
Supporting Data and Figures
Highly Concordant Results for mRNA-Seq
Quantification of specific RNA targets by bulk mRNA-Seq using the NextSeq 550 System are plotted on the y-axis. Results using the NextSeq 2000 System are plotted on the x-axis. Concordance of data is observed along the y = x trendline (R2 > 0.99 across four replicates).
Performance Parameters for the NextSeq 1000 and 2000 Reagents
| Read length | NextSeq 1000/2000 P1 Reagents | NextSeq 1000/2000 P2 Reagents | NextSeq 2000 P3 Reagents |
|---|---|---|---|
| Output per flow cella | |||
| 1 × 50 bp (P3 only) | 60 Gb | ||
| 2 × 50 bp | 10 Gb | 40 Gb | 120 Gb |
| 2 × 100 bp | 80 Gb | 240 Gb | |
| 2 × 150 bp | 30 Gb | 120 Gb | 360 Gb |
| 2 × 300 bp | 60 Gb | 180 Gb | |
| Reads CPF | 100M | 400M (300M for 2 x 300 bp) |
1.2B |
| Quality scoresb | |||
| 1 × 50 bp | ≥ 90% of bases higher than Q30 | ||
| 2 × 50 bp | ≥ 90% of bases higher than Q30 | ||
| 2 × 100 bp | ≥ 85% of bases higher than Q30 | ||
| 2 × 150 bp | ≥ 85% of bases higher than Q30 | ||
| 2 × 300 bp | ≥ 80% of bases higher than Q30 | ||
| Run time | |||
| 1 × 50 bp | ~11 hours | ||
| 2 × 50 bp | ~10 hours | ~13 hours | ~19 hours |
| 2 × 100 bp | ~21 hours | ~33 hours | |
| 2 × 150 bp | ~19 hours | ~29 hours | ~48 hours |
| 2 × 300 bp | ~34 hours | ~44 hours | |
- Output specifications based on a single flow cell using Illumina PhiX control library at supported cluster densities. CPF = clusters passing filter.
- Quality scores are based on using an Illumina PhiX control library. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors
Product Literature
NextSeq 1000 and NextSeq 2000 Systems FAQ
FAQ Document | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 single-cell RNA sequencing solution
Application Note | PDF < 1 MB
NextSeq 1000 and NextSeq 2000 RNA sequencing solution
Application Note | PDF < 1 MB
High-resolution, high-throughput spatial transcriptomics of complex tissues
Application Note | PDF 3 MB
NextSeq 1000 and NextSeq 2000 exome sequencing solution
Application Note | PDF < 1 MB
Small whole-genome sequencing on NextSeq 1000 and NextSeq 2000 Systems
Application Note | PDF < 1 MB
16S rRNA sequencing on NextSeq™ 1000 and NextSeq 2000 Systems
Application Note | PDF < 1 MB
Application Note | PDF < 1 MB
Explore the transcriptome with single-cell resolution
Technical Note | PDF 1 MB
Multiplex high-throughput proteomics with exceptional analytical specificity
Technical Note | PDF < 1 MB
Technical Note | PDF | 2 versions
Technical Note | PDF | 3 versions
Data Sheet | PDF | 5 versions
NextSeq 1000 and NextSeq 2000 Sequencing Systems specification sheet
Data Sheet | PDF < 1 MB
Manuals and Support Information
NextSeq 1000/2000 Sequencing System Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All NextSeq 1000/2000 Sequencing System Support Resources Support
NextSeq 1000/2000 Reagents
NextSeq 1000/2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
Illumina RNA Prep with Enrichment
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
Pan-Coronavirus Panel
This panel allows for characterization of at least 200 known coronavirus genera for surveillance of animal reservoirs and sequencing of known coronaviruses.
TruSeq RNA Exome
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
Viral Surveillance Panel
Viral Surveillance Panel offers whole-genome sequencing and characterization of 66 viruses including SARS-CoV-2, influenza, and polio with target enrichment.