Reagents for the NextSeq 1000/2000 System feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.
NextSeq 1000/2000 reagents leverage XLEAP-SBS chemistry to enable a wide range of applications. These reagents offer:
Easy-to-use reagent cartridges deliver a streamlined workflow that reduces the chance for errors, lowers operating costs, improves recyclability, and minimizes waste. The fully-integrated cartridges include reagents, fluidics, and the waste holder, streamlining library loading and instrument use.
Simply prepare the reagent cartridge, insert the flow cell, load the library into the cartridge, and insert into the instrument.
| Instruments | NextSeq 2000 System, NextSeq 1000 System |
|---|---|
| Nucleic acid type | DNA, RNA |
| Reagent type | Paired-end sequencing, Cluster generation, Sequencing by synthesis, Single-read sequencing |
| Technology | Sequencing |
Use XLEAP-SBS P1 reagents on both the NextSeq 1000 and NextSeq 2000 Systems. Maximum output: 60 Gb. Maximum single-end reads per run: 100M.
Use XLEAP-SBS P2 reagents on both the NextSeq 1000 and NextSeq 2000 Systems. Maximum output: 240 Gb. Maximum single-end reads per run: 400M.
Use XLEAP-SBS P3 reagents on the NextSeq 2000 System only. Maximum output: 360 Gb. Maximum single-end reads per run: 1.2B.
Use XLEAP-SBS P4 reagents on the NextSeq 2000 System only. Maximum output: 540 Gb. Maximum single-end reads per run: 1.8B.
The NextSeq 1000/2000 Index Primer Kit, Read Primer Kit, and Read and Index Primers are optional but required when both custom sequencing primers and Illumina primers are used in a run.*
PhiX Control v3 is recommended for all runs, but is optional for well-balanced library types.
*For Illumina DNA PCR-Free Prep, the XLEAP-SBS cartridge already contains the primers, whereas the standard SBS cartridge does not.
NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (600 Cycles)
20100981
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 600 cycles of sequencing, plus dual-indexing support (up to 100M single reads).
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NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (300 Cycles)
20100982
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 100M single reads).
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NextSeq™ 1000/2000 P1 XLEAP-SBS™ Reagent Kit (100 Cycles)
20100983
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 100M single reads).
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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (600 Cycles)
20100984
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 600 cycles of sequencing, plus dual-indexing support (up to 400M single reads).
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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (300 Cycles)
20100985
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 400M single reads).
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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100986
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 400M single reads).
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NextSeq™ 1000/2000 P2 XLEAP-SBS™ Reagent Kit (100 Cycles)
20100987
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 1000/2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 400M single reads).
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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (300 Cycles)
20100988
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).
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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100989
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).
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NextSeq™ 2000 P3 XLEAP-SBS™ Reagent Kit (100 Cycles)
20100990
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 1.2B single reads).
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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (300 Cycles)
20100992
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 300 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).
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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (200 Cycles)
20100993
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 200 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).
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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (100 Cycles)
20100994
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 100 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).
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NextSeq™ 2000 P4 XLEAP-SBS™ Reagent Kit (50 Cycles)
20100995
Powered by XLEAP-SBS chemistry, provides kitted NextSeq 2000 reagents for 50 cycles of sequencing, plus dual-indexing support (up to 1.8B single reads).
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NextSeq™ 1000/2000 XLEAP-SBS™ Read and Index Primer Kit
20112856
Reagents to utilize custom read and index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge and MiSeq i100 series cartridges. This kit is sufficient for 1 custom read primer and 1 custom index primer use
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NextSeq™ 1000/2000 XLEAP-SBS™ Index Primer Kit
20112858
Reagents to utilize custom index primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge and MiSeq i100 series cartridges. This kit is sufficient for 10 custom index primer uses.
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NextSeq™ 1000/2000 XLEAP-SBS™ Read Primer Kit
20112859
Reagents to utilize custom read primers with the NextSeq 1000/2000 XLEAP-SBS™ cartridge and MiSeq i100 series cartridges. This kit is sufficient for 10 custom read primer uses.
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NextSeq™1000/2000 P1 Reagents(100cycles)
20074933
Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (100 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 1000/2000 P1 Reagents (300 Cycles)
20050264
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (300 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq™1000/2000 P1 Reagents(600cycles)
20075294
Provides kitted reagents for 600 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (600 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 1000/2000 P2 Reagents (100 Cycles) v3
20046811
Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (100 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20.
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NextSeq 1000/2000 P2 Reagents (200 Cycles) v3
20046812
Provides kitted reagents for 200 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (200 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20.
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NextSeq 1000/2000 P2 Reagents (300 Cycles) v3
20046813
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 400M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (300 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 2000 P3 Reagents (50 Cycles)
20046810
Provides kitted reagents for 50 cycles of sequencing, plus dual-indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (50 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 2000 P3 Reagents (100 Cycles)
20040559
Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (100 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 2000 P3 Reagents (200 Cycles)
20040560
Provides kitted reagents for 200 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (200 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 2000 P3 Reagents (300 Cycles)
20040561
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support (up to 1.1B single reads). Includes: NextSeq 2000 Reagent Cartridge (300 cycles), NextSeq 2000 P3 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq™1000/2000 P2 300M Reagents(600cycles)
20075295
Provides kitted reagents for 600 cycles of sequencing, plus dual- indexing support (up to 300M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (600 cycles), NextSeq 1000/2000 P2 Flow Cell, and RSB with Tween 20. This reagent kit is based on the v3 reagents.
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NextSeq 1000/2000 Read and Index Primers
20046115
Reagents to utilize custom read and index primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 1 custom read primer and 1 custom index primer use.
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NextSeq 1000/2000 Index Primer Kit
20046116
Reagents to utilize custom index primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 10 custom index primer uses.
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NextSeq 1000/2000 Read Primer Kit
20046117
Reagents to utilize custom read primers with the NextSeq 1000/2000 cartridge. This kit is sufficient for 10 custom read primer uses.
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PhiX Control v3
FC-110-3001
Kitted DNA control for the Illumina sequencing platform. Compatible with Single and Paired End reads up to 150 base pairs. (10ul of 10nM template solution)
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NextSeq 1000/2000 Recycling Tool
20050631
Tool used to break down NextSeq 1000/2000 cartridge into individual components for easy recycling
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A variety of applications can be run on the NextSeq 2000 System. Each application will have different run times. Run times can range from 8–44 hr.
See the NextSeq 2000 specifications for more information.
The NextSeq 2000 System can generate 1.8B single-end reads with P4 reagents.
See the NextSeq 2000 specifications for more information.
For maximum flexibility, the NextSeq 2000 System offers four flow cell types.
See the NextSeq 2000 specifications for more information.
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