Reagent kits for the NovaSeq X Series include a reagent cartridge, buffer cartridge, flow cell, lyo insert, pre-load buffer, and library tube strip.
For NovaSeq X Series 1.5B Reagent Kits, strictly adhere to prescribed thaw conditions and timings in the System Guide. Do not rely solely on visual checks to confirm reagents have thawed. For NovaSeq X Series 100c and 200c 25B Reagent Kits, ensure you have updated to instrument software v1.3 or newer.
NovaSeq X Series Reagent Kits combine the power of XLEAP-SBS chemistry with flexible, efficient, and sustainable workflows to enable transformational science at scale.
Improvements in XLEAP-SBS reagents allow for shipping and storage in lyophilized form, minimizing environmental impact for significant reductions in packaging size, weight, plastic mass, and waste.
NovaSeq X Series Reagent Kits are powered by XLEAP-SBS chemistry: a faster, more accurate, and robust update to traditional sequencing by synthesis (SBS) chemistry that supports consistent, reliable data generation for results you can trust.
*Compared to NovaSeq 6000 onboard workflow
†Compared to NovaSeq 6000 Reagent Kits
| Instruments | NovaSeq X System, NovaSeq X Plus System |
|---|---|
| Maximum output |
16,000 Gb (per dual 25B* flow cell run on the NovaSeq X Plus system) 8000 Gb (per single 25B* flow cell run on the NovaSeq X system) |
| Maximum reads per run |
52 billion single reads (per dual 25B* flow cell run on the NovaSeq X Plus system) 26 billion single reads (per single 25B* flow cell run on the NovaSeq X system) |
| Nucleic acid type | DNA, RNA |
| Reagent type | Paired-end sequencing, Cluster generation, Sequencing by synthesis |
| Technology | Sequencing |
Each reagent kit contains the required products for a single flow cell sequencing run. For sequencing runs requiring custom primers, Custom-Primer Buffer is available for purchase (sold separately).
Spare accessories including air filters, wash flow cells, wash reagent cartridges, and sample tubes are also available for purchase (sold separately).
A dedicated support section is not currently available for this product
NovaSeq™ X Series 25B Reagent Kit (300 Cyc)
20104706
This reagent kit provides one NovaSeq X Series 25B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series 25B Reagent Kit (200 Cyc)
20125968
This reagent kit provides one NovaSeq X Series 25B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems. Please note that the lyo insert provided with this kit is compatible only with NovaSeq X 25B 100/200c kits 9MN 20125967 and 20125968) and not compatible with NovaSeq X Series 25B Reagent Kit 300c (MN 20104705).
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NovaSeq™ X Series 25B Reagent Kit (100 Cyc)
20125967
This reagent kit provides one NovaSeq X Series 25B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 100 cycles run on the NovaSeq X and NovaSeq X Plus systems. Please note that the lyo insert provided with this kit is compatible only with NovaSeq X 25B 100/200c kits 9MN 20125967 and 20125968) and not compatible with NovaSeq X Series 25B Reagent Kit 300c (MN 20104705).
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NovaSeq X Series 10B Reagent Kit (300 Cycle)
20085594
This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq X Series 10B Reagent Kit (200 Cycle)
20085595
This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq X Series 10B Reagent Kit (100 Cycle)
20085596
This reagent kit provides one NovaSeq X Series 10B flow cell (with 8- lanes) and reagent consumables to support a single flow cell 100 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series 1.5B Reagent Kit (300 Cyc)
20104705
This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) and reagent consumables to support a single flow cell 300 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series 1.5B Reagent Kit (200 Cyc)
20104704
This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) and reagent consumables to support a single flow cell 200 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series 1.5B Reagent Kit (100 Cyc)
20104703
This reagent kit provides one NovaSeq X Series 1.5B flow cell (with 2- lanes) with reagent consumables to support a single flow cell 100 cycles run on the NovaSeq X and NovaSeq X Plus systems.
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NovaSeq™ X Series Custom-Primer Buffer
20100055
Buffer used for the custom primer workflow on the NovaSeq X Series. This kit is sufficient for 1 custom primer workflow for a single flow cell sequencing run.
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NOVASEQ™X Series Wash Flow Cell
20088067
NOVASEQ™X Series Wash Flow Cell is used during washes on the NovaSeq X or NovaSeq X Plus system. This part contains one wash flow cell.
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NOVASEQ™X Series Air Filter
20073109
Replacement NovaSeq X Series Air Filter for the NovaSeq X or NovaSeq X Plus system. This part contains one air filter.
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NOVASEQ™X Series Library Tube Strip (1.5B)
20072272
NOVASEQ™ X Series Library Tube Strip compatible with a NovaSeq X Series 1.5B Reagent Kit. This part contains one 1.5B (2-lane) library tube strip.
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NOVASEQ™X Series Library Tube Strip (10B/25B)
20101913
NOVASEQ™X Series Library Tube Strip compatible with a NovaSeq X Series 10B Reagent Kit or a NovaSeq X Series 25B Reagent Kit. This part contains one 10B/25B (8-lane) library tube strip.
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