DamID allows the identification of protein-binding sites in living cells without the need for crosslinking or immunoprecipitation. It was developed in 2006 as a microarray method before it was adapted to NGS. DamID involves the low-level expression of a fusion protein consisting of DNA adenine methyltransferase (Dam) and a chromatin protein of interest. This fusion protein is targeted to the native binding sites of the chromatin protein, where Dam methylates adenines in the surrounding DNA. Subsequently, the methylated DNA fragments are isolated, amplified by selective PCR, and sequenced.