scBS-Seq
scBS-Seq
Single-cell bisulfite sequencing (scBS-seq) is a version of the well established bisulfite sequencing (BS-seq) and post-bisulfite adaptor tagging (PBAT) protocols, modified to detect methylated cytosines in genomic DNA from single cells. In this method, after single cells are isolated, genomic DNA is treated with sodium bisulfite, which fragments the DNA. The converted DNA, then undergoes random priming several times and is PCR amplified for sequencing. Deep sequencing provides high resolution single nucleotide resolution of methylated cytosines from single cells.