DNA methylation analysis can help researchers gain valuable insight into gene regulation and identify potential biomarkers. Aberrant DNA methylation has been implicated in many disease processes, including cancer, obesity, and addiction. DNA methylation is also a common subject of agrigenomic investigations into responses to drought, temperature extremes, and other environmental changes.
High-throughput technologies such as next-generation sequencing (NGS) and microarrays enable genome-wide methylation profiling studies. These technologies offer new ways to understand the significance of DNA methylation, providing novel insights into the functional consequences of variation.
Methylation Sequencing with NGS
NGS enables comprehensive profiling of methylation patterns at single-base resolution across the whole genome, or in targeted epigenetic regions of interest.
Arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample.
|Most important to me||Comprehensive methylome coverage||High throughput (large sample numbers)|
|Least important to me||Throughput||Coverage|
~36 million CpGs (whole genome)
~3.3. million CpGs (targeted)
All (whole genome)
|Learn More About Methylation Sequencing||Learn More About Methylation Arrays|
Methylation analysis and RNA-Seq help researchers understand how endurance training makes epigenetic changes to the human genome.Read Interview
Targeted methylation sequencing identifies epigenetic signatures that correlate with brain image scans of substance abusers.Read Interview
Studies of epigenetic alterations in cancer, such as aberrant methylation and transcription factor binding, can provide insight into important tumorigenic pathways. Learn more about cancer epigenetics research.
Genome-wide DNA methylation analysis can help researchers understand the functional mechanisms at work in complex diseases such as Alzheimer’s disease or asthma. Learn more about complex disease research.