Understanding the role of methylation in regulating gene expression

DNA Methylation Analysis

DNA methylation analysis can help researchers gain valuable insight into gene regulation and identify potential biomarkers. Aberrant DNA methylation has been implicated in many disease processes, including cancer, obesity, and addiction. DNA methylation is also a common subject of agrigenomic investigations into responses to drought, temperature extremes, and other environmental changes.

High-throughput technologies such as next-generation sequencing (NGS) and microarrays enable genome-wide methylation profiling studies. These technologies offer new ways to understand the significance of DNA methylation, providing novel insights into the functional consequences of variation.

 
Methylation Sequencing with NGS

NGS enables comprehensive profiling of methylation patterns at single-base resolution across the whole genome, or in targeted epigenetic regions of interest.

Methylation Microarrays

Arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample.

Most important to me Comprehensive methylome coverage High throughput (large sample numbers)
Least important to me Throughput Coverage
#CpGs covered

~36 million CpGs (whole genome)

~3.3. million CpGs (targeted)

~850,000 CpGs
Species

All (whole genome)

Human (targeted)

Human
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Field Guide to Methylation Methods

An overview of the impact of cytosine methylation and the primary methods used to detect cytosine modifications.

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Field Guide to Methylation Methods
Epigenetic Patterns of Exercise

Methylation analysis and RNA-Seq help researchers understand how endurance training makes epigenetic changes to the human genome.

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Epigenetic Contributions to Addiction

Targeted methylation sequencing identifies epigenetic signatures that correlate with brain image scans of substance abusers.

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Methylation and Metastasis

Researchers investigate the epigenetics behind cancer metastasis using DNA methylation microarrays and NGS.

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Epigenetic Changes in Cancer
Epigenetic Changes in Cancer

Studies of epigenetic alterations in cancer, such as aberrant methylation and transcription factor binding, can provide insight into important tumorigenic pathways. Learn more about cancer epigenetics research.

Methylation Profiling for Complex Disease
Gene Target Identifcation in Complex Diseases

Methylation provides insight into why genes may be expressed at a given level. Epigenetic processes have established links to various complex diseases, including cancers, autoimmune disorders, neurological disorders, and psychiatric disorders. Learn more about gene target identifcation in complex disease research.

Chromatin Accessibility Analysis
Chromatin Accessibility Analysisr

ATAC-Seq does not require prior knowledge of regulatory elements, making it a powerful epigenetic discovery tool. It is a popular method for determining chromatin accessibility across the genome. Learn more about ATAC-Seq.

Sequencing Method Explorer
Sequencing Method Explorer

Use this interactive tool to explore experimental next-generation sequencing (NGS) library preparation methods compiled from the scientific literature. Find a method.

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When Whole-Genome Sequencing Is Not Enough
When Whole-Genome Sequencing Is Not Enough

Researchers explore cancer subtypes using Illumina technology for whole-exome, whole-genome, and whole-epigenome sequencing.

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Epigenome Roadmap
Epigenome Roadmap

A collection of research papers that systematically characterize epigenomic landscapes in primary human tissues and cells.

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Epigenome Blueprint
Epigenome Blueprint

A consortium generating 100 reference epigenomes to understand gene activation and repression in healthy and diseased human cells.

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DNA Methylation Analysis
Targeted Methylation Analysis

TruSeq Methyl Capture EPIC rapidly produces target enrichment-based bisulfite sequencing libraries from human DNA samples.

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