Features > 285k markers across the methylome for high-resolution epigenetic analyses of diverse murine strains.
Number of samples
Number of markers
The Infinium Mouse Methylation BeadChip Kit offers a unique combination of comprehensive, expert-selected coverage and high sample throughput.
The Infinium Mouse Methylation BeadChip provides balanced coverage of CpG islands, translation start sites, enhancers, imprinted loci, and other regions. Infinium HD chemistry enables content selection independent of bias limitations often associated with methylated DNA capture methods.
The Infinium Mouse Methylation BeadChip achieves > 98% reproducibility for technical replicates and shows high correlation with whole-genome bisulfite sequencing data.
Enables epigenome-wide association studies, xenograft experiments, preclinical research, and more.
| Assay type | Infinium HD Methylation |
|---|---|
| Automation capability | Automated array loader |
| Input quantity | 250 ng DNA |
| Instruments | iScan System |
| Method | Methylation array |
| Nucleic acid type | DNA |
| Number of markers | Fixed markers: 285k |
| Number of samples | 12 samples per array |
| Sample type details | May be used with fresh/frozen tissue. Analyze changes in DNA methylation induced by exogenous chemicals, including DAC. |
| Specialized sample types | Blood |
| Species category | Mouse |
| Technology | Microarray |
| Variant class | Differentially methylated cytosines |
Choose the Infinium Mouse Methylation BeadChip kit configuration that offers the right sample throughput for your needs.
The Infinium Mouse Methylation BeadChip powers a broad spectrum of murine epigenetic analysis studies, including epigenome-wide association studies, preclinical research, xenograft studies, and more.
Infinium Mouse Methylation BeadChip
Genome-wide methylation profiling studies with microarray and NGS technologies can provide novel insights into the functional impact of methylation.
Illumina offers solutions for epigenetic analysis. Use our tools to study epigenetic modifications and their impact on gene regulation.
Learn how to profile gene expression changes for a deeper understanding of biology.
The array uses both Infinium I and Infinium II assays. Infinium I assay design employs two bead types per CpG locus, 1 each for the methylated and unmethylated states. The Infinium II design uses 1 bead type, with the methylated state determined at the single base extension step after hybridization.
GenomeStudio Software supports DNA methylation analysis on any platform. Data are displayed in intuitive graphics such as heatmaps.
| Infinium Mouse Methylation BeadChip | Infinium MethylationEPIC v2.0 Kit | |
|---|---|---|
| Assay type | Infinium HD Methylation | Infinium HD Methylation |
| Automation capability | Automated array loader | Automated array loader |
| Input quantity | 250 ng DNA | 250 ng DNA |
| Instruments | iScan System | NextSeq 550 System, NextSeq 550Dx in Research Mode, iScan System |
| Method | Methylation array | Methylation array |
| Nucleic acid type | DNA | DNA |
| Number of markers | Fixed markers: 285k | ~ 930k |
| Number of samples | 12 samples per array | 8 samples per array |
| Sample type details | May be used with fresh/frozen tissue. Analyze changes in DNA methylation induced by exogenous chemicals, including DAC. | |
| Specialized sample types | Blood | Blood, FFPE tissue |
| Species category | Mouse | Human |
| Technology | Microarray | Microarray |
| Variant class | Differentially methylated cytosines | Differentially methylated cytosines |
Library Prep and Array Kit Selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
Dr. Wanding Zhou presents an overview of this powerful tool for mouse researchers.
Methylation array data analysis tips
Experts discuss their strategies for analyzing Illumina methylation array data.
Infinium Mouse Methylation BeadChip Kit (24 samples)
20041558
Includes two, 12-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 24 DNA samples. Requires ≥ 250 ng DNA for input. Each kit is processed as a single batch.
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Infinium Mouse Methylation BeadChip Kit (48 samples)
20041559
Includes four, 12-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 DNA samples. Requires ≥ 250 ng DNA for input. Each kit is processed as a single batch.
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Infinium Mouse Methylation BeadChip Kit (96 samples)
20041560
Includes eight, 12-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 96 DNA samples. Requires ≥ 250 ng DNA for input. Each kit is processed as a single batch.
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Infinium Mouse Methylation275k-12+ v1.0 Kit (24 Samples)
20041561
Includes two, 12-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 24 DNA samples. Customize content with an additional 3K–10K markers using DesignStudio or select from a list of add-on content available as consortia boosters. Requires ≥ 250 ng DNA for input. Each kit is processed as a single batch.
Infinium Mouse Methylation 275k-12+ v1.0 Kit (48 Samples)
20041562
Includes four, 12-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 DNA samples. Customize content with an additional 3K–10K markers using DesignStudio or select from a list of add-on content available as consortia boosters. Requires ≥ 250 ng DNA for input. Each kit is processed as a single batch.
Infinium Mouse Methylation275k-12+ v1.0 Kit (96 Samples)
20041563
Includes eight, 12-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 96 DNA samples. Customize content with an additional 3K–10K markers using DesignStudio or select from a list of add-on content available as consortia boosters. Requires ≥ 250 ng DNA for input. Each kit is processed as a single batch.
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The GenomeStudio Methylation Module extracts beta values and provides clustering for Infinium Mouse Methylation BeadChip data. GenomeStudio controls normalization with the Mouse Methylation BeadChip is not supported at this time. Also, the BeadArray Controls Reporter software tool is not supported at this time.
There are many freeware applications in Bioconductor, such as SeSAMe, that provide enhanced normalization and visualization options. Illumina does not provide support for these freeware solutions directly.
Illumina has not validated the array for 5-hMc. However, publications have used the Infinium HumanMethylation450K and MethylationEPIC BeadChips for 5-hMc analysis and it is possible that this protocol will work on the Infinium Mouse Methylation BeadChip.
For more information, see Nazor KL, Boland MJ, Bibikova M, et al. Application of a low cost array-based technique—TAB-Array—for quantifying and mapping both 5mC and 5hmC at single base resolution in human pluripotent stem cells. Genomics. 2014;104(5):358-367. doi: 10.1016/j.ygeno.2014.08.014
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Robust methylation profiling microarray providing extensive coverage of CpG islands, genes, and enhancers. Ideal for genetic and rare disease research, cancer research, and classification.
Evaluate sample quality and repair degraded FFPE DNA samples for use in Infinium array-based assays.
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