AmpliSeq for Illumina

    • AmpliSeq for Illumina On-Demand Panels

    Select from a catalog of > 5000 pretested genes to create custom targeted resequencing panels

    Scientists reviewing Ampliseq on computer screen

    Design DNA or RNA panels with pretested gene content

    Confidently tailor panel designs for human disease research by selecting from over 5000 genes relevant for inherited disease research, including hereditary cancer, primary immunodeficiency, hearing loss, muscular dystrophy, and more. All genes have been pretested on the NextSeq 550 Sequencing System.

    Use the free web-based DesignStudio Assay Design Tool to add up to 500 genes of interest and order your panel in convenient small packs of 24 or 96 reactions.

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    Featured AmpliSeq for Illumina products

    AmpliSeq for Illumina On-Demand

    Tailor panel designs for human disease research efficiently and conveniently by selecting from a catalog of over 5,000 pretested genes.

    Key features and benefits

    Simplified panel creation

    With the simple user interface, robust algorithms, and dynamic feedback provided by DesignStudio, On-Demand Panels can be created easily.

    Pretested content

    Fuel your study with optimized and tested content in numerous genetic disease research areas.

    Content flexibility

    Design panels with 1–500 genes and 24–15,000 amplicons.

    Streamlined data analysis

    Perform secondary analysis workflows in the cloud via BaseSpace Sequence Hub or on-instrument via Local Run Manager.

    Low-input sample types

    Obtain high-quality data with high coverage uniformity even with minimal input from challenging sample types like formalin-fixed, paraffin-embedded (FFPE) tissue.

    Fast library prep

    Prepare an on-target, high-uniformity amplified library in as little as 5 hours with just 1.5 hours of hands-on time.

    • Data sheet PDF < 1 MB 2 versions

    How AmpliSeq for Illumina On-Demand Panels work

    To create your AmpliSeq for Illumina On-Demand Panel, use DesignStudio Assay Design Tool to select parameters like assay type, species, sample type, and maximum amplicon length. Add your targets using genes or coordinates of interests. Receive, review, and adjust your design before ordering your custom PCR-based library prep assay.

    Libraries are prepared using multiplexed PCR amplification of your targets prior to sequencing on an Illumina next-generation sequencing (NGS) system using sequencing by synthesis (SBS) chemistry. You can analyze data on the cloud with the DRAGEN Amplicon pipeline or on-instrument with Local Run Manager to get accurate results without extensive bioinformatics resources.

    Learn more about DesignStudio Assay Design Tool
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    Introducing AmpliSeq for Illumina

    Learn about the robust performance of AmpliSeq for Illumina from a wide variety of sample types.

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    AmpliSeq for Illumina On-Demand Panel workflow

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    Select content

    User-friendly assay design:

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    Prep
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    Sequence
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    Analyze

    Custom assay design services

    Improve coverage and enable add-on or non-human species designs with Illumina Concierge design support. Our Concierge team provides end-to-end project management, in-silico coverage, and design assistance.

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    Research design team in collaboration

    AmpliSeq for Illumina On-Demand Panel FAQ

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    Additional resources

    Amplicon sequencing

    Perform ultra-deep sequencing of PCR amplicons with cost-effective analysis of hundreds of target genomic regions in one assay.

    Targeted resequencing

    Select and sequence specific genes or genomic regions of interest using predesigned or custom designed panels for gene profiling studies.

    Speak to a specialist

    Contact an Illumina specialist to learn more about AmpliSeq for Illumina products.