AmpliSeq for Illumina RNA Library Prep Training
|AmpliSeq for Illumina Custom RNA Panel||TruSeq Targeted RNA Expression Library Prep Kits|
|Assay Time||5.5-7.5 hours||1 day|
|Hands-On Time||<1.5 hours||4 hours|
|Input Quantity||1 ng||50 ng RNA, 50 ng high-quality total RNA, ≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality|
|Mechanism of Action||Multiplex PCR||Amplification|
|Method||Amplicon Sequencing , Custom Sequencing, Targeted RNA Sequencing||Targeted RNA Sequencing|
|Multiplexing||Up to 384-plex||Up to 384 samples per sequencing run|
|Nucleic Acid Type||RNA||RNA|
|Specialized Sample Types||Blood, FFPE Tissue||FFPE Tissue, Low-Input Samples|
|Species Category||Human||Human, Mouse, Rat|
|System Compatibility||iSeq 100 , MiniSeq , MiSeq , MiSeq, NextSeq 1000 , NextSeq 2000 , NextSeq 500 , NextSeq 550||MiniSeq , MiSeq , MiSeq, NextSeq 2000 , NextSeq 500 , NextSeq 550|
|Variant Class||Transcript Variants||Gene Fusions, Single Nucleotide Polymorphisms (SNPs), Transcript Variants|
Libraries were prepared from 10 ng of Universal Human RNA using an AmpliSeq for Illumina Custom RNA Panel (208 amplicon panel size) and sequenced on the MiSeq System. Results show a high concordance (R2 = 0.997) between the two replicates of the same RNA sample. R2 is a statistical measurement of data correlation.
The AmpliSeq for Illumina Custom RNA Panel enables researches to analyze differential gene expression across genes of interest. To demonstrate assay performance, Human Brain Reference RNA and Universal Human RNA were analyzed using 5 AmpliSeq for Illumina Custom RNA panels of varying size (number of amplicons). Libraries were sequenced on a MiSeq System.