Create custom targeted resequencing panels optimized for your research goals
Create custom panels to focus on your specific genes and targets of interest when they are not available on ready-to-use panels. Use the free web-based DesignStudio Assay Design Tool to submit your target regions of interest and get personalized panel content for your study.
Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.
Targeted custom RNA research panels optimized for sequencing up to 1200 genomic targets of interest.
Customizable targeted panel for detecting fusion genes and gene expression profiling of up to 12–1200 targets of interest.
To create your AmpliSeq for Illumina Custom Panel, use DesignStudio Assay Design Tool to select parameters like assay type, species, sample type, and maximum amplicon length. Add your targets using genes or coordinates of interests. Targets can be selected based on preloaded reference genomes, or you can upload a custom reference genome.
To design your amplicons, an optimized algorithm considers a range of factors including GC content, specificity, and coverage. Candidate amplicons are visualized and assessed using estimated success scores. You can filter, add, or remove amplicons from your design. After visualization and QC, you can order your panel.
Learn about the robust performance of AmpliSeq for Illumina from a wide variety of sample types.
User-friendly assay design:
AmpliSeq for Illumina custom panel options:
Improve coverage and enable add-on or non-human species designs with Illumina Concierge design support. Our Concierge team provides end-to-end project management, in-silico coverage, and design assistance.
Sign up for or log in to DesignStudio Assay Design Tool to start a new design. Select a preloaded reference genome, then add in your genomic regions of interest. If you like the content from AmpliSeq for Illumina Ready-to-Use or Community Panels, you can directly upload Product Manifest Files into the DesignStudio tool as a starting point for further customization. Learn more about .
You can pool up to 96 samples per sequencing run with integrated sample barcodes. The actual number of samples that can be pooled depends on the number of amplicons and the desired depth of coverage. DesignStudio Assay Design Tool features an online calculator to inform these parameters.
You can view BaseSpace Sequence Hub demo data sets for AmpliSeq for Illumina Custom RNA Panels and other AmpliSeq for Illumina Panels in BaseSpace Public Data.
No, although DesignStudio Assay Design Tool returns high confidence amplicon designs that have delivered unprecedented amplicon multiplexing performance, performance of each design will need to be tested empirically as design and sample input can vary. To create a custom panel from a catalog of > 5K pretested genes, use AmpliSeq for Illumina On-Demand Panels.
The includes a step-by-step guide to using the DesignStudio Assay Design Tool. Contact Illumina Technical Support if you need further assistance with DesignStudio. If you need help with ordering, contact a sales representative.
Perform ultra-deep sequencing of PCR amplicons with cost-effective analysis of hundreds of target genomic regions in one assay.
Perform targeted cancer sequencing with a focused gene panel, helping reduce costs and complete studies quickly.
Select and sequence specific genes or genomic regions of interest using predesigned or custom designed panels for gene profiling studies.
Contact an Illumina specialist to learn more about AmpliSeq for Illumina products.
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