Create amplicon panels with the genes you want. AmpliSeq for Illumina On Demand panels provide a choice of > 5000 pretested genes with known content relevant for inherited disease research, including hereditary cancer, primary immunodeficiency, hearing loss, and muscular dystrophy.
AmpliSeq chemistry and Illumina next-generation sequencing (NGS) technology provide unmatched data quality, even from low-quality starting materials such as formalin-fixed, paraffin-embedded (FFPE) tissues.
|AmpliSeq for Illumina On-Demand||AmpliSeq for Illumina Custom DNA Panel||Illumina DNA Prep with Enrichment|
|Content Specifications||Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes||Custom content of interest - up to 5 Mb||Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
|Description||A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.||A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy.||A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.|
|Input Quantity||1-100 ng DNA||1–100 ng (10 ng recommended per pool)||10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).|
|Mechanism of Action||Multiplex PCR||Multiplex PCR||Bead-bound transposomes and hybrid-capture chemistry|
|Method||Amplicon Sequencing, Targeted DNA Sequencing||Amplicon Sequencing, Custom Sequencing, Genotyping by Sequencing, Targeted DNA Sequencing||Custom Sequencing, Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing|
|Multiplexing||Up to 96-plex||Up to 96-plex||Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.|
|Number of Reactions||24 or 96 reactions||750 or 3000 reactions, 750 or 3000 reactions||Custom workflow: 16 or 96 samples|
|Specialized Sample Types||Blood, FFPE Tissue||Blood, FFPE Tissue||Blood, FFPE Tissue, Saliva|
|Species Category||Human||Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato||Human, Other|
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