Order the NovaSeq X Series
Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
Tailor panel designs for human disease research efficiently and conveniently by selecting from a catalog of over 5,000 pretested genes.
Assay time
Hands-on time
Input quantity
Create amplicon panels with the genes you want. AmpliSeq for Illumina On Demand panels provide a choice of > 5000 pretested genes with known content relevant for inherited disease research, including hereditary cancer, primary immunodeficiency, hearing loss, and muscular dystrophy.
AmpliSeq chemistry and Illumina next-generation sequencing (NGS) technology provide unmatched data quality, even from low-quality starting materials such as formalin-fixed, paraffin-embedded (FFPE) tissues.
Choice of > 5000 pretested genes
Assay time | As low as 5 hr (library prep only; doesn't include library quantification, normalization, or pooling time) |
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Content specifications | Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes |
Description | A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. |
Hands-on time | 1.5 hr |
Input quantity | 1-100 ng DNA |
Instruments | MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NextSeq 500 System |
Mechanism of action | Multiplex PCR |
Method | Targeted DNA sequencing, Amplicon sequencing |
Multiplexing | Up to 96-plex |
Nucleic acid type | DNA |
Number of reactions | 24 or 96 reactions |
Specialized sample types | Blood, FFPE tissue |
Species category | Human |
Technology | Sequencing |
Variant class | Single nucleotide polymorphisms (SNPs), Germline variants, Insertions-deletions (indels) |
In addition to an AmpliSeq for Illumina On-Demand Panel, you'll need AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina.
AmpliSeq for Illumina Direct FFPE DNA is strongly recommended for FFPE samples. The AmpliSeq for Illumina Sample ID Panel is an optional accessory to facilitate sample management.
AmpliSeq for Illumina On-Demand
AmpliSeq for Illumina CD Indexes
AmpliSeq for Illumina On-Demand Panels
Create amplicon panels with the genes you want. Select from a catalog of pretested genes with known content relevant for inherited disease research.
Our sequencing and microarray technologies support a broad range of cancer genomics research applications, from DNA to RNA analysis, epigenetics, and more.
Literature is not currently available for this product.
AmpliSeq for Illumina On-Demand | AmpliSeq for Illumina Custom DNA Panel | Illumina DNA Prep with Enrichment | |||
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Assay time | As low as 5 hr (library prep only; doesn't include library quantification, normalization, or pooling time) | As low as 5 hr (library prep only; doesn't include library quantification, normalization, or pooling time) | ~6.5 hr | ||
Content specifications | Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes | Custom content of interest - up to 5 Mb |
Custom: 0.5 - 15 Mb genomic content of interest, with Illumina Custom Enrichment Panels and Illumina Custom Enrichment Panel v2 Fixed panels: Content varies by panel. |
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Description | A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. | A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. | Prepare sequencing libraries for a variety of experiments, including whole-exome, custom, and fixed panel targeted sequencing, from low DNA inputs and a variety of sample types in ~6.5 hours. | ||
Hands-on time | 1.5 hr | 1.5 hours | ~2 hr, ~2 hr | ||
Input quantity | 1-100 ng DNA | 1–100 ng (10 ng recommended per pool) | 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). | ||
Instruments | MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NextSeq 500 System | MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode | MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System, NovaSeq X Plus System | ||
Mechanism of action | Multiplex PCR | Multiplex PCR | Bead-bound transposomes and hybrid-capture chemistry | ||
Method | Targeted DNA sequencing, Amplicon sequencing | Custom sequencing, Targeted DNA sequencing, Amplicon sequencing, Genotyping by sequencing | Custom sequencing, Targeted DNA sequencing, Exome sequencing, Target enrichment | ||
Multiplexing | Up to 96-plex | Up to 96-plex | Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. | ||
Nucleic acid type | DNA | DNA | DNA | ||
Number of reactions | 24 or 96 reactions | 750 or 3000 reactions | Custom workflow: 16 or 96 samples | ||
Specialized sample types | Blood, FFPE tissue | Blood, FFPE tissue | Blood, Low-input samples, FFPE tissue, Saliva | ||
Species category | Human | Any species, Tomato, Bovine, Mouse, Canine, Rice, Ovine, Hamster, Chicken, Porcine, Human, Maize, Soybean | Other, Human | ||
Technology | Sequencing | Sequencing | Sequencing | ||
Variant class | Single nucleotide polymorphisms (SNPs), Germline variants, Insertions-deletions (indels) | Single nucleotide polymorphisms (SNPs), Loss of heterozygosity (LOH), Somatic variants, Germline variants, Insertions-deletions (indels), Copy number variants (CNVs) |
AmpliSeq™ for Illumina® On-Demand Panel (24 Reactions, 1-50 Genes)
20023977
Sufficient for 24 reactions and 1–50 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.
AmpliSeq™ for Illumina® On-Demand Panel (24 Reactions, 51-300 Genes)
20023983
Sufficient for 24 reactions and 51–300 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.
AmpliSeq™ for Illumina® On-Demand Panel (24 Reactions, 301-500 Genes)
20023978
Sufficient for 24 reactions and 301–500 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.
AmpliSeq™ for Illumina® On-Demand Panel (96 Reactions, 1-50 Genes)
20023979
Sufficient for 96 reactions and 1–50 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.
AmpliSeq™ for Illumina® On-Demand Panel (96 Reactions, 51-300 Genes)
20023980
Sufficient for 96 reactions and 51–300 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.
AmpliSeq™ for Illumina® On-Demand Panel (96 Reactions, 301-500 Genes)
20023981
Sufficient for 96 reactions and 301–500 genes. Purchase AmpliSeq Library PLUS for Illumina and AmpliSeq CD Indexes Set A for Illumina separately.
AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ for Illumina® Sample ID Panel
20019162
Includes eight single nucleotide polymorphism (SNP)-targeting primer pairs and one gender discriminating primer pair sufficient for 96 reactions when paired with AmpliSeq Library PLUS for Illumina. Enables quick and accurate sample identification. Purchase library prep, probe panels, and index adapters separately.
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AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.
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