AmpliSeq for Illumina Custom DNA Panel

Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.Read More...
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Panel

AmpliSeq™ Custom DNA Panel for Illumina® 

20020495

AmpliSeq™ Custom DNA Large Panel for Illumina®

20020497


Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ CD Indexes Set A for Illumina® (96 Indexes, 96 Samples)

20019105

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Accessory Products

AmpliSeq™ for Illumina® Sample ID Panel

20019162

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

Accurate Data Across Various Sample Types

Achieve unmatched data quality with AmpliSeq chemistry and Illumina next-generation sequencing (NGS) technology, even from low-quality starting materials such as formalin-fixed, paraffin-embedded (FFPE) tissues.

Include only the genes and targets of interest
  • Easily design custom content with DesignStudio, a free, user-friendly, online tool that creates panels optimized for the targets or specific genomic content of interest.
  • Flexible and tunable designs from 12 to over 12,000 amplicons, and multiple amplicon size options.
Fast, Simple Workflow
  • Increased efficiency with a 5.5-hour library preparation workflow with only 1.5 hours of hands-on time.
Scalable Solution
  • Multiple options for sequencing platforms, data analysis, and support meet virtually any throughput requirements.

Learn More About AmpliSeq for Illumina

Specifications

Product Comparison

AmpliSeq for Illumina Custom DNA Panel AmpliSeq for Illumina On-Demand Nextera Flex for Enrichment Nextera Rapid Capture Custom Enrichment Kit
Content Specifications Custom content of interest - up to 5 Mb Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
Custom genomic content of interest (0.5 - 15 Mb)
Description A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. A custom research workflow that utilizes custom panels, existing Illumina designed panels or previously ordered custom projects to enable enrichment of targeted genomic content. 
Input Quantity 1–100 ng (10 ng recommended per pool) 1-100 ng DNA 10-1000 ng high-quality genomic DNA or ≥ 50 ng FFPE DNA. (For blood and saliva, see the reference guide). 50 ng DNA
Mechanism of Action Multiplex PCR Multiplex PCR Enrichment-compatible, bead-linked transposome Enzymatic fragmentation and hybridization-based enrichment
Method Amplicon Sequencing , Custom Sequencing, Genotyping by Sequencing , Targeted DNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing Custom Sequencing, Exome Sequencing , Target Enrichment , Target Enrichment, Targeted DNA Sequencing Custom Sequencing, Target Enrichment , Target Enrichment, Targeted DNA Sequencing
Multiplexing Up to 96-plex Up to 96-plex Up to 96 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. 12-plex (pool up to 12 samples per enrichment reaction)
Number of Reactions 750 or 3000 reactions 24 or 96 reactions Custom workflow: 16 or 96 samples 48, 96, or 288 samples
Specialized Sample Types Blood, FFPE Tissue FFPE Tissue Blood, FFPE Tissue, Saliva Not FFPE-Compatible
Species Category Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato Human Human, Other Human

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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