Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
MiSeq Reagent Kits v3 enable the highest output of all MiSeq kits. The v3 kits retain the same pre-filled, ready-to-use reagent cartridges as the v1 and v2 kits, but offer improvements to enable increased cluster density and read length, as well as higher Q30 scores. The v3 kits offer:
Updated system software unlocks the full benefits of the v3 kits. Access the latest software.
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.
| Instruments | MiSeq System, MiSeqDx in Research Mode |
|---|---|
| Maximum output | 15 Gb (600-cycle), 3.8 Gb (150-cycle) |
| Maximum reads per run | Up to 25M |
| Nucleic acid type | DNA, RNA |
| Reagent type | Paired-end sequencing, Cluster generation, Sequencing by synthesis |
| Technology | Sequencing |
A demonstration of the steps to prepare the MiSeq Reagent Cartridge prior to sequencing.
Learn the steps to handle and clean the MiSeq flow cell prior to sequencing.
Sequencing Coverage Calculator
Calculator to help determine the reagents and sequencing runs needed to arrive at desired coverage for your experiment.
MiSeq Reagent Kit v3 (150-cycle)
MS-102-3001
Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 150-cycle run on the MiSeq System.
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MiSeq Reagent Kit v3 (600-cycle)
MS-102-3003
Provides kitted reagents for up to 625 cycles of sequencing on the MiSeq System. Includes: Paired-End Reagent plate (600-cycles), MiSeq Flow Cell, and Wash Buffer.
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TG MiSeq Reagent Kit v3 (600 cycle)
TG-142-3003
Provides kitted reagents for up to 625 cycles of sequencing on the MiSeq System. Includes: Paired-End Reagent plate (600-cycles), MiSeq Flow Cell, and Wash Buffer.
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TG MiSeq® Reagent Kit v3 (150 cycle)
TG-142-3001
Provides kitted reagents for up to 175 cycles of sequencing on the MiSeq System. Includes: Paired-End Reagent plate (150-cycles), MiSeq Flow Cell, and Wash Buffer.
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A MiSeq flow cell is a single lane flow cell that can support up to 25M reads per run.
There are two new reagents in MiSeq Reagent Kit v3, the Incorporation Mix (IMT) and Scan Mix (USM). In addition, the PR2 volume has been increased to 500 ml to support longer runs. This ensures double the output of a single run using the v3 kits (3.3–15 Gb) compared to v2 kits (0.75–8.5 Gb).
The number of samples that can be loaded depends on the expected coverage and genome size.
For assistance, use the Sequencing Coverage Calculator tool.
Yes. As with MiSeq v2 kits, MiSeq v3 kits are compatible with all Illumina library prep kits. Some library prep protocols require slight changes to reach optimum clusters. Illumina recommends a starting concentration of 4 nM to begin the denature and dilute step before loading libraries for sequencing.
Read lengths of 2 × 75 bp can take ~21 hr, while read lengths of 2 × 300 bp can take ~56 hr.
If you currently own a MiSeq System, you may be eligible for a promotion on one of the following instruments: MiSeq i100 Plus System, NextSeq 1000 System, or NextSeq 2000 System*. Experience a simpler workflow with dry instrumentation, easier run set up, expanded output, and faster secondary analysis. Complete this form to contact a sales representative and see if you are eligible.
* These promotional programs are available for a limited time. Terms and conditions apply.
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