|MiniSeq System High-Output Kit*||MiniSeq Rapid Kit*||MiniSeq System Mid-Output Kit*|
|Read Length||2 × 150 bp||2 × 75 bp||1 × 75 bp||1 × 100 bp||2 × 150 bp|
|Total Time†||~24 hrs||13 hrs||7 hrs||< 5 hrs||17 hrs|
|Output||6.6–7.5 Gb||3.3–3.75 Gb||1.65–1.875 Gb||2 Gb||2.1–2.4 Gb|
* Install specifications based on Illumina PhiX control library at
supported cluster densities (between 129 and 165 k/mm2 clusters
passing filter). Actual performance parameters may vary based on sample type,
sample quality, and clusters passing filter. All MiniSeq kits are paired-end
† Total times include cluster generation, sequencing with High Output flow cell, and base calling with quality scores on a MiniSeq System.
|MiniSeq System High-Output Kit||MiniSeq System Rapid Kit||MiniSeq System Mid-Output Kit|
|Single Reads||22–25 million||20 million||7–8 million|
|Paired-End Reads||44–50 million||N/A||14–16 million|
|MiniSeq System High-Output Kit||MiniSeq System Mid-Output Kit||MiniSeq System Rapid Kit|
|> 80% bases higher than Q30 at 2 × 150 bp||> 80% bases higher than Q30 at 2 × 150 bp||> 85% bases higher than Q30 at 1 × 100 bp|
|> 85% bases higher than Q30 at 2 × 75 bp|
|> 85% bases higher than Q30 at 1 × 75 bp|
†† A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
The power of proven Illumina sequencing, now more accessible than ever.View Specification Sheet
The MiniSeq System harnesses proven Illumina SBS technology to deliver highly accurate data and robust performance for a broad range of applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
2-channel SBS chemistry offers efficient sequencing and data generation. 2-channel SBS reduces cycle and data processing times, while delivering the same high quality and accuracy that sets Illumina systems apart. This innovation makes NGS an affordable solution for use as an everyday tool in laboratories worldwide.
Access cost-efficient sequencing with high data quality, even for low numbers of samples. MiniSeq Rapid reagents are now available and reduce sequencing run times to < 5 hours, enabling faster detection of respiratory viruses and pathogens, including SARS-CoV-2.Order Now
There is a MiniSeq Software System Suite upgrade available. Complete the form to schedule an upgrade and receive several enhancements including a Windows 10 operating system upgrade and feature updates to Local Run Manager Software.