Simplest workflow, fastest results
Perform a wide range of applications on our fastest, simplest benchtop sequencer, with flexible output and read-to-use workflows optimized for novice users
Take the guesswork out of your next workflow. The NGS Workflow Finder provides recommendations and resources so you can sequence with confidence.
Whether you use our predesigned supported workflows or build your own, we have options to support every step of the workflow.
Supported sample-to-analysis workflows: The MiSeq i100 and MiSeq i100 Plus Systems offer supported sample-to-analysis workflows for various methods. These workflows include library prep kits, panels, sequencing on the MiSeq i100 Series, DRAGEN secondary analysis, and data interpretation (where applicable). Additional workflows will be supported in the future.
Targeted, culture-free sequencing of complex biological samples to identify and compare bacterial taxa present in microbial communities.
Supported sample-to-analysis workflow
Culture-free NGS method that identifies microbes in complex samples. A cost-effective, culture-free method for identification of bacterial strains not found using traditional methods.
Resources
Functional characterization of all microbial genomes present in a complex sample. Fast, integrated library prep completes in < 4 hr with minimal hands-on time.
Resources
Functional quantification of all coding and noncoding RNAs present in complex samples. Efficient depletion of ribosomal RNA and human globin mRNA across multiple species and sample types.
Resources
Microbial sequencing and analysis of a single, known organism, whether from an isolated, cultured bacterial colony or a primary sample being surveilled for a determined pathogen of interest.
Supported sample-to-analysis workflow
Comprehensive method for mapping, finishing, or comparing microbial genomes for deep insights. Fast, integrated library prep completes in < 4 hr with minimal hands-on time.
DRAGEN sWGS onboard MiSeq i100
Resources
Targeted NGS panel for identification of Mycobacterium tuberculosis complex strains. Prepare sequencing libraries from as little as 9 pg of DNA.
Panel + library prep
Supported sample-to-analysis workflow
Comprehensive, amplicon-based assay for characterization of the SARS-CoV-2 virus. Low- to-mid-throughput NGS assay enables labs of any size to surveil novel SARS-CoV-2 variants.
Panel
Library prep
Resources
Supported sample-to-analysis workflow
Multiplexed, amplicon-based assay for WGS of influenza A/B viruses for public health surveillance and research studies. Automation-friendly RT-PCR and tagmentation-based workflow for influenza A/B analyses.
Panel + library prep
Supported sample-to-analysis workflow
Custom, user-defined targeted sequencing for identification and characterization of specific microbes of interest. Compatible with custom, published, or commercially available primer sets targeting microbes of interest.
Multipathogen sequencing via amplicon-based NGS involves analyzing genomic regions of interest with ultradeep sequencing of PCR amplicons. Enables full characterization of viruses.
Supported sample-to-analysis workflow
Obtain WGS data for characterization of a broad range of viruses, including SARS-CoV-2, influenza, and poliovirus. Perform concurrent analysis of RNA and DNA viruses compatible with a range of sample types.
Panel + kit
DRAGEN Microbial Enrichment Plus onboard MiSeq i100
Resources
Targeted, streamlined detection of common respiratory viruses, such as SARS-CoV-2 and influenza A/B. Target enrichment through hybrid–capture methods provides highly sensitive detection without the need for high read depth.
Panel + kit
DRAGEN Microbial Enrichment Plus onboard MiSeq i100
Supported sample-to-analysis workflow
NGS-based panel that targets > 280 respiratory pathogens and > 2000 antimicrobial resistance markers with streamlined, sample-to-results workflow.
Panel + kit
DRAGEN Microbial Enrichment Plus onboard MiSeq i100
Supported sample-to-analysis workflow
NGS-based panel that targets > 170 uropathogens and > 3700 antimicrobial resistance markers with streamlined, sample-to-results workflow.
Panel + kit
DRAGEN Microbial Enrichment Plus onboard MiSeq i100
Targeted cancer sequencing, comprising a focused selection of genes associated with specific cancer types or pathways, complement broader genomic profiling approaches and offer users multiple options to interrogate and understand cancer biology.
Supported sample-to-analysis workflow
Targeted detection of multiple variants, including CNVs and gene fusions. Single-tube SLIMamp workflow provides fast library prep and turnaround time.
Library prep
SLIMamp Amplicon Prep
Supported sample-to-analysis workflow
Targeted interrogation of 58 genes of interest highly relevant to myeloid cancers. Single-tube SLIMamp workflow provides fast library prep and turnaround time.
Library prep
SLIMamp Amplicon Prep
Resources
Supported sample-to-analysis workflow
Focused detection of multiple gene rearrangement regions of interest across solid tumor types. Single-tube SLIMamp workflow provides fast library prep and turnaround time.
Panel
Pillar oncoReveal Multi-Cancer RNA Fusion v2 Panel
Library prep
SLIMamp Amplicon Prep
Comprehensive assessment of 1385 cancer-related RNA transcripts. In-depth and cost-effective assessment of cancer-related transcripts across most sample types, including FFPE samples.
Panel
Library prep
SLIMamp Amplicon Prep
Resources
Supported sample-to-analysis workflow
Targeted sequencing panel for BRCA1/2 mutations and copy number variations. Single-tube SLIMamp workflow provides fast library prep and turnaround time.
Library prep
SLIMamp Amplicon Prep
Data analysis
Data interpretation
Supported sample-to-analysis workflow
Targeted sequencing panel that assesses germline mutations across 113 genes and 125 SNPs associated with genetic cancer risk predispostion. Expert-defined content developed with input from key opinion leaders.
Library prep
Data analysis
Data interpretation
Supported sample-to-analysis workflow
Analyze B- or T-cell receptor diversity and clonal expansion to gain insights into the adaptive immune system. Flexible library prep from a wide range of RNA input amounts from as little as 10 ng up to 3 μg of RNA.
Takara Data Analysis
Watch the unveiling of the MiSeq i100 Series in this webinar. Discover how it will accelerate insights and discoveries with faster, simpler sequencing.
Experience the quality, precision, and ease of use in every step with our library prep portfolio advancements.
MiSeq i100 Series training
Get high-quality results on Illumina technology even faster with instructor-led or hands-on courses and web-based training options.
Looking for more? Reach out to talk to a specialist about the MiSeq i100 Series or explore a variety of events introducing the MiSeq i100 Series, from roadshows in cities around the world to webinars and more.
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