Illumina DRAGEN secondary analysis enables labs of all sizes and disciplines to maximize the value of the genome. It provides powerful, intuitive analysis onboard or in the cloud, a broad range of applications, and an intuitive, ready-to-use set of pipelines.
MiSeq i100 software and informatics
User-friendly data analysis, onboard or in the cloud
Simplified data analysis with optimized software to reduce reliance on informatics experts
Your email address is never shared with third parties.
Comprehensive, accurate, and efficient
Built for intuitive analysis
DRAGEN secondary analysis is built with high standards of data privacy and protection and minimizes the need for bioinformatics expertise.
DRAGEN analysis included
Onboard DRAGEN secondary analysis is included with purchase of a MiSeq i100 or MiSeq i100 Plus System; no internet connectivity is required.
Fewer touchpoints
4× reduction in manual touchpoints for cloud analysis compared to the MiSeq System.
Easier data management
Easier data management and data transfer with 5× lossless compression of FASTQ files.
Faster results
Results in as little as two hours for onboard workflows for most applications.
Ready-to-use analysis, where you need it
DRAGEN secondary analysis onboard the MiSeq i100 Series delivers rapid, ultra-efficient, and easy-to-use analysis, overcoming data bottlenecks and reducing reliance on informatics experts.
In addition to onboard pipelines, data from the MiSeq i100 Series can be streamed into BaseSpace Sequence Hub, a user-friendly genomics cloud-computing environment offering simplified run setup, monitoring, and analysis. There, users can access a broad suite of DRAGEN pipelines for accurate secondary analysis and visualization of NGS data to generate meaningful biological results.
For labs interested in scalability and custom solutions, data can be streamed from the MiSeq i100 Series to Illumina Connected Analytics, a flexible cloud bioinformatics platform that supports a broader range of pipelines and highly configurable, scalable analysis.
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Generate exceptionally accurate results with DRAGEN secondary analysis
DRAGEN secondary analysis offers award-winning variant calling accuracy. Multigenome (graph) reference and Illumina machine learning in DRAGEN enable exceptional accuracy using PrecisionFDA benchmarking data.1
DRAGEN data analysis tools
DRAGEN apps are available onboard the MiSeq i100 Series or within BaseSpace Sequence Hub, simplifying data analysis.
Analyzes DNA from amplicon sequencing of prokaryotic 16S small subunit rRNA genes; provides visuals of taxonomic classification.
Performs mapping, alignment, and germline or somatic variant calling for DNA or RNA amplicon data.
Converts the native output format of Illumina sequencing systems (BCL) to sample separated FASTQ files using hardware acceleration on the DRAGEN platform. Available onboard.
Performs germline and somatic variant calling on whole-exome and targeted panel NGS data.
Calculates common metrics used for quality control of high-throughput sequencing data. Available onboard.
Provides taxonomic classification for complex microbial samples, including single sample and aggregate reporting.
Analyzes viral whole genomes from tiled amplicon NGS panels.
DRAGEN Microbial Enrichment Plus
Generates consensus sequences for viral identification from enrichment-based NGS panels and detection of pathogenic microbes and/or antimicrobial resistance markers. Available onboard.
DRAGEN Small WGS
Performs somatic variant calling for whole-genome sequencing data. Available onboard.
Takara SMARTer Human TCR a/b Profiling
Analyze B- or T-cell receptor diversity and clonal expansion to gain insights into the adaptive immune system.
Comprehensive solutions
DRAGEN data analysis apps are part of sample-to-analysis NGS workflows available for a wide variety of applications.
Additional software
The Illumina Connected Software portfolio is a comprehensive software suite offering solutions for every workflow stage, from sample intake to insights discovery.
Track samples, optimize procedures and workflows, and automate routine tasks with a highly customizable laboratory information management system (LIMS).
Intuitive cloud-based run set up and monitoring plus a broad range of bioinformatics apps for simplified data analysis.
Operationalize bioinformatics workflows with sequencer integration, automatic analysis of customizable workflows, or out-of-the-box DRAGEN pipelines.
Streamline germline variant interpretation and research report generation for rare and genetic diseases, hereditary cancer risk and other genetic screening applications, cytogenomics, and large cohort studies.
Streamline variant interpretation and reporting for somatic oncology research applications.
Perform statistical analysis and explore data with interactive, customizable, and publication-ready visualizations.
Related training
Virtual training courses: Intro to NGS
Learn how to start an NGS project and access game changing discovery power.
Learn the basics of NGS and find tips for getting started.
References
- Illumina DRAGEN secondary analysis is the first single platform to achieve 99.83% accuracy based on 2020 PrecisionFDA v2 Truth Challenge Benchmark Data. Details here.