Reagent v2.5 kits use a resilient flow cell that maintains starting fluorescent intensity, improving stability and performance compared to earlier versions.
NextSeq 500/550 v2.5 Kits deliver the power of high-throughput sequencing on the NextSeq 550 System. The v2.5 kits retain the same pre-filled, ready-to-use reagent cartridges as the v2 kits and offer improvements that provide increases in stability, robustness, and performance. The v2.5 kits offer:
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.
| Instruments | NextSeq 550 System, NextSeq 550Dx in Research Mode, NextSeq 500 System |
|---|---|
| Maximum output | 120 Gb (300-cycle high output kit), 60 Gb (150-cycle high output kit), 39 Gb (300-cycle mid output kit), 30 Gb (75-cycle high output kit), 19.5 Gb (150-cycle mid output kit) |
| Maximum reads per run | 400M clusters (high output kits), 130M clusters (mid output kits) |
| Nucleic acid type | DNA, RNA |
| Reagent type | Paired-end sequencing, Sequencing control, Cluster generation, Sequencing by synthesis, Single-read sequencing |
| Technology | Sequencing |
Required products:
Optional products:
Sequencing coverage calculator
Calculator to help determine the reagents and sequencing runs needed to arrive at desired coverage for your experiment.
Library prep and array kit selector
Find the right sequencing library preparation kit or microarray for your needs. Filter by method, species, and more. Compare, share, and order kits.
The NextSeq 550 System allows researchers to perform a broad range of applications, from mRNA-Seq to exome sequencing and more. View recommended reagent kits for popular sequencing applications. All NextSeq 500/550 kits are paired-end capable.
| NextSeq 500/550 Kits | High output Up to 400 million clusters |
Mid output Up to 130 million clusters |
|---|---|---|
300 cycles |
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150 cycles |
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75 cycles
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NextSeq 500/550 High Output Kit v2.5 (75 Cycles)
20024906
Provides kitted reagents for 75 cycles of sequencing, plus dual-indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (75 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge.
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NextSeq 500/550 High Output Kit v2.5 (150 Cycles)
20024907
Provides kitted reagents for 150 cycles of sequencing, plus dual- indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (150 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge.
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NextSeq 500/550 High Output Kit v2.5 (300 Cycles)
20024908
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (300 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge.
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NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles)
20024904
Provides kitted reagents for 150 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 130M reads). Includes: Mid Output Reagent Cartridge (150 cycles), Mid Output Flow Cell Cartridge, and Buffer Cartridge.
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NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles)
20024905
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 130M reads). Includes: Mid Output Reagent Cartridge (300 cycles), Mid Output Flow Cell Cartridge, and Buffer Cartridge.
List Price:
Discounts:
TG NextSeq™ 500/550 High Output Kit v2.5 (75 Cycles)
20024911
Provides kitted reagents for 75 cycles of sequencing, plus dual-indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (75 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge.
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Discounts:
TG NextSeq™ 500/550 High Output Kit v2.5 (150 Cycles)
20024912
Provides kitted reagents for 150 cycles of sequencing, plus dual- indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (150 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge.
List Price:
Discounts:
TG NextSeq™ 500/550 High Output Kit v2.5 (300 Cycles)
20024913
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a High Output run (up to 400M reads). Includes: High Output Reagent Cartridge (300 cycles), High Output Flow Cell Cartridge, and Buffer Cartridge.
List Price:
Discounts:
TG NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles)
20024909
Provides kitted reagents for 150 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 130M reads). Includes: Mid Output Reagent Cartridge (150 cycles), Mid Output Flow Cell Cartridge, and Buffer Cartridge.
List Price:
Discounts:
TG NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles)
20024910
Provides kitted reagents for 300 cycles of sequencing, plus dual- indexing support on a Mid Output run (up to 130M reads). Includes: Mid Output Reagent Cartridge (300 cycles), Mid Output Flow Cell Cartridge, and Buffer Cartridge.
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Users can sequence 1–12 exomes, 1–16 transcriptomes, 6–96 targeted panels, or 12–40 gene expression profiling samples per run on the NextSeq 550 System.
The NextSeq 550 flow cell is a non-patterned flow cell with distinct nanowells of varied sizes, undefined shapes, and irregular spacing for use in cluster generation. Prepared libraries are loaded onto the flow cell, which is then loaded into the NextSeq 550 System for cluster generation and sequencing.
One flow cell can be run on the NextSeq 550 System.
An update to NextSeq Control Software (NCS 2.2.0) is required to run the NextSeq v2.5 sequencing reagent kits.
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
A high-performance, fast, and reliable human whole-exome sequencing solution that includes a comprehensive, up-to-date exome enrichment panel.
Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Remove rRNA from plant leaf, seed, and root tissue.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
A fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities.
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