NOTE: An update to NextSeq Control Software (NCS 2.2.0) is required to run the NextSeq v2.5 sequencing reagent kits.
NextSeq 500/550 v2.5 sequencing reagent kits deliver the power of high-throughput sequencing on a benchtop system with increased stability and robustness. These kits offer:
The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.
Ready-to-use cartridges redefine ease-of-use
Preconfigured reagent kits for the NextSeq Series Systems provide all the components needed for sequencing on the platform in three ready-to-use cartridges (reagent, flow cell, and buffer). The kits offer simplified cartridge loading with the bleach and dual-indexing primers already in the cartridge.
Simply load user-prepared library pools directly into the NextSeq reagent cartridge. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.
This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.
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NextSeq 500/550 Kit Documentation
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Custom Protocol Selector
Generates customized, end-to-end instructions
Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Remove rRNA from plant leaf, seed, and root tissue.
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
