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See how researchers use mRNA-Seq with the NextSeq Series.
Read eBook: Gene Expression and Regulation Research with RNA-Seq
10.5 hours/4.5 hours hands-on
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
15–18 hours/15 minutes hands-on
Bring the power of a high-throughput sequencing system to the benchtop. The 150 cycle high-output or mid-output kits provide 2 x 75 bp reads for total RNA-Seq.
Push-button tools
Performs read mapping, normalized abundance estimation of reference genes and transcripts, novel transcripts assembly, variant calling, and optional fusion calling.
Quickly assess novel transcript isoforms and gene expression levels from RNA-Seq Alignment results.
Browse sample datasets on BaseSpace Sequence Hub:
NextSeq 500 RNA-Seq Run
NextSeq 500 RNA-Seq Project
See how researchers use the NextSeq Series for exome sequencing applications.
1 day/3 hours hands-on
Uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.
Nextera Flex for Enrichment uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time.
15-18 hours/15 minutes hands-on
Bring the power of a high-throughput sequencing system to the desktop. The 300 cycle high-output or mid-output kits provide 2 x 150 bp reads for exome sequencing.
Push-button tools
Rapid alignment and variant detection for small, structural, and copy number variant calling, variant annotation, and enrichment metrics calculation.
See how researchers can use the NextSeq Series to sequence whole genomes:
6 hours/4 hours hands-on
Generate WGS libraries and efficiently interrogate samples with limited available DNA.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
26–29 hours/15 minutes hands-on
Bring the power of a high-throughput sequencing system to the desktop. The 300 cycle high-output kit provides 2 x 150 bp reads for whole-genome sequencing.
Push-button tools
Quickly extracts biological information from whole-genome sequences, using Isaac alignment and variant calling.
Browse sample data sets on Basespace Sequence Hub:
NextSeq 500 WGS Run
NextSeq 500 WGS Project
In addition to sequencing, the NextSeq 550 enables microarray scanning for a broad range of applications in reproductive health, genetic health, and cancer research, including methylation analysis with the Infinium MethylationEPIC BeadChip.
Use sequence-specific hybridization to analyze genomic regions of interest.
Analyze both coding RNA and multiple forms of noncoding RNA with total RNA sequencing, for a comprehensive view of the transcriptome.
NextSeq 550 System RNA-Seq Solution
Detect both known and novel features of the RNA exome using sequence-specific capture of RNA coding regions.
Targeted resequencing focuses time, expenses, and analysis on sequencing only a subset of genes or genome regions of research interest.
Targeted gene sequencing panels contain defined probe sets focused on specific genes of interest. Both predesigned and custom panels are available.
Perform epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.
Ultra-deep sequencing of PCR amplicons enables cost-effective analysis of up to hundreds of target genomic regions in one assay.
De novo sequencing with next-generation sequencing (NGS) enables fast, accurate characterization of species without a reference genome.
NextSeq 550 System WGS Solution
Delivering Sequencing Results Quickly and at a Competitive Price
Genotyping by sequencing provides a low-cost genetic screening method to discover novel plant and animal SNPs and perform genotyping studies.
Combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP-Seq is a powerful method for genome-wide surveys of gene regulation.
Isolate and sequence small RNA species, such as microRNA, to study the role of noncoding RNA in gene silencing and posttranscriptional regulation.
Ribosome profiling enables systematic monitoring of cellular translation processes and prediction of protein abundance.
Ribosome Profiling Enables Comprehensive Translation Analysis
Comprehensively sample genes in organisms present in a complex sample to evaluate bacterial diversity and detect unculturable microorganisms.
Next-generation sequencing offers base-by-base, genome-wide detection of chromosomal variation to complement array-based cytogenomic analysis.
Find out how to utilize RNA-Seq to discover and profile RNA-based drug response biomarkers. Access resources designed to help new users adopt this application.
Quantitatively interrogate methylation sites across the genome at single-nucleotide resolution.
Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.
Access Guide