NextSeq 1000 & NextSeq 2000 Systems

 
 
 
 

Innovative sequencing for more biological discoveries

The NextSeq 1000 and NextSeq 2000 Systems are enabling biological insights with breakthrough innovative features thoughtfully designed in a compact, yet versatile platform. With up to 14 configurations and read lengths from 1x50bp to 2x300bp, these systems efficiently handle benchtop workflows with flexibility and scalability. Efficient design combined with intuitive workflows deliver ease of use while driving down costs and minimizing waste. Confidently expand your research with single-cell and spatial sequencing and other emerging applications with the NextSeq 1000 and NextSeq 2000 Systems.

NextSeq P4 flow cell

Robust performance powered by an intuitive workflow

These systems offer operational ease and onboard informatics, simplifying workflows for both new and advanced users. Advanced XLEAP-SBS chemistry, built on the proven foundation of standard Illumina SBS chemistry, enables faster and higher quality sequencing than ever before. Coupled with high-efficiency and low-cost workflows, the NextSeq 1000 and NextSeq 2000 Sequencing Systems deliver technological advancements for diverse sequencing applications.

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Delivering sequencing success through community

The NextSeq 1000 and NextSeq 2000 Systems offer the certainty of a field-tested solution and a reliable sequencing partner. Joining this community gives access to a large ecosystem of applications, protocols, and informatics that have been built in collaboration with thousands of researchers and industry thought leaders across the globe.

 
 
 
 

Sequencing flexibility for the applications you need

The NextSeq 1000 and NextSeq 2000 are redesigned from the ground up. Their flexibility, affordability, and scalability help both new and experienced users achieve fast turnaround times and reduced operating costs. With the wide range of available flow cells, they support a variety of applications including:

Single-Cell Gene Expression

Examine the transcriptomes of individual cells for a high-resolution view of cell-to-cell variation.

Whole-Exome Sequencing

Efficiently identify coding variants across a broad range of applications, including population genetics, genetic disease, and cancer studies.

Shotgun Metagenomics

Comprehensively sample all genes in all organisms present in a given complex sample.

Spacial Transcriptomics

Pinpoint predictive cellular state and their spatial localization to understand the influence of the cellular and spatial architecture on the microenvironment.

Total RNA Sequencing

Analyze both coding RNA and multiple forms of noncoding RNA for a comprehensive view of the transcriptome.

Infectious Disease Research

Infectious Disease Research Description: A universal, culture-free method for infectious disease characterization and surveillance that may be used with viruses, bacteria, fungi, and parasites, and can replace the need for multiple tests.

Expand Your Access to More Data and Applications

High Intensity Sequencing

High-intensity sequencing applications generate dramatically more data for more impactful insights.

Multiomics

Perform transformative science with access to multiple emerging high-throughput omics applications.

Efficient and Cost-Effective

Researchers talk about their experience using the NextSeq 1000 and NextSeq 2000 Sequencing Systems.

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Efficient and Cost-Effective

System Specifications

10-540 Gb*
output range
100M -1.8B
reads per run
2 x 300 bp
max read length

*Specifications based on Illumina PhiX control library at supported cluster densities.

See why researchers choose Illumina benchtop sequencers for their lab

When you partner with Illumina, you’ll get more than just leading-edge technology–you become part of a community with like-minded goals.

Learn more

Dr. Hoa Giang of Gene Solutions
The NextSeq 1000 runs P1 and P2 flow cells, offering fast turnaround times, lower throughput, and a lower cost instrument. The NextSeq 2000 accesses a wider range of throughputs with P1, P2, and P3 flow cells for more sequencing per sample, larger studies, and better run economics.
Both systems use patterned flow cells to offer the highest cluster density flow cell of any on-market NGS system to date, driving down the run cost. In these flow cells, monitoring the Cluster PF (%) is a better measure of potential cluster occupancy than measuring cluster density..
Upgrading from a NextSeq 1000 instrument to a NextSeq 2000 instrument is seamless. Contact us for more information.

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