SureCell WTA 3′ Library Prep Kit for the ddSEQ System

A robust, scalable, and user-friendly workflow for transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.Read More...
Select Product(s)

SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)

20014279

Price
 
 

SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (6 Cartridges Kit)

20014280

Price
 
 

Product Highlights

The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing studies.

  • Sensitive and unbiased characterization of transcriptional signatures – Highly sensitive and reproducible gene detection in varied individual cells
  • Comprehensive single-cell RNA sequencing workflow – Fully supported workflow developed in collaboration by technology innovators
  • Powerful next-generation sequencing integrated with simple data analysis – Proven Illumina sequencing combined with streamlined, user-friendly analysis software

Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.

Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.

Data Analysis

Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.

View sample data (requires a BaseSpace login)

Species Details

This kit has been validated for human and mouse samples. The BaseSpace Sequence Hub SureCell RNA Single-Cell App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 4 to 6 samples per run (based on a minimum of 100,000 reads per cell) read 1: 68 bp, read 2: 75 bp
HiSeq 2500 System Rapid run: 4-5 samples per lane; high output v4: 4 samples per lane (based on a minimum of 100,000 reads per cell) read 1: 68 bp, read 2: 75 bp

Product Comparison

SureCell WTA 3′ Library Prep Kit for the ddSEQ System TruSeq RNA Library Prep Kit v2 TruSeq Stranded mRNA TruSeq RNA Exome
Assay Time 11-12 hours ~10.5 hours ~10.5 hours ~2 Days
Content Specifications Captures the coding transcriptome (3' enriched), with strand-specific information Captures the coding transcriptome (without strand information) Captures the coding transcriptome with strand information Captures the coding transcriptome/RNA exome
Description Enables single-cell RNA-Seq studies using a droplet generator that isolates ~1200 cells per cartridge, and a simple library prep process. A simple, cost-effective research solution for analysis of the coding transcriptome. Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information. Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information and a reduced sample input requirement.
Hands-On Time 4-5 hours ~4.5 hours ~4.5 hours ~11 hours
Input Quantity ~45,000 cells per cartridge (required input) 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails) 0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails) 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples
Mechanism of Action Oligo dT capture, single cell Oligo-dT beads capture polyA tails Oligo-dT beads capture polyA tails Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails.
Method mRNA Sequencing mRNA Sequencing mRNA Sequencing Exome Sequencing , mRNA Sequencing
Multiplexing 1-24 Up to 24-plex per lane 1–96 Up to 24 single, 96 combinatorial (CD) dual
Specialized Sample Types Not FFPE-Compatible, Single Cells Not FFPE-Compatible Not FFPE-Compatible FFPE Tissue, Low-Input Samples
Species Category Human, Mouse Bovine, Human, Mammalian, Mouse, Rat Bovine, Human, Mammalian, Mouse, Rat Human
Strand Specificity Stranded Non-Stranded Stranded Stranded

Method-Specific Workflow Example

 

Supporting Data and Figures

 

Related Products