With targeted resequencing, a subset of genes or regions of the genome are isolated and sequenced. Target enrichment works by capturing genomic regions of interest by hybridization to target-specific biotinylated probes, which are then isolated by magnetic pulldown.
Nextera Flex for Enrichment is the latest innovation in next-generation sequencing (NGS) target enrichment solutions. By combining bead-linked transposome-mediated tagmentation chemistry with hybrid-capture target enrichment, researchers can reduce workflow time and produce an accurate, reliable assay for target enrichment resequencing.
Hybridization-based enrichment is a useful strategy for analyzing specific genetic variants in a given sample.
Target enrichment allows researchers the ability to reliably sequence exomes or large numbers of genes (e.g. > 50 genes) using robust and straightforward workflows. It delivers dependable results across a wide range of input types and quantities. Uses include:
Hybrid capture-based target enrichment lets researchers target regions of the genome that are relevant to their specific research interests. Targeted resequencing delivers higher discovery power (ability to identify novel variants), and higher mutation resolution than PCR or Sanger sequencing.
Compared to PCR-based amplicon sequencing, hybridization-based enrichment sequencing can target a higher amount of total gene content and support more comprehensive profiling of all variant types. The larger amount of total gene content allows for the characterization of both known and novel variants for discovery-related applications.
|Larger gene content, typically > 50 genes||Smaller gene content, typically < 50 genes|
|More comprehensive profiling for all variant types||Ideal for analyzing single nucleotide variants and insertions/deletions (indels)|
|More comprehensive method, but with longer hands-on time and turnaround time**||More affordable, easier workflow|
* Data on file, Illumina 2018.
** The turnaround time is for library prep assay time (DNA to finished library).
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