Respiratory Pathogen ID/AMR Enrichment Kit (Powered by IDbyDNA Explify)

This NGS-based workflow targets respiratory pathogens and antimicrobial resistance alleles, and offers simplified data analysis powered by IDbyDNA. Read More...
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Respiratory Pathogen ID/AMR Enrichment Kit Set A (RUO) (96 indexes, 96 samples)

20047050

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Respiratory Pathogen ID/AMR Enrichment Kit Set B (RUO) (96 indexes, 96 samples)

20046969

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Product Highlights

The Respiratory Pathogen ID/AMR Enrichment Kit (RPIP) is a next-generation sequencing based, targeted enrichment workflow that delivers highly sensitive, comprehensive pathogen detection and antimicrobial resistance (AMR) insights for 1200+ AMR alleles, 180+ bacteria, 50+ fungi, and 40+ viruses, including SARS-CoV-2. Powered by the IDbyDNA Explify Analysis Software, the flexible and scalable end-to-end workflow, coupled with broad identification of respiratory pathogens and antimicrobial resistance markers in <24 hours, delivers a rapid and cost-effective solution for detection of respiratory tract infections in clinical research settings.

Features of this workflow:

  • Universal Detection of Respiratory Pathogens — Simultaneous detection of RNA and DNA for 180+ bacteria, 50+ fungi, and 40+ viruses, including SARS-CoV-2. The RPIP target enrichment workflow delivers comprehensive detection of critical respiratory pathogens.
  • Detection of Antimicrobial Resistance Genes — Detects 1200+ AMR alleles and provides insights for 16 classes of antibiotics.
  • Strain Typing of Critical Pathogens – Delivers whole-genome information for SARS-CoV-2 and Flu A/B viruses to support public health surveillance and epidemiology.
  • Quality Control — Includes probes targeting 10 commercially available spike-in control options.
  • Cost-Effective — Comprehensive panel design enables cost-effective detection of RNA and DNA respiratory pathogens and antimicrobial resistance alleles all at the same time, from the same assay.
  • Rapid – Delivers simultaneous detection of bacteria, viruses, fungi, and AMR alleles in <24 hours using the MiniSeq Rapid Reagent Kit.

Powerful, Simple Data Analysis with the IDbyDNA Explify Platform

The Explify Respiratory ID/AMR Analysis App offers a paired and tailored data analysis solution for the Illumina Respiratory Pathogen ID/AMR Enrichment Kit. The app provides information on sample composition, including host and microbial abundance, and proportion of targeted vs. untargeted sequences.

This app is available in BaseSpace Sequence Hub. Users can select up to 35 individual samples or project folders containing any number of samples for analysis. Reports include a detailed text-based (JSON) and PDF file format.

Access this app in BaseSpace Sequence Hub

See the Explify Respiratory ID/AMR Analysis User Guide for more info.

Frequently Purchased Together

Specifications

Product Comparison

Respiratory Pathogen ID/AMR Enrichment Kit (Powered by IDbyDNA Explify) Respiratory Virus Oligo Panel
Assay Time < 9 hours library prep time < 9 hours library prep time
Automation Capability Liquid Handling Robots Liquid Handling Robots
Description A streamlined workflow using Respiratory Pathogen ID/AMR Panel (RPIP) with Illumina RNA Prep with Enrichment, sequencing on Illumina desktop systems, and powerful analysis using IDbyDNA’s Explify Platform allows researchers to identify respiratory infections and co-infections, identify antimicrobial resistance, and perform strain typing of critical pathogens (SARS-CoV-2 and Flu A/B viruses) to study viral evolution and transmission. A streamlined workflow for detecting and analyzing SARS-CoV-2 and other common respiratory viruses using RNA Prep for Enrichment combined with Respiratory Virus Oligo Panel for library prep, proven Illumina sequencing, and simplified data analysis using the DRAGEN RNA Pathogen Detection App or IDbyDNA Explify RVOP app on BaseSpace Sequencing Hub.
Hands-on time < 2 hours library prep time < 2 hours library prep time
Multiplexing Up to 384 samples in a single run with unique dual indexes Up to 384 samples in a single run with unique dual indexes
Species Category Bacteria, Fungal, Virus Human, Virus
Species Details Detects respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses, including SARS-CoV-2) and antimicrobial resistance alleles (1200+). Detects common respiratory viruses, including recent flu strains and SARS-CoV-2. Includes human probes as a quality feature, human probes are included in every sample.
System Compatibility MiniSeq , MiSeq , MiSeq, NextSeq 550 MiniSeq , MiSeq , MiSeq, NextSeq 550

Example Workflow

 

Supporting Data and Figures

 

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