Achieve exceptionally rapid targeted RNA enrichment with the new Illumina RNA Prep with Enrichment. Use code RNA25 at checkout to receive 25% off your first order, now through the end of 2020.* 

*Offer valid for library prep kit only. Index adapters and panels are not included in this promotion.

TruSeq RNA Exome

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.Read More...
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Library Prep

TruSeq® RNA Library Prep for Enrichment (48 Samples)

20020189

Price
 
 

Enrichment

TruSeq® RNA Enrichment (12 enrichments)

20020490

Price
 
 

Index Adapters

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

Price
 
 

TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

Price
 
 

Panel

Illumina Exome Panel – Enrichment Oligos Only

20020183

Price
 
 
Accessory Products

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Price
 
 

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

Price
 
 

Product Highlights

TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.

  • Affordability and focus—Isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth
  • High-quality data from difficult samples—Optimized for sequencing RNA from degraded samples, including FFPE tissues
  • Samples with limited starting material—Greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity

TruSeq RNA Exome generates RNA sequencing (RNA-Seq) libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions to help maximize discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis. Reagent volumes supplied are sufficient to support 1- to 4-plex enrichment reactions.

Find automation vendors with robotic systems compatible with this product

Read a comprehensive assessment of Illumina’s ribosomal RNA depletion protocol

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 5 to 16 samples per run (based on 25 million reads per sample) 2 x 75 bp
HiSeq 2500 System 24-160 samples per run (dual flow cell; based on 25 million reads per sample) 2 x 75 bp
NovaSeq 6000 System Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (currently limited by available index combinations) 2 × 100 bp

Product Comparison

TruSeq RNA Exome Illumina RNA Prep with Enrichment Illumina Stranded mRNA Prep
Assay Time ~2 Days < 9 hours 6.5 hours
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Content Specifications Captures the coding transcriptome/RNA exome Captures the coding transcriptome when used with Illumina Exome Panel Captures the coding transcriptome with strand information
Description Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information and a reduced sample input requirement. A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information
Hands-On Time ~11 hours < 2 hours < 3 hours
Input Quantity 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples 10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples 25-1000 ng standard-quality total RNA
Mechanism of Action Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails. Bead-linked transposome PolyA capture, ligation-based addition of adapters and indexes
Method Exome Sequencing , mRNA Sequencing mRNA Sequencing , Target Enrichment , Target Enrichment, Targeted RNA Sequencing mRNA Sequencing
Multiplexing Up to 24 single, 96 combinatorial (CD) dual Up to 384 Unique Dual Indexes (UDIs) Up to 384 Unique Dual Indexes (UDIs)
Specialized Sample Types FFPE Tissue, Low-Input Samples Blood, FFPE Tissue, Low-Input Samples Low-Input Samples, Not FFPE-Compatible
Species Category Human Human, Virus Bovine, Human, Mammalian, Mouse, Rat
Strand Specificity Stranded Non-Stranded Stranded

The product previously known as the TruSeq RNA Access Library Prep Kit (Cat. No. RS-301-2001 and RS-301-2002) is now called TruSeq RNA Exome. The product configuration has changed. In the new configuration, major components such as library preparation, index adapters, enrichment reagents, and exome panels can be purchased separately. If you were previously buying Cat. No. RS-301-2001 or Cat. No. RS-301-2002, purchase the following components: TruSeq RNA Library Prep for Enrichment (Cat. No. 20020189), TruSeq RNA Enrichment (Cat. No. 20020490), Exome Panel (Cat. No. 20020183), and one item from the Index Adapters section below.

Method-Specific Workflow Example

 

Customer Stories

Looking Beyond Single-Drug Approaches to Precision Oncology Research

Researchers rely on transcriptomics to uncover multiple drug-susceptible tumorigenic pathways.

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A Tale of Two RNA Library Prep Kits

A critical comparison between two popular TruSeq RNA Library Prep Kits reveals new information of interest to researchers conducting RNA sequencing studies.

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Supporting Data and Figures

Efficient Gene Fusion Discovery
Supporting Data and Figures

TruSeq RNA Exome enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.

 

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