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Library Prep Automation

Automation Methods that Span Our Library Prep Portfolio

Minimize errors, reduce hands-on time, and enable higher throughput

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Automate Your Library Prep

Automating library preparation can help increase your lab's efficiency and generate more consistent results. Automation can also help you maintain turnaround times, despite expanding sample workloads. To aid with adopting NGS automation, methods for many of our library prep assays are available from the leading automation companies. Our partners offer a range of automation platforms to address your laboratory's throughput needs.

Both Illumina and our partners have created methods to address the increasing range of automation products and library prep. Methods created by a partner are tested, distributed, and typically supported by that partner. Illumina-developed methods are distributed by Illumina and are usually supported by our partners.

Illumina Qualified Methods are available for several of our library prep kits. We verify that libraries created with these methods perform comparably to manually prepared libraries. These methods are developed by partners who test for functional performance and provide distribution and support.

We make it easy to automate your library prep. The vendors below have methods designed for Illumina kits, eliminating the time and effort needed to develop your own method.

  Agilent Beckman Coulter Eppendorf Hamilton PerkinElmer Tecan
Illumina Stranded Total RNA     epMotion 5075t STAR NGS    
Illumina Stranded mRNA     epMotion 5075t STAR NGS    
Illumina RNA Prep with Enrichment   Biomek i7 epMotion 5075t   Sciclone  
Illumina DNA PCR Free   Biomek i5
Biomek i7
epMotion 5075t NGS STAR Sciclone
Janus
Fluent
Illumina DNA Prep Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone Freedom EVO NGS
Illumina DNA Prep with Enrichment Bravo Option B Biomek iSeries epMotion 5075t NGS STAR Scicione Freedom EVO NGS
Nextera XT DNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Scicione Freedom EVO NGS
AmpliSeq for Illumina*   Biomek i5 epMotion 5075t
epMotion 5073
NGS STAR
NGS STARlet
Scicione  
TruSight Oncology 500   Biomek NXp
Biomek i5
  NGS STAR    
TruSight Tumor 15   Biomek 4000 epMotion 5075t   Zephyr  
TruSight Tumor 170   Biomek NXp   NGS STAR    
TruSeq DNA PCR-Free Bravo Option B Biomek FXp epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
TruSeq Nano DNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
TruSeq RNA Exome Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
TruSeq RNA Fusion     epMotion 5075t      
TruSeq RNA Pan Cancer            
TruSeq RNA v2   Biomek FXp     Scicione NGS and NGSx  
TruSeq Stranded mRNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
TruSeq Stranded Total RNA Bravo Option B Biomek FXp epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
16S rRNA     epMotion 5075t
epMotion 5073
NGS STAR    

Illumina Qualified (Illumina analysis has shown that libraries prepared with these methods perform comparably to those prepared manually)

Documentation: user guide and / or app note available

*In order to accommodate for higher dead volume requirements associated with automated platforms and variation in overfill volumes by the original reagent manufacturer, more than one AmpliSeq for Illumina kit may be required when preparing the maximum number of libraries per kit.

Frequently Purchased Together

Automated Library Prep Workflow Examples

Assessing Nucleic Acid Quality with Fragment Analyzer™

The Fragment Analyzer offers automated fragmentation and size selection. It accurately assesses quality and quantity of prepared libraries to optimize cluster generation during sequencing, and it accommodates both low- and high-throughput labs.

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Automated Nextera DNA Flex Library Prep (now Illumina DNA Prep) Workflow for High-Throughput Metagenomics

The streamlined, automated workflow generates highly uniform libraries and provides excellent data for species identification, metagenomic profiling, and de novo genome assembly.

Download

Library Prep Technical Tips

DNA/RNA Isolation Considerations

Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.

dsDNA Library Concentration

Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.

Quantification and Quality Control

Find out how to quantify and validate final libraries for a successful sequencing run.