iSeq 100 Reagents

Reagents for the iSeq 100 System feature a ready-to-use cartridge with one-channel SBS chemistry and CMOS technology for simple, low-cost sequencing.

Overview

iSeq 100 Reagents leverage complementary metal-oxide semiconductor (CMOS) technology to deliver one-channel sequencing by synthesis chemistry, significantly reducing the cost of sequencing compared to two- and four-channel chemistries. This allows labs of all sizes to take advantage of the power and reliability of Illumina next-generation sequencing (NGS) technology at an affordable price.

Key features

  • Support for both single-read and paired-end sequencing

  • Versatility in applications ranging from cancer panels to small-genome sequencing

  • A simple workflow that integrates all reagents into a single cartridge

  • Optimized chemistry to reduce the occurrence of no-cluster run failures

  • Longer storage time, providing greater flexibility for reagent staging and run planning

  • The generation of a high percentage of bases above Q30 on the iSeq 100 System

Learn more about CMOS technology

Specifications

Instruments iSeq 100 System
Maximum output 1.2 Gb
Maximum reads per run Up to 8 million (paired-end reads); up to 4 million (single reads)
Nucleic acid type DNA, RNA
Reagent type Paired-End Sequencing, Sequencing Control, Cluster Generation, Sequencing by Synthesis, Single-Read Sequencing
Technology Sequencing

iSeq 100 Reagents Performance Parameters

Read Length Reads Per Run (Passing Filter) Output Bases > Q30 Run Time
1 × 36 bp 4 M 144 Mb > 85% 9 hrs
1 × 50 bp 4 M 200 Mb > 85% 9 hrs
1 × 75 bp 4 M 300 Mb > 80% 10 hrs
2 × 75 bp 4 M 800 Mb > 80% 13 hrs
2 × 150 bp 4 M 1.2 Gb > 80% 19 hrs
 
4 M
4 M

Documentation

Product literature
Support documentation

A dedicated support section is not currently available for this product

Compare

Selection Tools:

Sequencing Support – Coverage Calculator

Calculator to help determine the reagents and sequencing runs needed to arrive at desired coverage for your experiment.

Resources

Thumbnail

iSeq 100: Introduction

iSeq 100 i1 Reagent v2 (300-cycle)

20031371

Includes one i1 flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 300-cycle run on the iSeq 100 System.

Sign in to add to cart or see pricing.

List Price:

Discounts:

iSeq 100 i1 Reagent v2 (300-cycle) 4 pack

20031374

Includes four i1 flow cells and four pre-filled cartridges containing clustering and sequencing reagents to support 4 × 300-cycle runs on the iSeq 100 System.

Sign in to add to cart or see pricing.

List Price:

Discounts:

iSeq 100 i1 Reagent v2 (300-cycle) 8 pack

20040760

Includes eight i1 flow cells and eight pre-filled cartridges containing clustering and sequencing reagents to support 8 × 300-cycle runs on the iSeq 100 System.

Sign in to add to cart or see pricing.

List Price:

Discounts:

Showing of

Selection summary

Product

Qty

Unit Price

Total ( items)

You have not selected any items yet.

Please add these products to your cart before leaving the page to avoid losing them.

FAQs

Yes, iSeq 100 i1 Reagents include all consumables to support both single-read and paired-end sequencing. During run setup, the iSeq 100 System can be configured to generate either single-read or paired-end data.

The 300-cycle reagents support up to 322 cycles of sequencing to allow for dual 10 bp indexes on a 2 × 151 run.

No, DNA denaturation is performed on the cartridge.

A patterned flow cell has billions of ordered nanowells that are manufactured into the flow cell glass. The ordered wells allow for the generation of sequencing clusters in an ordered arrangement. Clusters are aligned more closely together, increasing the number of output reads and amount of sequence data generated. The iSeq 100 Reagents cartridge contains a patterned flow cell fabricated over a CMOS chip. Learn more about patterned flow cells

v1 reagents have been discontinued and replaced with iSeq 100 i1 Reagents v2. 
 
Note: iSeq 100 Software System Suite v2.0 or later is required to run the iSeq 100 i1 Reagents v2 kits. 

of

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

COVIDSeq Assay (96 samples)

This low- to mid-throughput NGS assay enables labs of any size to identify and track the emergence and prevalence of novel SARS-CoV-2 variants.

Illumina Microbial Amplicon Prep

A flexible and streamlined NGS library prep kit that enables various public health surveillance and microbial research applications.

Nextera DNA Indexes

Nextera DNA Indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput.

TruSight One Sequencing Panels

Comprehensive sequencing research panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.

Speak with a specialist

Reach out for information about our products and services, or get answers to questions about our technology.