TruSight Cancer

These expert-defined sequencing research panels target 94 genes and 284 SNPs associated with a predisposition towards various cancers.

1.5 days

Assay time

5 hr

Hands-on time

50 ng DNA

Input quantity

See full details in the specifications table

Overview

Developed in collaboration with cancer genomics experts, these predesigned, ready-to-use oligos enable researchers to sequence a variety of genes and single nucleotide polymorphisms (SNPs) previously linked to cancer predisposition.

  • Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatible with TruSight Rapid Capture.

TruSight Cancer sample datasets

6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

This product is available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.


Specifications


Required products

Required Accessories

You’ll also need one or more of the following enrichment products.

/ Results

Applications

Example workflow


Project recommendations

Instrument Recommended number of samples Read length
MiniSeq System

Samples per run: mid output: 12, high output: 24 (based on > 20x coverage per target)

Up to 2 × 150 bp

MiSeq System

Samples per run (by reagent kit version): v2: 12, v3: 24 (based on > 20x coverage per target)

Up to 2 × 150 bp

NextSeq 550 System

Samples per run: mid output: 96, high output: 96 (based on > 20x coverage per target)

Up to 2 × 150 bp


Related applications and methods

How others use this product

Researchers from the MGZ Medical Genetic Center are using the Infinium CytoSNP-850K BeadChip and the TruSight Cancer Panel to analyze chromosomal abnormalities.

Documentation

Product literature

Compare

TruSight Cancer TruSight Hereditary Cancer Panel AmpliSeq for Illumina BRCA Panel
Assay time 1.5 days

~48 hr from DNA to data

5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer type Pan-Cancer Pan-Cancer, Solid Tumor Solid Tumor
Content specifications Fixed set of probes that enrich for 94 genes and 284 SNPs associated with common and rare cancers. Targets 113 cancer risk-associated genes Exonic regions and the flanking intronic sequences of the BRCA1 and BRCA2
Description Germline mutation detection research for common and rare cancers.

The TruSight Hereditary Cancer Panel is a targeted sequencing panel designed to assess germline mutations across 113 genes and 125 single nucleotide polymorphisms (SNPs) for identification purposes and polygenic risk scoring. 

Germline and somatic analysis studies of BRCA1 and BRCA2.
Hands-on time 5 hr ~2 hr <1.5 hr
Input quantity 50 ng DNA

50–1000 ng DNA 

1–100 ng (10 ng recommended per pool)
Instruments MiSeq System, NextSeq 550 System, MiSeqDx in Research Mode, MiniSeq System, NextSeq 500 System MiSeq System, iSeq 100 System, NextSeq 550 System, MiniSeq System, NextSeq 550Dx in Research Mode, NextSeq 500 System MiSeq System, iSeq 100 System, MiSeqDx in Research Mode, MiniSeq System
Method Targeted DNA sequencing, Target enrichment Targeted DNA sequencing, Target enrichment Targeted DNA sequencing, Amplicon sequencing
Multiplexing Up to 96-plex 96 dual index combinations
Nucleic acid type DNA DNA DNA
Specialized sample types Not FFPE-Compatible, Not FFPE-Compatible Blood, Saliva Blood, FFPE Tissue
Species category Human Human Human
Technology Sequencing Sequencing Sequencing
Variant class Germline variants Germline variants, Insertions-deletions (indels), Copy number variants (CNVs) Single nucleotide polymorphisms (SNPs), Somatic variants, Germline variants, Insertions-deletions (indels)

Enrichment panels (12)

TruSight Cancer – Enrichment Oligos only (4 or 8 Enrichment Reactions)

FC-121-0202

TruSight Cancer targets 94 genes and 284 SNPs associated with a predisposition towards various cancers and is sufficient for 8 enrichment reactions when paired with Nextera Flex for Enrichment due to a single hybridization workflow and sufficient for 4 enrichment reactions when paired with TruSight Rapid Capture. Library prep, enrichment, and index adapter reagents need to be ordered separately.

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Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)

20025524

Includes reagents for preparing and enriching 96 libraries (eight, 12-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.

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Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)

20025523

Includes reagents for preparing and enriching 16 libraries (16, 1-plex enrichment reactions). Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately.

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Illumina® DNA Prep, (S) Tagmentation (96 Samples)

20025520

Includes reagents for preparing 96 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (96 samples)).

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Illumina® DNA Prep, (S) Tagmentation (16 Samples)

20025519

Includes reagents for preparing 16 libraries. No enrichment reagents are included. Purchase enrichment probe panel, Illumina Purification Beads and index adapters separately. Enrichment reagents are only orderable with library prep reagents (Illumina DNA Prep with Enrichment, (S) Tagmentation (16 samples)).

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IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

Includes 96, 10 bp indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.

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TruSight Rapid Capture (1 index, 8 samples, 8 enrichments)

FC-140-1101

Contains reagents to prepare up to 8 libraries through eight 1-plex enrichments. Includes 1 unique index combination.

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TruSight Rapid Capture (2 indexes, 8 samples, 4 enrichments)

FC-140-1102

Contains reagents to prepare up to 8 libraries through four 2-plex enrichments. Includes 2 unique index combinations.

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TruSight Rapid Capture (4 indexes, 16 samples, 4 enrichments)

FC-140-1103

Contains reagents to prepare up to 16 libraries through four 4-plex enrichments. Includes 4 unique index combinations.

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TruSight Rapid Capture (24 indexes, 48 samples, 4 enrichments)

FC-140-1104

Contains reagents to prepare up to 48 libraries through four 12-plex enrichments. Includes 24 unique index combinations.

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TruSight Rapid Capture (24 indexes, 96 samples, 8 enrichments)

FC-140-1105

Contains reagents to prepare up to 96 libraries through eight 12-plex enrichments. Includes 24 unique index combinations.

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TruSight Rapid Capture (96 indexes, 288 samples, 24 enrichments)

FC-140-1106

Contains reagents to prepare up to 288 libraries through twenty-four 12-plex enrichments. Includes 96 unique index combinations.

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Illumina Advantage Products (1)

TG TruSight™ Cancer Sequencing Panel

TG-141-1002

Contains oligos for Cancer genes focused on genes linked to predisposition to cancer and associated conditions. Sufficient volume for 4 enrichment reactions.

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Selection summary

Product

Qty

Unit Price

Order the NovaSeq X Series

Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.

MiSeq Reagent Kit v3

Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.

TruSight Oncology 500

Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Note regarding biomarker patents and other patents unique to specific uses of products:
Some genomic variants, including some nucleic acid sequences, and their use in specific applications may be protected by patents. Customers are advised to determine whether they are required to obtain licenses from the party that owns or controls such patents in order to use the product in customer's specific application.

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