Reagent kits for the NovaSeq 6000 System consisting of ready-to-use pre-filled reagent cartridges and flow cells.
NovaSeq 6000 Reagent Kits enable tunable output and proven performance for a broad range of applications on the NovaSeq 6000 system. These kits offer:
Improvements to the NovaSeq 6000 Reagent Kits include an enhanced reagent chemistry to streamline the workflow and accommodate more complex indexing strategies, an extended reagent shelf life of six months to provide project planning flexibility, and radio-frequency identification (RFID) to ensure reagent traceability and compatibility.
SP and S1 provide the fastest run times and are ideal for smaller batch sizes or in situations where rapid turnaround time is required.
S2 offers a quick, powerful, and cost-effective option for high-throughput applications.
S4 delivers high throughput while enabling cost-effective sequencing across a range of applications and depth of coverage.
NovaSeq 6000 Reagent Kits v1.5 also provide an improved Q30 quality score compared to the v1.0 reagents, support for unique molecular identifiers (UMIs), and support for whole-genome sequencing with Illumina DNA PCR-Free Library Prep.
| Instruments | NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System |
|---|---|
| Maximum output | 6000 Gb (for a dual S4 flow cell run on the NovaSeq 6000 system) |
| Maximum reads per run | 20 billion (for a dual S4 flow cell run on the NovaSeq 6000 system) |
| Nucleic acid type | DNA, RNA |
| Reagent type | Paired-end sequencing, Cluster generation, Sequencing by synthesis, Single-read sequencing |
| Technology | Sequencing |
No accessories are required, but additional library tubes for the NovaSeq 6000 Reagent Kits are available to order separately.
Sequencing Coverage Calculator
Calculator to help determine the reagents and sequencing runs needed to arrive at desired coverage for your experiment.
NovaSeq 6000 S4 Reagent Kit v1.5 (300 cycles)
20028312
Includes one S4 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 300-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S4 Reagent Kit v1.5 (200 cycles)
20028313
Includes one S4 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S4 Reagent Kit v1.5 (35 cycles)
20044417
Includes one S4 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 35-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S2 Reagent Kit v1.5 (300 cycles)
20028314
Includes one S2 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 300-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles)
20028315
Includes one S2 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles)
20028316
Includes one S2 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 100-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S1 Reagent Kit v1.5 (300 cycles)
20028317
Includes one S1 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 300-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles)
20028318
Includes one S1 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 S1 Reagent Kit v1.5 (100 cycles)
20028319
Includes one S1 flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 100-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 SP Reagent Kit v1.5 (500 cycles)
20028402
Includes one SP flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 500-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 SP Reagent Kit v1.5 (300 cycles)
20028400
Includes one SP flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 300-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 SP Reagent Kit v1.5 (200 cycles)
20040719
Includes one SP flow cell on,e buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 200-cycle run on the NovaSeq 6000 System.
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NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles)
20028401
Includes one SP flow cell, one buffer cartridge, one cluster cartridge, and one sequencing cartridge to support a 100-cycle run on the NovaSeq 6000 System.
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NovaSeq Library Tubes Accessory Pack (24 tubes)
20014015
Includes 24 NovaSeq 6000 Library Tubes.
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NovaSeq 6000 Reagent Kits support both single-read and paired-end sequencing. During run setup, a run can be configured to generate single-read or paired-end data.
Yes. For NovaSeq reagents v1.5, follow the NovaSeq Series Custom Primers Guide.
The NovaSeq 6000 S4 flow cell provides 8–10B single reads and 16–20B paired-end reads.
The NovaSeq SP flow cell provides 650–800M single reads and 1.3–1.6B paired-end reads.
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI) from FFPE tissue, with added scalability.
Streamlined RNA-Seq solution for clear and comprehensive analysis across the coding and non-coding transcriptome with exceptional study flexibility.
A high-performance, fast, and reliable human whole-exome sequencing solution that includes a comprehensive, up-to-date exome enrichment panel.
NovaSeq 6000 Xp Workflow delivers the flexibility of independent lane loading and high library multiplexing per flow cell, maximizing sample throughput on a single run.
Unique dual (UD) indexes enable preparation of high-quality libraries that can be multiplexed for increased throughput and cost-saving benefits.
An efficient, fast, and integrated library preparation workflow that yields highly accurate data for sensitive sequencing applications.
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