Multiplex sequencing allows large numbers of libraries to be pooled and sequenced simultaneously during a single run on a high-throughput instrument. Sample multiplexing is useful for many applications, from targeted panels to whole human genome sequencing.
Individual "barcode" sequences are added to each DNA fragment during next-generation sequencing (NGS) library preparation so that each read can be identified and sorted before the final data analysis. Pooling samples exponentially increases the number of samples analyzed in a single run, without drastically increasing cost or time.
Researchers leverage the speed and multiplexing capabilities of Illumina sequencers to identify microbes along the Mississippi River.
Two index options are available for multiplex sequencing: combinatorial dual (CD) indexes and unique dual (UD) indexes. In contrast to CD indexing, UD indexing has completely unique indexes.
Learn how this approach can help ensure that your libraries will be sequenced and demultiplexed with the highest accuracy on Illumina sequencing platforms.
Read BulletinThis guide provides recommendations for preparing libraries with balanced index combinations for multiplexing and sequencing on Illumina systems.
Access GuideThis tool helps you calculate volumes and concentrations when pooling multiple libraries.
Access ToolResearchers at the Chan Zuckerberg Biohub are working to conduct innovative experiments and form new collaborations in genomics. The NovaSeq System enables them to bundle many samples together for medical research that may help to improve human health.
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The NovaSeq 6000 System offers scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.