TruSight Oncology 500 High-Throughput

Enabling comprehensive genomic profiling from FFPE samples, with added flexibility to batch up to 192 samples per flow cell. Read More...

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Manual Prep

TruSight Oncology 500 DNA High-Throughput (48 Samples)

20040765

Price
 
 

TruSight Oncology 500 DNA High-Throughput Kit (144 Samples)

20040767

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput (24 Samples)

20040764

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples)

20040766

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput Kit (24 Samples), plus Pierian

20040768

Price
 
 

TruSight Oncology 500 DNA High-Throughput Kit (48 Samples), plus Pierian

20040769

Price
 
 

TruSight Oncology 500 DNA/RNA High-Throughput Kit (72 Samples), plus Pierian

20040770

Price
 
 

TruSight Oncology 500 DNA High-Throughput Kit (144 Samples), plus Pierian

20040771

Price
 
 

Automation

TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples)

20049283

Price
 
 

TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples)

20049282

Price
 
 

TruSight Oncology 500 High-Throughput DNA for Automation (64 Samples), Plus Velsera

20049277

Price
 
 

TruSight Oncology 500 High-Throughput DNA/RNA for Automation (32 Samples), Plus Velsera

20049276

Price
 
 

TruSight Oncology 500 High-Throughput DNA for Automation (144 Samples)

20049285

Price
 
 

TruSight Oncology 500 High-Throughput DNA for Automation (144 Samples), Plus Velsera 

20049279

Price
 
 

TruSight Oncology 500 High-Throughput DNA/RNA for Automation (72 Samples)

20049284

Price
 
 

TSO500 DNA/RNA HT Auto (72 spls) + Velsera

20049278

Price
 
 

Index Adapters

IDT® for Illumina® UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)

20034701

Price
 
 

IDT® for Illumina® UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)

20034702

Price
 
 

IDT for Illumina UMI DNA/DNA Index Anchors Set A for Automation

20066404

Price
 
 

IDT for Illumina UMI DNA/DNA Index Anchors Set B for Automation

20063213

Price
 
 

Reagents

NovaSeq 6000 S4 Reagent Kit v1.5 (200 cycles)

20028313

Price
 
 

NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles)

20028315

Price
 
 

NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles)

20028318

Price
 
 

NovaSeq 6000 SP Reagent Kit v1.5 (200 cycles)

20040719

Price
 
 

Services

Trusight Oncology 500 HT Training (DNA) - Customer Site

20042220

Price
 
 

Trusight Oncology 500 HT Training (DNA and RNA) - Customer Site

20042219

Price
 
 

Software and Informatics Options

Illumina DRAGEN Server v3

20040619

Illumina DRAGEN Server v4

20051343

Price
 
 

ICA Basic Annual Subscription

20044874

ICA Professional Annual Subscription

20044876

ICA Enterprise Annual Subscription

20038994

ICA Enterprise Service and Compliance Add-on (applies to Basic only)

20066830

Illumina Analytics - 1 iCredit

20042038

Price
 
 

Illumina Analytics  Starter Pack - 1,000 iCredits

20042039

Price
 
 

Illumina Analytics  - 5,000 iCredits

20042040

Price
 
 

Illumina Analytics  - 50,000 iCredits

20042041

Price
 
 

Illumina Analytics - 100,000 iCredits

20042042

Price
 
 

Illumina Connected Insights-Annual Subscription

20090137

Illumina Connected Insights‒Oncology Genome Equivalent Sample-VCF

20090138

Illumina Connected Insights‒Training and Onboarding

20092376

Informatics Professional Services

20071787

Product Highlights

The TruSight Oncology 500 High-Throughput assay is built on the foundation of the TruSight Oncology 500 assay, with flexibility for higher sample throughput and batching options.

Consolidate Multiple Biomarker Assays into One

  • Pan-cancer biomarker content aligned with key guidelines and clinical trials
  • 523 genes for assessment of all DNA and RNA variant types, plus microsatellite instability (MSI) and tumor mutational burden (TMB)
  • Increase chances of finding a positive biomarker by moving from individual biomarker assays to a single comprehensive NGS assay

Streamlined Sample-to-Results Workflow

  • Go from initial sample to results in 4-5 days
  • Rapid variant calling algorithm powered by DRAGEN pipelines, available locally on DRAGEN server
  • Secure, scalable, cloud-based variant calling is available with DRAGEN pipelines on Illumina Connected Analytics (ICA) featuring auto-launch capability.
  • Multiple verified tertiary analysis options available, including Illumina Connected Insights and Pierian Clinical Genomics Workspace

High Sample Throughput

  • Flexible sample batching allows you to run anywhere from 16 to 192 samples per sequencing flow cell on the NovaSeq 6000 or NovaSeq 6000Dx System in RUO Mode

Achieve Highly Confident Results

  • Hybrid-capture chemistry combined with sophisticated bioinformatics, leading to high analytical specificity and sensitivity
  • Inclusive of unique molecular indexes (UMIs) for high sensitivity in variant detection
  • Based on proven Illumina SBS sequencing technology

Enable In-House Comprehensive Genomic Profiling

  • Keep samples and data in house, for more control and ability to build a database
  • A future-proof assay with emerging biomarkers already included

Automation

Automation is available on the Beckman Coulter i-Series or the Hamilton Microlab STAR.

Learn more about the TruSight Oncology Product Family

NovaSeq 6000Dx System in RUO Mode requires a separate, stand-alone DRAGEN server for secondary analysis.

Not available in all countries. Illumina Connected Insights supports user-defined tertiary analysis through API calls to third-party knowledge sources.

TruSight Oncology 500 Portfolio

All three TruSight Oncology 500 assays are designed to identify key biomarkers in guidelines and clinical trials.

TruSight Oncology 500 Assay

Assess key cancer biomarkers from FFPE tumor tissue with the TruSight Oncology 500 Assay.

TruSight Oncology 500 High-Throughput Assay

Batch up to 192 samples at a time while using the same biomarkers and tissue type as the TruSight Oncology 500 assay.

TruSight Oncology 500 ctDNA Assay

Analyze circulating tumor DNA from plasma with the TruSight Oncology 500 ctDNA assay.

Interested in learning more about the TSO 500 portfolio of products? Download the overview brochure.

Frequently Purchased Together

Specifications

Product Comparison

TruSight Oncology 500 High-Throughput TruSight Oncology 500 TruSight Oncology 500 ctDNA v2
Cancer Type Pan-Cancer Pan-Cancer Pan-Cancer, Solid Tumor
Content Specifications Targeted selection of DNA from 523 genes of interest, and RNA from 55 genes, for a total of 1.94Mb panel size.
• Immuno-oncology Biomarker Coverage: TMB and MSI
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Clinical Trials Coverage: Over 600 clinical trials*
Targeted selection of 523 genes (full coding sequence) for a total of 1.94Mb panel size.
• Immuno-oncology Biomarker Coverage: TMB and MSI
• Guideline Coverage: Broad coverage of key guidelines for multiple solid tumor types
• Clinical Trials Coverage: Over 600 clinical trials*
Hands-On Time Manual: ~10.5 hrs
Automated: ~2.5 hrs
Manual: ~10.5 hrs
Automated: ~2.5 hrs (TruSight Oncology 500 only), Manual: ~10.5 hrs
Automated: ~2.5 hrs (TruSight Oncology 500 only)
~2.5 hours for library prep and enrichment
Input Quantity 40 ng DNA, 40-80 ng RNA (need at least 2 mm3 FFPE tissue) 40 ng DNA, 40 ng RNA
Method Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing
Nucleic Acid Type RNA, DNA RNA, DNA DNA
Sample Throughput 16–192 samples per flow cell
Specialized Sample Types FFPE Tissue FFPE Tissue Blood, Circulating Tumor DNA
Species Category Human Human Human
System Compatibility NovaSeq 6000, NovaSeq 6000Dx in Research Mode NextSeq 500, NextSeq 550, NextSeq 550Dx in Research Mode NovaSeq 6000
Technology Sequencing Sequencing Sequencing
Variant Class Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants Copy Number Variants (CNVs), Gene Fusions, Insertions-Deletions (indels), Single Nucleotide Variants (SNVs), Transcript Variants Copy Number Variants (CNVs), Insertions-Deletions (indels), Single Nucleotide Variants (SNVs)
Variant Class Details For TruSight Oncology 500 HRD, genomic instability score, and the following beta features - tumor fraction, tumor ploidy, absolute copy number, and gene-level loss of heterozygosity - are available. Beta features have not been verified by Illumina. See the v2.5+ customer release notes for more details. For TruSight Oncology 500 HRD, genomic instability score, and the following beta features - tumor fraction, tumor ploidy, absolute copy number, and gene-level loss of heterozygosity - are available. Beta features have not been verified by Illumina. See the v2.5+ customer release notes for more details.

Based on Velsera clinical knowledgebase, as of February 2023.

NovaSeq 6000Dx System in RUO Mode requires a separate, stand-alone DRAGEN server for secondary analysis.

Method-Specific Workflow Example

Supporting Data and Figures

 

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