TruSight Tumor 15

Focused next-generation sequencing (NGS) panel to assess 15 commonly mutated genes in solid tumors with one simple and rapid workflow.

7 hr

Assay time

3.5 hr

Hands-on time

20 ng FFPE DNA 

Input quantity

See full details in the specifications table

Overview

TruSight Tumor 15 is an NGS assay that assesses 15 commonly mutated genes in solid tumors. 

  • Accurate, economical, and rapid assessment of 15 genes in a single assay 1 

  • Detection of somatic variants at 5% allele frequency using 20 ng DNA from FFPE tissue1

  • Easy-to-interpret report requiring minimal bioinformatics resources 

  • Seamless integration into existing lab practices 

Relevant gene content

Content is carefully selected to include genes and gene regions cited by industry organizations and informed by independent consortia publications and late-stage pharmaceutical research. Content includes single nucleotide variants (SNVs) and indels with demonstrated involvement in solid tumors.

Comprehensive workflow

The TruSight Tumor 15 assesses 15 genes in a simple, rapid workflow that replaces single, iterative gene testing with polymerase chain reaction (PCR). Analyze data with on-instrument reporting software or the TruSight Tumor 15 BaseSpace Sequence Hub App.  

Accelerated tumor profiling

Go from DNA to data in ~36 hours with only 3.5 hours of hands-on time. Maximize sample success rates across multiple tumor types to conserve limited samples and resources. 


Specifications


Required products

Requires purchase of either a TruSight Tumor 15 MiSeq or MiniSeq Kit, or purchase of TruSight Tumor 15 library prep only. All products are sufficient for 24 samples. If opting to purchase TruSight Tumor 15 library prep only, you will also need to separately purchase sequencing reagents for your system. 

The TruSight Tumor 15 MiSeq Kit requires MiSeq Software v2.6 or higher and the Illumina Annotation Engine. Download files

/ Results

Applications

TruSight Tumor 15 is a comprehensive workflow solution that assesses somatic variants in 15 genes commonly associated with solid tumors in a single assay, enabling labs to use the power of NGS to focus on genes most likely to play a role in tumorigenesis and confidently analyze low-frequency variants from FFPE DNA. 

Example workflow

1
Prep

TruSight Tumor 15


Project recommendations

Instrument Recommended number of samples Read length
MiniSeq System

Samples per run: mid output: 2-3, high output: 8 (based on 93.5% of the bases covered at 500x minimum)

2 × 150 bp (max recommended)

MiSeq System

8 samples per run with v3 reagents (based on 93.5% of the bases covered at 500x minimum)

2 × 151 bp (max recommended)


Related applications and methods

Compare

TruSight Tumor 15 TruSight Tumor 170
Assay time 7 hr ~2 days
Automation details Explore available automation methods Explore available automation methods
Cancer type Pan-Cancer, Solid Tumor Solid Tumor
Content specifications Amplifies 250 amplicons from 15 genes associated with solid tumors Probes enrich for full coding sequences of 170 genes. Calls single nucleotide variants, small insertions, and deletions in 151 genes, amplifications in 59 genes, and fusions plus splice variants in 55 genes.
Description

NGS assay for 15 commonly mutated genes in solid tumors using 20 ng DNA. 

Perform comprehensive somatic variant detection research in solid tumors using variant calling information from both DNA and RNA.
Hands-on time 3.5 hr ~10.5 hr
Input quantity

20 ng FFPE DNA 

40 ng DNA and/or RNA
Instruments MiSeq System, MiSeqDx in Research Mode, MiniSeq System NextSeq 550 System, NextSeq 500 System, HiSeq 2500 System
Method Targeted DNA sequencing, Amplicon sequencing Targeted DNA sequencing, Targeted RNA sequencing, Target enrichment
Multiplexing 1-24-plex Up to 32-plex for DNA using both index sets, up to 16-plex for RNA
Nucleic acid type DNA DNA, RNA
Specialized sample types FFPE Tissue Low-Input Samples, FFPE Tissue
Species category Human Human
Technology Sequencing Sequencing
Variant class Somatic variants, Insertions-deletions (indels) Gene fusions, Somatic variants, Structural variants, Single nucleotide variants (SNVs), Insertions-deletions (indels), Copy number variants (CNVs)

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Figures

TruSight Tumor 15 chemistry

The TruSight Tumor 15 assay uses a multiplex PCR approach, resulting in high accuracy and sensitivity.

TruSight Tumor 15 Gene List
AKT1 GNA11 NRAS
BRAF GNAQ PDGFRA
EGFR KIT PIK3CA
ERBB2 KRAS RET
FOXL2 MET TP53

TruSight Tumor 15 MiSeq Kit

OP-101-1001

Includes 1 library prep kit and 3 MiSeq reagents sufficient for 24 samples.

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TruSight Tumor 15 (Library Prep Only)

OP-101-1002

Includes 1 library prep kit sufficient for 24 samples.

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TruSight Tumor 15 MiniSeq Kit

20005610

Includes 1 library prep kit and 3 MiniSeq reagents sufficient for 24 samples.

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FAQs

TruSight Tumor 15 is supported on the MiniSeq System and MiSeq System (v3 chemistry), as well as on the MiSeqDx Instrument (in Research Mode; v3 chemistry). Sequencing on other Illumina systems is possible but has not been tested.

The following thermal cyclers are recommended for use with the TruSight Tumor 15 kit. Follow the recommended ramp rate setting listed in the TruSight Tumor 15 Reference Guide.

  • Bio-Rad C1000

  • Bio-Rad S1000 

  • Applied Biosystems GeneAmp PCR System 9700

  • Applied Biosystems Veriti Thermal Cycler 

  • Eppendorf Mastercycler ep Gradient

  • Eppendorf Mastercycler ep Gradient-S 

  • Bio-Rad C1000, Applied Biosystems GeneAmp PCR System 9700, and Eppendorf MasterCycler ep Gradient were qualified internally

TruSight Tumor 15 targets 15 commonly mutated genes in solid tumors using amplicon sequencing and targeted DNA sequencing on the MiniSeq, MiSeq, and MiSeq Dx (v3 chemistry) Systems. TruSight Tumor 170 targets 170 genes using target enrichment and targeted DNA and RNA sequencing on the NextSeq 500 and NextSeq 550 Systems. 

Yes, you may view a sample run and a sample project.  A BaseSpace Sequence Hub login is required to access this data.

/ Results

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Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

References

1. Data calculations on file. Illumina, Inc. 2016.

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