Ultra-scalable methylation array optimized for population health research. Ideal for studies >1,000 samples, up to millions of samples.
The Infinium Methylation Screening Array is an affordable and highly scalable epigenomics tool for population health research.
| Automation capability | Liquid Handling Robots, Automated Array Loader |
|---|---|
| Description |
Highly scalable and affordable methylation analysis of known common disease associations for population health applications. Ideal for studies >1,000 samples and up to millions of samples. |
| Input quantity | 50 ng DNA |
| Instruments | iScan System |
| Method | Methylation Array |
| Number of samples | 48 samples per array |
| Sample throughput |
16,128 samples per week |
| Specialized sample types | Low-Input Samples |
| Species category | Human |
| Technology | Microarray |
| Variant class | Differentially methylated cytosines |
The Infinium Automated Pipetting System with ILASS and iScan are required for the EX Methylation workflow. The Infinium EX/ XT starter kit is required for customers who are new to Infinium and do not already have a XT starter kit. The AutoLoader 2.x is an optional device for automatic loading of beadchips onto the iScan.
The Methylation Screening Array enables affordable, large-scale epigenetic analysis for population health studies.
Ex Methylation Assay
Genome-wide methylation profiling studies with microarray and NGS technologies can provide novel insights into the functional impact of methylation.
Illumina offers solutions for epigenetic analysis. Use our tools to study epigenetic modifications and their impact on gene regulation.
Methylation arrays enable high-throughput, quantitative interrogation of methylation sites across the genome at single-nucleotide resolution.
| Infinium Methylation Screening Array-48 Kit | Infinium MethylationEPIC v2.0 Kit | |
|---|---|---|
| Automation capability | Liquid Handling Robots, Automated Array Loader | |
| Description |
Highly scalable and affordable methylation analysis of known common disease associations for population health applications. Ideal for studies >1,000 samples and up to millions of samples. |
The Infinium MethylationEPIC v2.0 BeadChip is a genome-wide methylation screening tool with enhanced, expert-selected functional content. The kit is processed on the iScan or NextSeq 550 Systems and follows a straightforward, user-friendly workflow that does not require sample pooling and indexing. |
| Input quantity | 50 ng DNA | 250 ng DNA |
| Instruments | iScan System | |
| Method | Methylation Array | |
| Number of samples | 48 samples per array | 8 samples per array |
| Sample throughput |
16,128 samples per week |
> 1000 samples per week (per Tecan for 8 sample BeadChip) |
| Specialized sample types | Low-Input Samples | |
| Species category | Human | |
| Technology | Microarray | |
| Variant class | Differentially methylated cytosines |
Library prep and array kit selector
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Highly reproducible methylation results—(A) Methylation results are highly correlated between Infinium Methylation Screening Array and Infinium MethylationEPICv2.0 BeadChips. (B) Methylation results demonstrate excellent reproducibility between replicate samples analyzed on the Infinium Methylation Screening Array.
Markers on the Infinium Methylation Screening Array are highly enriched in known trait-associated CpGs across diverse trait types—Markers on the Methylation Screening Array (MSA) are selected based on evidence for trait association, shown in comparison with trait enrichment on Infinium MethylationEPICv2.0 (EPICV2).
| Infinium Methylation Screening Array-48 Targeted methylation screening for population health research |
|
|---|---|
| Recommended applications | Common disease research (noncancer) Environmental epidemiology Population genomics Consumer genomics |
| Content focus | Known common disease trait associations Known environmental exposure associations Cell-type specific methylation Intermediate methylation Multiomic capabilities to measure high MAF SNPs |
| Total methylation sites | 270K |
| Number of samples per BeadChip | 48 |
| DNA input requirement | 50 ng |
| Assay chemistry | Infinium EX Methylation |
| Instrument support | iScan |
| iScan System maximum sample throughputa | 16,128 samples/week |
| Liquid Handling Automation | Infinium Automated Pipetting System with ILASS (required) |
a Approximate values, scan times, and maximum throughput will vary depending on laboratory and system configurations. Sample throughput listed here is achieved with integration of AutoLoader 2.x automated array loading.
| Infinium Methylation Screening Array | Infinium MethylationEPIC v2.0 | |
|---|---|---|
| Total unique sites | 269,094 | 930,301 |
| CpG | 262,470 | 926,849 |
| From Infinium methylation arrays | 161,569 | - |
| From sequencing studies and databases | 101,901 | - |
| CpH | 2776 | 2914 |
| From Infinium methylation arrays | 308 | - |
| From sequencing studies and databases | 2468 | - |
| SNP rsID | 3848 | 538 |
| From Infinium methylation arrays | 64 | |
| From sequencing studies and databases | 3784 |
| Trait category | No. of probes targeting trait association |
|---|---|
| Development/aging | 102,533 |
| Environmental exposures | 44,043 |
| Inflammation/autoimmune diseases | 41,894 |
| Ancestry | 31,843 |
| Sex | 23,806 |
| Infectious diseases | 14,844 |
| Metabolic diseases | 13,739 |
| Rare genetic disorders | 13,429 |
| Neurological/neurodevelopmental diseases | 8874 |
| Body features (body morphology) | 8109 |
| Psychiatric disorders | 7280 |
| Cardiovascular disease | 7007 |
| Reproductive biology/health | 6999 |
| Neurodegenerative diseases | 4733 |
| Lung/respiratory diseases | 1748 |
| Renal disease | 982 |
Infinium Methylation Screening Array-48 v1.0 Kit (48 samples)
20112611
Optimized methylation array with 252K markers for high-throughput common disease research <(>&<)> screening. Kit contains 1 48-sample BeadChip and all required reagents for processing 48 samples after bisulfite conversion. Requires iScan System and Infinium Automated Pipetting System with ILASS.
Infinium Methylation Screening Array-48 v1.0 Kit (96 samples)
20112612
Optimized methylation array with 252K markers for high-throughput common disease research <(>&<)> screening. Kit contains 2 48-sample BeadChips and all required reagents for processing 96 samples after bisulfite conversion. Requires iScan System and Infinium Automated Pipetting System with ILASS.
Infinium Methylation Screening Array-48 v1.0 Kit (1,152 samples)
20112613
Optimized methylation array with 252K markers for high-throughput common disease research <(>&<)> screening. Kit contains 24 48-sample BeadChips and all required reagents for processing 1,152 samples after bisulfite conversion. Requires iScan System and Infinium Automated Pipetting System with ILASS.
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The Infinium MethylationEPIC v2.0 BeadChip offers a comprehensive genome-wide discovery tool with ~930K CpG sites covering >99% RefSeq genes with promoter and enhancer-focused content. The Infinium MethylationEPIC v2.0 BeadChip is ideal for projects in which methylation profiling across all genes and promoter regions is desired for maximum discovery power. It is also ideal for all oncology applications due to its coverage of critical oncogenes, compatibility with FFPE tissue, and copy number variation profiling capabilities.
The Infinium Methylation Screening Array offers a more cost-effective methylation screening tool for the study of common disease for population health projects. The ~270K methylation sites are focused on known trait associations from epigenome-wide association studies over the past decade, as well as novel content covering cell-type specific methylation, intermediate methylation, CoRSIVs, SNPs, and more. The Infinium Methylation Screening Array is ideal for large-scale epigenome-wide association studies (EWAS) and biobank screening projects.
A comprehensive product comparison table can be found on the Infinium Methylation Screening Array datasheet
Yes, the Methylation Screening Array requires the EX Methylation workflow while the EPICv2.0 uses HD Methylation workflow. EX Methylation delivers new sample processing efficiencies enabled by the 48 sample EX BeadChip and automation., and has a lower DNA input of 50ng. The EX Methylation workflow requires the Infinium Automated Pipetting System with ILASS for automated liquid handling.
While the Illumina product manifest file provides basic genomic mapping annotations, the Zhou Lab Infinium Annotations GitHub page provides detailed annotations including trait associations covered on the Infinium Methylation Screening Array.
The Infinium Methylation Screening Array is not recommended for oncology applications due to the following reasons:
· FFPE compatibility unvalidated
· Does not have sufficient coverage of probes for widely-used cancer classifiers
· Does not cover MGMT promoter methylation
· Limited ability to profile copy number variations
· Larger minimum batch size of 48 samples per BeadChip may result in longer turnaround times for results
Illumina offers user friendly tools to analyze Infinium controls built into the Methylation Screening Array. For more information visit the Methylation Array Data Analysis Tips page.
Yes, service labs can use the GenomeStudio Methylation Module Controls Dashboard for assessing assay performance. For downstream methylation analysis, Bioconductor packages such as SeSAMe are recommended.
Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Enable CGP with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD) from FFPE tissue.
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Robust methylation profiling microarray providing extensive coverage of CpG islands, genes, and enhancers for epigenome-wide association studies.
Features > 285k markers across the methylome for high-resolution epigenetic analyses of diverse murine strains.
Fully customizable methylation microarray for large, targeted epigenetic applications.
Illumina genotyping automation options for Infinium, GoldenGate, and DASL Assays streamline workflow and reduce errors. Illumina robot control software, together with liquid-handling automation, enables seamless automation of sample preparation into the iScan System workflow.
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