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HiSeq X Reagent Kits

Kits include SBS reagents, clustering reagents, and patterned flow cells for increased cluster density and simplified image analysis, compared to non-patterned flow cells.

The HiSeq X Five and Ten Systems have been discontinued. We will continue to provide full support of the instruments through March 31, 2024. To find an alternative instrument, we recommend using the Sequencing Platform Comparison Tool.

Overview

HiSeq X Reagent Kits support high coverage whole-genome sequencing (WGS) with the HiSeq X Series of sequencing systems. By leveraging patterned flow cells, these kits provide increased cluster density to enable significant increases in data output and daily throughput. HiSeq X Reagent Kits support industry-leading library preparation options while offering superior data quality and coverage.

HiSeq X Reagent Kits support the TruSeq DNA PCR-Free Library Preparation Kit, for industry-best coverage of challenging genomic regions, and the TruSeq Nano DNA Library Preparation Kit, for efficient sequencing of samples with limited available DNA. Together, the reagent kits and library preparation options deliver exceptional genome coverage, enabling highly accurate and comprehensive variant calling for WGS on the HiSeq X Ten System. The HiSeq X Reagent Kits:

  • Deliver ultra-high data output

  • Provide ultra-high daily throughput

  • Enable sequencing up to 16 genomes per run

  • Are available in single and multipack configurations

  • Are compatible with TruSeq DNA PCR-Free and TruSeq Nano DNA library preparation kits

HiSeq X Reagent Kits are only compatible with the HiSeq X Series of sequencing systems. HiSeq X Ten Reagent Kits are only available for purchase by HiSeq X Ten users and the HiSeq X Five Reagent Kits are only available for purchase by HiSeq X Five users. Reagents are also available in a multipack configuration to support bulk purchasing with significant reductions in packaging materials.

Latest technology innovation powers population-scale sequencing

HiSeq X Reagent Kits leverage the newest format in Illumina flow cell technology to achieve ultra-high-throughput WGS. Patterned flow cells contain billions of nanowell substrates at fixed locations across both surfaces of the flow cell. The structured organization (see figures) provides even spacing of sequencing clusters to deliver significant increases in sequencing reads and total output of the system.

Specifications

Maximum output Up to 1800 Gb (1 HiSeq X System with dual flow cells)
Maximum reads per run Up to 6 billion
Nucleic acid type DNA, RNA
Reagent type Paired-end sequencing, Cluster generation
Technology Sequencing

Applications

Related applications and methods

Sequencing platforms

Compare next-generation sequencing (NGS) platforms by application, throughput, and other key specs.

Patterned flow cell technology

Fixed spacing of sequencing clusters with defined feature sizes contributes to increased data output, reduced costs, and faster run times.

Sequencing

Illumina sequencing allows researchers to ask virtually any question related to the genome, transcriptome, or epigenome of any organism.

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

Figures

Advanced patterned flow cell design enables maximum throughput

Patterned flow cells contain billions of nanowells at fixed locations providing even cluster spacing and uniform feature size to deliver extremely high cluster density.

Resources

Best practices for storing sequencing reagents

Sequencing reagent performance depends on thawing technique, thawed storage time, and storage conditions. Learn how to thaw and store sequencing reagents for optimal performance.

HiSeq X Ten Reagent Kit v2.5

FC-501-2501

Includes two flow cells with clustering reagents for use on the cBot Systems and two sequencing reagents to support 2 × 300-cycle runs on the HiSeq X Ten System.

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HiSeq X Ten Reagent Kit v2.5 - 10 pack

FC-501-2521

Includes 20 flow cells with clustering reagents for use on the cBot Systems and 20 sequencing reagents to support 20 × 300-cycle runs on the HiSeq X Ten System.

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HiSeq X Five Reagent Kit v2.5

FC-502-2501

Includes two flow cells with clustering reagents for use on the cBot Systems and two sequencing reagents to support 2 × 300-cycle runs on the HiSeq X Five System.

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HiSeq X Five Reagent Kit v2.5 - 10 pack

FC-502-2521

Includes 20 flow cells with clustering reagents for use on the cBot Systems and 20 sequencing reagents to support 20 × 300-cycle runs on the HiSeq X Five System.

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Trade in your HiSeq instrument for more discovery power.

If you currently own a HiSeq 2500, HiSeq 3000/4000, or HiSeq X, you may be eligible to trade in your existing instrument for a new NovaSeq 6000 System. This program is available for a limited time only. Terms and conditions apply. Fill out the form below to contact a sales representative to see if you are eligible.

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Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

Illumina DNA Prep

A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.

TruSeq DNA Nano

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.

TruSeq DNA PCR-Free

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.

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