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PhiX Control v3

PhiX Control v3 is a ready-to-use control library for Illumina sequencing runs.

Overview

PhiX Control v3 is a reliable, adapter-ligated library used as a control for Illumina sequencing runs. The library is derived from the small, well-characterized PhiX genome, offering several benefits for sequencing and alignment.

The versatile PhiX Control v3 is provided as a ready-to-use library, and can be utilized in diverse applications to add value to your workflow and increase confidence in your results.

The PhiX library provides a quality control for cluster generation, sequencing, and alignment, and a calibration control for cross-talk matrix generation, phasing, and prephasing. It can be rapidly aligned to estimate relevant sequencing by synthesis (SBS) metrics such as phasing and error rate.

Depending on the application, PhiX Control v3 may also be utilized:

  • As a high-concentration spike-in control for unbalanced samples (genomes with AT or GC content of less than 40% or greater than 60%)

  • As a low-concentration spike-in control for alignment calculations and quantification efficiency

  • As a dedicated control lane alongside low-diversity samples

  • As a control for troubleshooting cluster generation problems, to help determine whether an error is related to library preparation

Specifications

Instruments MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiSeqDx in Research Mode, MiniSeq System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System, MiSeq i100 System, MiSeq i100 Plus System
Nucleic acid type DNA
Reagent type Sequencing control
Technology Sequencing

Documentation

Product literature

Literature is not currently available for this product.

Support documentation

Resources

What is PhiX Control v3 and what is its function in Illumina sequencing?

Learn more about the PhiX Control v3 library and how it's used as a run quality monitor, calibration control, and more. 

PhiX loading concentrations for Illumina sequencing validation runs

See what the optimal loading concentrations and cluster densities are for each Illumina sequencing platform.

PhiX Control v3

FC-110-3001

Kitted DNA control for the Illumina sequencing platform. Compatible with Single and Paired End reads up to 150 base pairs. (10ul of 10nM template solution)

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TG PhiX Control Kit v3

TG-110-3001

PhiX Control Kit v3

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NextSeq PhiX Control Kit

FC-110-3002

DNA control for the NextSeq Illumina sequencing platform. Compatible with single and paired-end reads up to 150 bp. 10 µl of a 10 nM PhiX solution.

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